Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Insulin-like Growth Factor-I Induces Plectin and MACF1 Expression in C2C12 Myotubes

C2C12 myotube에서 insulin-like growth factor-I이 plectin과 MACF1 발현에 미치는 영향

  • Kim, Hye Jin (Department of Exercise Science, College of Health Sciences, Ewha Womans University) ;
  • Hwang, Ji Sun (Department of Exercise Science, College of Health Sciences, Ewha Womans University) ;
  • Kwak, Yi-Sub (Department of Physical Education, College of Sport Science, Dong-Eui University) ;
  • Lee, Won Jun (Department of Exercise Science, College of Health Sciences, Ewha Womans University)
  • 김혜진 (이화여자대학교 건강과학대학 체육과학과) ;
  • 황지선 (이화여자대학교 건강과학대학 체육과학과) ;
  • 곽이섭 (동의대학교 체육과학대학 체육학과) ;
  • 이원준 (이화여자대학교 건강과학대학 체육과학과)
  • Received : 2012.10.19
  • Accepted : 2012.12.05
  • Published : 2012.12.30
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Abstract

Plectin and microtubule actin cross-linking factor 1 (MACF1) are architectural proteins that contribute to the function of skeletal muscle as generators of mechanical force. However, the influence of insulin- like growth factor-I (IGF-I), a master regulator of skeletal muscle cells, on plectin and MACF1 in skeletal muscle cells has not been demonstrated. The effect of IGF-I on plectin and MACF1 gene expression was investigated by treating differentiated C2C12 murine skeletal muscle cells with 20 ng/ml of IGF-I at different time points. The IGF-I treatment increased plectin protein expression in a dose-dependent manner. The mRNA level of plectin was measured by real-time quantitative PCR to determine if plectin induction was regulated pretranslationally. IGF-I treatment resulted in a very rapid induction of plectin mRNA transcript in C2C12 myotubes. Plectin mRNA increased by 140 and 180% after 24 and 48 hours of IGF-I treatment, respectively, and returned to the control level after 72 hours of IGF-I treatment. MACF1 mRNA increased 86 and 90% after 24 and 48 hours of IGF-I treat-ment, respectively, and returned to the control level after 72 hours of IGF-I treatment. These results suggested that the plectin gene is regulated pretranslationally by IGF-I in skeletal muscle cells. In conclusion, IGF-I induces a rapid transcriptional modification of the plectin and MACF1 genes in C2C12 skeletal muscle cells and has modulating effects on a cytolinker protein as well as on contractile proteins.

본 연구에서는 C2C12 근육 세포에서 IGF-I이 세포골격 연결 단백질인 plectin과 MACF1 유전자 발현에 미치는 영향에 대해 알아보았다. 그 결과 IGF-I이 plectin 유전자의 단백질과 mRNA 발현을 증가시켰으며, MACF1 mRNA 발현을 증가시켰음을 알 수 있었다. 이는 운동에 의해 근육에서 분비가 증가하는 IGF-I이 근육 관련 유전자들의 발현을 조절하여 근부피 유지에 영향을 미친다는 기존의 연구 결과들에서 더 나아가 골격근 구조 안정화 및 근수축 기전에 기여하는 plectin과 MACF1 유전자 발현에도 영향을 미친다는 사실을 증명하였다는데 의의가 있다고 사료된다. 향후 근수축 기전에 있어, 운동 형태, 근섬유의 종류에 따른 세포 골격 단백질의 역할 규명 및 조절자에 관한 연구가 더 수행된다면 운동이 골격근의 생리적 변화에 미치는 영향에 대한 추가적 정보를 제공할 수 있을 것이다.

Keywords

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