생명과학회지 (Journal of Life Science)

  • 제22권12호
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  • Pages.1665-1671
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  • 2012
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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세척방법에 따른 양파껍질추출물의 Quercetin수율 및 잔류농약 제거효과

Reduction in Residual Pesticides and Quercetin Yields in Onion Peel Extracts by Washing

  • Jeong, Eun-Jeong (Department of Food and Nutrition, Changwon National University) ;
  • Cha, Yong-Jun (Department of Food and Nutrition, Changwon National University)
  • 투고 : 2012.10.24
  • 심사 : 2012.12.14
  • 발행 : 2012.12.30
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초록

본 연구는 양파껍질로부터 건강기능성식품 기능성원료로서의 양파껍질추출물의 표준화공정을 설정하고자 하였으며, 제조과정 중 지표물질의 추출효율 증대 및 잔류농약 안정성 확보를 위해 여러 세척공정을 통한 quercetin 함량 및 잔류농약 잔존율을 분석하였다. 양파껍질(A)을 1차 1종세척제(이하 세척제) 수세후 건조한 처리구(B), 2차 세척제 처리구(C), 초음파+세척제 처리구(D), 과산화수소+세척제 처리구(E) 및 데치기+세척제 처리구(F)로 처리한 다음 주정추출후 농축 및 진공동결건조하여 양파껍질추출분말을 제조하였다. 양파껍질추출물의 수율 및 지표물질인 quercetin의 수율을 고려하면 과산화수소처리+세척제 처리구(E)가 B처리구에 비해 각각 89.04% 및 96.84%로 추출효율이 가장 높게 나타났다. 모든 시료에서 총 177종의 농약성분 중 cyhalothirn, fluquinconazole 및 procymidone, 3종만이 검출되었는데, cyhalothirn (생양파 허용기준; 0.5 mg/kg), fluquinconazole (0.2 mg/kg)은 각각 0.37-0.68 mg/kg 및 0.35-3.28 mg/kg으로 농축도(27-60배)를 고려하면 허용기준에 안정하였으나 procymidone은 함량이 128.01~133.67 mg/kg으로 높았다. 따라서 양파껍질추출물의 표준화공정을 위해서는 추가적인 물리적 전처리 공정이 필요한 것으로 사료되었다.

This study was conducted to assess the removal of residual pesticides and to obtain high amounts of quercetin in onion peel extracts (OPEs) by 4 washing treatments. Washing is one of the standard processing steps in obtaining functional food ingredients from onion peel. After a first detergent wash (0.2% w/v) (DW) and hot air drying ($80^{\circ}C$, 24 hr) (B), 4 washing treatments were tested, including a second DW (C), ultrasonication ($50^{\circ}C$, 10 min) plus DW (D), 0.3% $H_2O_2$ (v/v) plus DW (E), and blanching ($95-97^{\circ}C$, 2 min) plus DW (F). This was followed by 60% (v/v) ethanol extraction and vacuum freeze drying of the OPE. The E treatment yielded 89.04% OPE and a quercetin content of 96.84% in the OPE compared with the B treatment, and had the highest efficiency of all treatments tested. The OPE was tested for the presence of 177 residual pesticides and three compounds were detected in all treatments: cyhalothirn, fluquinconazole and procymidone. Cyhalothirn and fluquinconazole levels were below the permitted levels for fresh onion, while procymidone was present in the high level range of 128.01~133.46 mg/kg in all samples. The E treatment was a better washing method than the others for removal of residual pesticides. It could reduce the level of residual pesticides without changing the functional properties of the OPE.

키워드

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피인용 문헌

  1. Study on the Synthesis of Isoquercitrin by Enzymatic Partial Hydrolysis vol.25, pp.2, 2014, https://doi.org/10.14478/ace.2013.1119