Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Gibberellin A4 Producted by Fusarium solani Isolated from the Roots of Suaeda japonica Makino

칠면초의 뿌리로부터 분리된 Fusarium solani에 의해 생산된 지베렐린 A4

  • Seo, Yeonggyo (School of Life Sciences, Kyungpook National University) ;
  • You, Young-Hyun (School of Life Sciences, Kyungpook National University) ;
  • Yoon, Hyeokjun (School of Life Sciences, Kyungpook National University) ;
  • Kang, Sang-Mo (School of Applied Biosciences, Kyungpook National University) ;
  • Kim, Hyun (School of Life Sciences, Kyungpook National University) ;
  • Kim, Miae (School of Life Sciences, Kyungpook National University) ;
  • Kim, Changmu (National Institute of Biological Resource) ;
  • Lee, In-Jung (School of Applied Biosciences, Kyungpook National University) ;
  • Kim, Jong-Guk (School of Life Sciences, Kyungpook National University)
  • 서영교 (경북대학교 생명과학부) ;
  • 유영현 (경북대학교 생명과학부) ;
  • 윤혁준 (경북대학교 생명과학부) ;
  • 강상모 (경북대학교 응용생명과학부) ;
  • 김현 (경북대학교 생명과학부) ;
  • 김미애 (경북대학교 생명과학부) ;
  • 김창무 (국립생물자원관 미생물자원과) ;
  • 이인중 (경북대학교 응용생명과학부) ;
  • 김종국 (경북대학교 생명과학부)
  • Received : 2012.10.24
  • Accepted : 2012.12.18
  • Published : 2012.12.30
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Abstract

Ten endophytic fungi with different colony morphologies were isolated from the roots of Suaeda japonica Makino growing naturally in Suncheon Bay. Plant growth promotion was verified by treating waito-c rice seedlings with culture filtrates from the endophytic fungi. The bioassays showed that the Sj/7/4 fungal strain induced effective growth promotion in the seedlings. The gibberellins (GA) produced by fungal strain Sj/7/4 were analyzed by gas chromatography /mass spectroscopy with selected ion monitoring (GC/MS SIM). The culture filtrate of Sj/7/4 fungal strain was confirmed to contain $GA_4$ through quantitative analysis. The Sj/7/4 fungal strain was identified to determine the internal transcribed spacer (ITS) regions with universal primers ITS-1 and ITS-4 by using polymerase chain reactions (PCR). Molecular analysis of the Sj/7/4 fungal strain showed high similarity to Fusarium solani. The Sj/7/4 fungal strain isolated from the root of S. japonica was therefore designated as F. solani Sj/7/4.

순천만에 자생하는 염생식물인 칠면초의 뿌리로부터 형태적으로 다른 내생진균을 분리하였다. 10종의 내생진균의 식물생장촉진활성검증을 위하여 내생진균의 배양여과액을 난장이벼 유묘에 처리하였다. Bioassay 결과, Sj/7/4 균주가 다른 내생진균류들보다 난장이벼의 생장을 효과적으로 촉진하는 것을 확인하였다. Sj/7/4 균주가 생산하는 GA를 확인하기 위하여 GC/MS SIM을 사용하여 분석하였다. 그 결과, $GA_4$를 생산하는 것이 정량분석을 통하여 확인되었다. 분리된 내생진균은 universal primers ITS-1과 ITS-4를 사용하여 ITS 영역을 PCR로 증폭하여 분석하였다. Sj/7/4 균주의 염기서열분석은 Fusarium solani와 높은 유사성을 나타냈다. 칠면초의 뿌리로부터 분리한 Sj/7/4 균주는 F. solani Sj/7/4로 명명하였다.

Keywords

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