Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Immuno-enhancing Effect of Seed Extracts on a RAW 264.7 Macrophage Cell Line

종자 추출물의 RAW 264.7 세포에 대한 면역증강 효과

  • Received : 2012.10.24
  • Accepted : 2012.11.21
  • Published : 2012.12.31
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Abstract

In this study, the immuno-enhancing activity of seed extracts were studied on the macrophage cell lines. We examined the effect of nine seed extracts on nitric oxide (NO) production in RAW 264.7 cells and selected four highly-effective seed candidates (Fagopyrum esculentum, Taraxacum platycarpum, Impatiens balsamina, Helianthus annuus) for further immune-related studies. The effects of the four seed extracts on the production of immune-related cytokines in the RAW 264.7 macrophage cell line and proliferation of Molt-4 as a T cell line were investigated. The secretion of NO from the RAW 264.7 cells was increased up to 39 ${\mu}M$ by adding the seed extracts (25 ${\mu}g/mL$) compared to the control. Also, the secretion of tumor necrosis factor-alpha (TNF-${\alpha}$) was also increased up to 32 times by adding the seed extracts (25 ${\mu}g/mL$). Secretion of cytokines such as interleukin-1 beta (IL-$1{\beta}$), interleukin-6 (IL-6), and interleukin-10 (IL-10) was also increased and induced the proliferation of T cells compared to the control. In conclusion, these results suggest that four seed extracts provide beneficial immuno-enhancing effects for human health.

9 종류의 종자 중 대식세포를 자극하여 NO 생성능이 높은 메밀, 민들레, 봉선화, 해바라기 4종의 종자를 선별하였다. 선별된 4종의 종자 추출물이 RAW 264.7 세포를 활성화시켜 면역을 증진시키는 효과를 알아보기 위해 RAW 264.7 세포와 T세포를 이용하여 면역 활성능 관련 지표를 조사하였다. 4종의 종자를 대식세포에 처리하였을 때 면역 활성의 지표가 되는 NO, cytokine(TNF-${\alpha}$, IL-$1{\beta}$, IL-6, IL-10)의 생성이 추출물을 처리하지 않은 대조군에 비해 증가되었고, Molt-4 세포에 처리하였을 때 대조군에 비해 세포가 증식되었다. 이와 같은 결과는 종자 추출물을 섭취하였을 때 외부로부터의 어떠한 자극이 있기 이전에 체내의 모든 조직에 분포하면서 1차적으로 이물질을 제거하는 대식세포를 자극하여 cytokine 등의 면역매개물질을 생성하여 인체의 비특이적 면역반응을 증가시킴으로써 항원을 공격, 제거하는 등의 작용을 통해 자연 면역반응에 있어 중요한 역할을 할 수 있을 것으로 판단된다.

Keywords

References

  1. Seo SY, Pang JY, Li RH, Kwon J, Ahn MS, Eun JS. 2009. Effects of the combined extracts of Glycine max Merr. and Glycyrrhiza uralensis on the activity of murine splenocytes and macrophages. Korean J Oriental Physiol Pathol 23: 1385-1391.
  2. Hibbs JB Jr, Taintor RR, Vavrin I, Rachlin EM. 1998. Nitric oxide: a cytotoxic activated macrophage effector molecule. Biochem Biophys Res Commun 157: 87-94.
  3. Nathan CF. 1987. Secretory products of macrophages. J Clin Invest 79: 319-326. https://doi.org/10.1172/JCI112815
  4. Yoon TJ. 2008. Effect of water extracts from root of Taraxacum officinale on innate and adaptive immune responses in mice. Korean J Food Nutr 21: 275-282.
  5. Jeon HS, Lee YS, Kim NW. 2009. The antioxidative activities of Torreya nucifera seed extracts. J Korean Soc Food Sci Nutr 38: 1-8. https://doi.org/10.3746/jkfn.2009.38.1.001
  6. Kim MJ, Hong CO, Nam MH, Lee KW. 2011. Antioxidant effects and physiological activities of pumpkin (Cucurbita moschata Duch.) extract from different aerial parts. Korean J Food Sci Technol 43: 195-199. https://doi.org/10.9721/KJFST.2011.43.2.195
  7. Ku KH, Choi EJ, Park WS. 2008. Functional activity of water and ethanol extracts from red pepper (Capsicum annuum L.) seeds. J Korean Soc Food Sci Nutr 37: 1357-1362. https://doi.org/10.3746/jkfn.2008.37.10.1357
  8. Yang HS, Kim JY, Kim HC, Nou IS, Seo KI. 2006. Biological activities of methanol extracts from green tea seed. Korean J Food Preserv 13: 769-773.
  9. Kim HJ, Jun BS, Kim SK, Cha JY, Cho YS. 2000. Polyphenolic compound content and antioxidative activities by extracts from seed, sprout and flower of safflower (Carthamus tinctorius L.). J Korea Soc Food Sci Nutr 29: 1127-1132.
  10. Kim HS, Kang YH. 2010. Antioxidant activity of ethanol extracts of non-edible parts (stalk, stem․leaf, seed) from oriental melon. Korean J Plant Res 23: 451-457.
  11. Bae CH, Kim JC, Kim YJ, Kim SG, Na KH, Park BT, Kim HH. 2007. Purification efficiency of a lectin from Maackia fauriei. Yakhak Hoeji 51: 259-263.
  12. Bae IK, Min HY, Han AR, Seo EK, Lee SK. 2005. Suppression of lipopolysaccharide-induced expression of inducible nitric oxide synthase by brazilin in RAW 264.7 macrophage cells. Eur J Pharmacol 513: 237-242. https://doi.org/10.1016/j.ejphar.2005.03.011
  13. Kim YH, Yoon HJ, Moon ME, Lee JH, Park HS, Kim JS. 2005. Production of NO, TNF-${\alpha}$ and IL-6 by squalene, alkoxy glycerol, batyl and chimyl solutions in Raw 264.7 macrophage cells. J Korean Soc Food Sci Nutr 34: 1503-1508. https://doi.org/10.3746/jkfn.2005.34.10.1503
  14. Ryu HS, Kim JH, Kim HS. 2008. Effects of plant water extract mixture Ixeris sonchifolia Hance, Oenanthev javanica, Fagopyrum esculentum Moench, Hizikia fusiforme, Zingiber officinale Roscoe on mouse immune cell activation ex vivo. Korean J Nutr 41: 141-146.
  15. Shin KS. 2012. Immunostimulating plant polysaccharides: macrophage immunomodulation and its possible mechanism. Food Sci Ind 45: 12-22.
  16. Ryu HS, Kim HS. 2006. Effect of Zingiber officinale and Hizikia fusiforme water extracts on NO production in macrophage of mice. Korean J Food & Nutr 19: 327-331.
  17. Lee JB, Yamagishi C, Hayashi K, Hayashi T. 2011. Antiviral and immunostimulating effects of lignin-carbohydrateprotein complexes from Pimpinella anisum. Biosci Biotechnol Biochem 75: 459-465. https://doi.org/10.1271/bbb.100645
  18. Tong H, Song X, Sun X, Sun G, Du F. 2011. Immunomodulatory and antitumor activities of grape seed proanthocyanidins. J Agric Food Chem 59: 11543-11547. https://doi.org/10.1021/jf203170k
  19. Kang NS, Sohn EH. 2010. Immunomodulatory effects of fructus and semen from Rosa rugosa on macrophages. Korean J Plant Res 23: 399-405.
  20. Bahaa KA, Abel S. 2011. Immunomodulatory effects of black seeds and garlic on alloxan-induced diabetes in albino rat. Allergol Immunopathol 324: 1-5.
  21. Jeong HJ, Chung HS, An HJ, Kim JB, Lee EM, Park EJ, Jang CH, Hong SH, Kim HM. 2003. Immune-enhancement effect of the herbal combination Allergina. Clin Chim Acta 337: 77-84. https://doi.org/10.1016/j.cccn.2003.07.001
  22. Cha JH, Kim YS, Lee EM. 2010. Effects of Prunellae spica water extract on immune response in macrophage cells. J Orien Obstet Gynecol 23: 91-100.
  23. Kim JH, Kim YS, Lim EM. 2012. Immune enhancing effect of Houttuyniae herba on mouse macrophage. J Orein Obstet Gyneconl 25: 12-22.
  24. Majdalawieh AF, Carr RI. 2010. In vitro investigation of the potential immunomodulatory and anti-cancer activities of black pepper (Piper nigrum) and cardamom (Elettaria cardamomum). J Med Food 13: 371-381. https://doi.org/10.1089/jmf.2009.1131
  25. Wang TH, Kung YL, Lee MH, Su NW. 2011. N-Acetyl-Dgalactosamine- specific lectin isolated from the seeds of Carica papaya. J Agric Food Chem 59: 4217-4224. https://doi.org/10.1021/jf104962g
  26. Seo YS, Shin KS. 2012. Immune system-stimulating activities of mucilage polysaccharides isolated from Opuntia humifusa. J Korean Soc Food Sci Nutr 41: 95-102. https://doi.org/10.3746/jkfn.2012.41.1.095
  27. Park SH, Choung SY, Choi YJ. 2011. Immune regulating effect of polysaccharide fraction from sea hare (Aplysia kurodai ). J Korean Soc Food Sci Nutr 40: 372-378. https://doi.org/10.3746/jkfn.2011.40.3.372

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