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세척방법에 따른 양파껍질추출물의 Quercetin수율 및 잔류농약 제거효과

Reduction in Residual Pesticides and Quercetin Yields in Onion Peel Extracts by Washing

  • Jeong, Eun-Jeong (Department of Food and Nutrition, Changwon National University) ;
  • Cha, Yong-Jun (Department of Food and Nutrition, Changwon National University)
  • 투고 : 2012.10.24
  • 심사 : 2012.12.14
  • 발행 : 2012.12.30

초록

본 연구는 양파껍질로부터 건강기능성식품 기능성원료로서의 양파껍질추출물의 표준화공정을 설정하고자 하였으며, 제조과정 중 지표물질의 추출효율 증대 및 잔류농약 안정성 확보를 위해 여러 세척공정을 통한 quercetin 함량 및 잔류농약 잔존율을 분석하였다. 양파껍질(A)을 1차 1종세척제(이하 세척제) 수세후 건조한 처리구(B), 2차 세척제 처리구(C), 초음파+세척제 처리구(D), 과산화수소+세척제 처리구(E) 및 데치기+세척제 처리구(F)로 처리한 다음 주정추출후 농축 및 진공동결건조하여 양파껍질추출분말을 제조하였다. 양파껍질추출물의 수율 및 지표물질인 quercetin의 수율을 고려하면 과산화수소처리+세척제 처리구(E)가 B처리구에 비해 각각 89.04% 및 96.84%로 추출효율이 가장 높게 나타났다. 모든 시료에서 총 177종의 농약성분 중 cyhalothirn, fluquinconazole 및 procymidone, 3종만이 검출되었는데, cyhalothirn (생양파 허용기준; 0.5 mg/kg), fluquinconazole (0.2 mg/kg)은 각각 0.37-0.68 mg/kg 및 0.35-3.28 mg/kg으로 농축도(27-60배)를 고려하면 허용기준에 안정하였으나 procymidone은 함량이 128.01~133.67 mg/kg으로 높았다. 따라서 양파껍질추출물의 표준화공정을 위해서는 추가적인 물리적 전처리 공정이 필요한 것으로 사료되었다.

This study was conducted to assess the removal of residual pesticides and to obtain high amounts of quercetin in onion peel extracts (OPEs) by 4 washing treatments. Washing is one of the standard processing steps in obtaining functional food ingredients from onion peel. After a first detergent wash (0.2% w/v) (DW) and hot air drying ($80^{\circ}C$, 24 hr) (B), 4 washing treatments were tested, including a second DW (C), ultrasonication ($50^{\circ}C$, 10 min) plus DW (D), 0.3% $H_2O_2$ (v/v) plus DW (E), and blanching ($95-97^{\circ}C$, 2 min) plus DW (F). This was followed by 60% (v/v) ethanol extraction and vacuum freeze drying of the OPE. The E treatment yielded 89.04% OPE and a quercetin content of 96.84% in the OPE compared with the B treatment, and had the highest efficiency of all treatments tested. The OPE was tested for the presence of 177 residual pesticides and three compounds were detected in all treatments: cyhalothirn, fluquinconazole and procymidone. Cyhalothirn and fluquinconazole levels were below the permitted levels for fresh onion, while procymidone was present in the high level range of 128.01~133.46 mg/kg in all samples. The E treatment was a better washing method than the others for removal of residual pesticides. It could reduce the level of residual pesticides without changing the functional properties of the OPE.

키워드

참고문헌

  1. Augusti, K. T. 1996. Therapeutic values onions (Allium cepa L.) and garlic (Allium sativum L). Indian J. Exp. Biol. 34, 634-640.
  2. Basra, A. S., Singh, B. and Malik, C. P. 1994. Amelioration of the effects of ageing in onion seed by osmotic priming and associated changes in oxidative metabolism. Biologia. Plantarum. 36, 365-371. https://doi.org/10.1007/BF02920933
  3. Cha, Y. J., Jeong, E. J., Jeon, S. Y. and Baek, J. H. 2011. Method for preparation of onion process residuum extract having antioxidant and fibrinolysis. Korean patent 10-1101189.
  4. Choi, G. I., Seong, K. Y. and Kim, J. G. 2005. Behaviors of the fungicide procymidone in soils. Korean J. Environ. Agric. 24, 123-131. https://doi.org/10.5338/KJEA.2005.24.2.123
  5. Choi, S. W. and Park, S. Y. 2007. Removal of procymidone by ozonated water. J. Environ. Sci. 16, 1425-1430. https://doi.org/10.5322/JES.2007.16.12.1425
  6. Gyeonggi-do government research insititute of public health & environment. 2012. Risk assessment for pesticide residues of agricultrual products in Gyeonggi-do. pp. 18-20.
  7. Hermann, K. 1976. Flavonoids and flavones in food plants, A review. J. Food Tech. 11, 433-488. https://doi.org/10.1111/j.1365-2621.1976.tb00743.x
  8. Hubbard, G. P., Stevens, J. M., Cicmil, M., Sage, T., Jordan, P. A., Williams, C. M., Lovegrove, J. A. and Gibbins, J. M. 2003. Quercetin inhibits collagen-stimulated platelet activation through inhibition of multiple components of the glycoprotein VI signaling pathway. J. Thromb. Haemost. 1, 1079-1088. https://doi.org/10.1046/j.1538-7836.2003.00212.x
  9. Jeon, S. Y., Baek, J. H., Jeong, E. J. and Cha, Y. J. 2012. Optimal extraction conditions of flavonoids from onion peels via response surface methodology. J. Korean Soc. Food Sci. Nutr. 41, 695-699. https://doi.org/10.3746/jkfn.2012.41.5.695
  10. Jeon, S. Y., Baek, J. H., Jeong, E. J. and Cha, Y. J. 2012. Potential of onion peel extract as a functional ingredient for functional foods. J. Life Sci. 22, 1207-1213. https://doi.org/10.5352/JLS.2012.22.9.1207
  11. Jeon, S. Y., Jeong, E. J., Baek, J. H. and Cha, Y. J. 2011. Analytical method validation of quercetin in Changnyeong onion extract as a functional ingredient for functional health food. J. Korean Soc. Food Sci. Nutr. 40, 565-569. https://doi.org/10.3746/jkfn.2011.40.4.565
  12. Kim, H. K. and Lee, K. S. 2002. Effect of coverings on the growth of ginseng and the persistency of procymidone in growing soil. Korean J. Environ. Agric. 21, 24-30. https://doi.org/10.5338/KJEA.2002.21.1.024
  13. Korea Food & Drug Administration. 2009. Maximum residue levels for pesticides in foods. pp. 272-275.
  14. Korea Food & Drug Administration. 2010. Document registration request form for functional ingredients. p. 42.
  15. Korea Food & Drug Administration. 2012. Pesticide residue database. Available from http://fse.foodnara.go.kr/residue/pesticides/pesticides_info_view.jsp.
  16. Lee, B. G., Sin, D. B. and Ha, S. D. 2005. Removal efficiency of residual pesticides in mini-tomatoes by using CaO (scallop-shell powder). J. Food Hyg. Safety 20, 114-117.
  17. Leighton, T., Ginther, C., Fluss, L., Harter, W. K., Cansado, J. and Nortario, V. 1992. Molecular characterization of quercetin and quercetin glycosides in Allium vegetables. pp. 220, Phenolic Compounds in Food and Their Effects on Health II, ACS, Washington, D.C. USA.
  18. National agricultural products quality management service. 2009. Press Releases: Consumer awareness and attitudes about agricultural products quality management. pp. 1-4.
  19. Ong, K. C., Cash, J. N., Zabik, M. J., Siddiq, M. and Jones, A. L. 1996. Chlorines and ozone washes for pesticide removal from apples and processed apple sauce. Food Chem. 55, 153-160. https://doi.org/10.1016/0308-8146(95)00097-6
  20. Pan, G. X., Bolton, J. L. and Leary, G. J. 1998. Determination of ferulic and p-coumaric acids in wheat straw and the amounts released by mild acid and alkaline peroxide treatment. J. Agric. Food Chem. 46, 5283-5288. https://doi.org/10.1021/jf980608f
  21. Park, J. H., Ahn, K. C. and Lee, J. K. 2000. Removal of air pollutants using photosensitizers/photocatalysts. Korean J. Environ. Agric. 19, 284-293.
  22. Park, Y. K. 1995. Source and processing technology of vegetable juice and the trend of study. Bulletin Food Tech. 8, 59-68.
  23. Rice-Evans, C. A., Miller, N. J., Bolwell, P. G., Bremley, P. M. and Pridham, J. B. 1995. The relative antioxidant activities of plant-derived polyphenolic flavonoids. Free Radic. Res. 22, 375-383. https://doi.org/10.3109/10715769509145649
  24. Shim, A. R., Choi, E. H. and Lee, S. R. 1984. Removal of malathion residues from fruits and vegetables by washing processes. Korean J. Food Sci. Technol. 16, 418-422
  25. Sung, J. M., Kwon, K. H., Kim, J. H. and Jeong, J. W. 2011. Removal efficiency of microorganism and pesticide residues by a using surface washing system on Yuja (Citus junos Sieb ex Tanaka). Korean J. Food Preserv. 18, 627-635. https://doi.org/10.11002/kjfp.2011.18.5.627
  26. Tomlin, C. 1994. The pesticide manual. pp. 254-255, 10th eds., The British Crop Protection Council, U.K.

피인용 문헌

  1. Study on the Synthesis of Isoquercitrin by Enzymatic Partial Hydrolysis vol.25, pp.2, 2014, https://doi.org/10.14478/ace.2013.1119