Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Isolation and antimicrobial susceptibility of microorganisms from milk samples of dairy goat

유산양 유즙으로부터 분리된 세균의 분포 및 항균제 감수성 검사

  • Received : 2012.08.27
  • Accepted : 2012.11.19
  • Published : 2012.12.30
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Abstract

The aim of this study was to isolate microorganisms from half milk samples of dairy goats by California mastitis test (CMT) during the lactation period and to further investigate the susceptibility of isolated organisms to antimicrobial drugs. From a total of 235 half milk samples with CMT scores of 2 or above from 366 dairy goats distributed throughout Jeonnam province, microorganisms were isolated from 198 (83.5%) samples either singly (99.0%) or in combination (1.0%). The most prevalent microorganism was the coagulase-negative Staphylococcus spp., (44.4%, n=88) followed by Staphylococcus aureus (24.2%, n=48), Escherichia coli (11.1%, n=22) and Streptococcus spp. (7.6%, n=15). Isolated bacteria also included Bacillus spp. (2.5%, n=5), Pseudomonas spp. (2.5%, n=5), Micrococcus spp. (1.5%, n=3), Corynebacterium spp. (1.5%, n=3), Enterococcus facium (1.0%, n=2), Morganella morganii (0.5%, n=1) and Streptococcus agalactiae (0.5%, n=1). During the summer season, a high prevalence of all microorganisms were observed in which Staphylococcus spp. (30.8%), Escherichia coli (8.6%), and Streptococcus spp. (5.6%) were among the most prevalent bacteria isolated. Staphylococcus spp. was also shown to be high in the winter (21.7%). In most samples, the presence of bacterial pathogens in goat milk led to the increase in the total somatic cell count (SCC). Most of the half milk samples of dairy goats with bacterial contamination showed SCC of ${\geq}1{\times}10^6cells/ml$ (90.4%). Minor pathogens (11.4%) were more detected from milk samples with SCC of < $1{\times}10^6cells/ml$ than major pathogens (4.1%), while the major pathogens tended to be higher from samples with SCC of ${\geq}3{\times}10^6cells/ml$. Susceptibility of these bacteria to 12 antimicrobial agents was tested by the Kirby-Bauer disc diffusion method. Results indicated that more than 90% of bacteria isolated from CMT 2+ dairy goat half milk samples were susceptible to trimethoprim/sulfamethoxazole, amoxicillin/clavulanic, enrofloxacin and cephalothin while they were resistant to tetracycline (44.7%).

Keywords

References

  1. Albenzio M, Caroprese M, Marino R, Muscio A, Santillo A, Sevi A. 2006. Characteristics of Garganica goat milk and Cacioricotta cheese. Small Ruminant Res 64: 35-44. https://doi.org/10.1016/j.smallrumres.2005.03.010
  2. Ameh JA, Tari IS. 1999. Observations on the prevalence of caprine mastitis in relation to predisposing factors in Maiduguri. Small Ruminant Res 35: 1-5. https://doi.org/10.1016/S0921-4488(99)00047-4
  3. Bagnicka E, Winnicka A, Jóźwik A, Rzewuska M, Strzałkowska N, Kościuczuk E. Prusaka B, Kaba J, Horbańczuk J, Krzyżewskia J. 2011. Relationship between somatic cell count and bacterial pathogens in goat milk. Small Ruminant Res 100: 72-77. https://doi.org/10.1016/j.smallrumres.2011.04.014
  4. Bauer AW, Kirby WM, Sherris JC, Turck M. 1966. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 45: 493-496. https://doi.org/10.1093/ajcp/45.4_ts.493
  5. Bergonier D, de Crémoux R, Lagriffoul G, Berthelot X. 2003. Mastitis of dairy small ruminants. Vet Rec 34: 689-716. https://doi.org/10.1051/vetres:2003030
  6. Contreras A, Corrales JC, Sierra D. Marco J. 1995. Prevalence and aetiology of non-clinical intramammary infection in Muriciano-Granadia goats. Small Ruminant Res 17: 71-78. https://doi.org/10.1016/0921-4488(95)00651-Z
  7. Contreras A, Luengo C, Sánchez A, Corrales JC. 2003. The role of intamammary pathogens in dairy goats. Livest Prod Sci 79: 273-283. https://doi.org/10.1016/S0301-6226(02)00172-0
  8. Contreras A, Paape MJ, Miller RH. 1999. Prevalence of subclinical intramammary infection caused by Staphylococcus epidermidis in a commercial dairy goat herd. Small Ruminant Res 31: 203-208. https://doi.org/10.1016/S0921-4488(98)00147-3
  9. Delgado-Pertinez M, Alcalde MJ, Guzman-Guerrero, JL, Castel JM, Mena Y, Caravaca F. 2003. Effect of hygiene-sanitary management on goat milk quality in semi-extensive system in Spain. Small Ruminant Res 47: 51-61. https://doi.org/10.1016/S0921-4488(02)00239-0
  10. Gonzalez RN, Jasper DE, Kronlund NC, Farver TB, Cullor JS, Bushnell RB, Dellinger JD. 1990. Clinical mastitis in two California dairy herds participating in contagious mastitis control programs. J Dairy Sci 73: 648-660. https://doi.org/10.3168/jds.S0022-0302(90)78716-4
  11. Haenlein GFW. 2002. Relationship of somatic cell counts in goat milk to mastitis and productivity. Small Ruminant Res 45: 163-178. https://doi.org/10.1016/S0921-4488(02)00097-4
  12. Hall SM, Rycroft AN. 2007. Causative organisms and somatic cell counts in subclinical intramammary infections in milking goats in the UK. Vet Rec 160: 19-22. https://doi.org/10.1136/vr.160.1.19
  13. Harmon RJ. 1994. Physiology of mastitis and factors affecting somatic cell counts. J Dairy Sci 77: 2103-2112. https://doi.org/10.3168/jds.S0022-0302(94)77153-8
  14. Kalogridou-Vassiliadou D, Manolkidis KH. 1991. Changes in mastitis pathogens in goat milk throughout lactation. Small Ruminant Res 4: 197-20. https://doi.org/10.1016/0921-4488(91)90065-X
  15. Kang JH, Kim SD, Park JY, Cho IY, Hur TY, Jun YH, Choi CY, Jung JY, Shin SS, Son CH, Ok KS, Suh GH. 2011. A retrospective study of the incidence of clinical mastitis found in large-scale dairy herds in Korea. Korean J Vet Serv 34: 369-378. https://doi.org/10.7853/kjvs.2011.34.4.369
  16. Kim HR, Lee JC, Jung JY, Lee YK, Shin SS, Lee CY. 2007. A study on the somatic cells in half milk samples of dairy goats. J Vet Clin 24: 177-181.
  17. Kim ST, Kim SD, Park JY, Cho IY, Jung JY, Lee JD, Hur TY, Jung YH, Choi CY, Shin SS, Son CH, Ok KS, Suh GH. 2011. Isolation and antimicrobial susceptibility of microorganisms from milk samples of healthy postpartum sows. Korean J Vet Serv 34: 361-368. https://doi.org/10.7853/kjvs.2011.34.4.361
  18. Lafi SQ. 2006. Use of somatic cell counts and California mastitis test results from udder halves milk samples to detect subclinical intramammary infection in Awassi sheep. Small Ruminant Res 62: 83-86. https://doi.org/10.1016/j.smallrumres.2005.07.035
  19. Lee JC, Lee CY, Kim SK, Lee CG, Suh GH. 2003. Antimicrobial susceptibility of microorganisms isolated from quarter milk samples of Holstein cows. J Vet Clin 20: 166-171.
  20. Lee JD, Lee YK, O SI, Jung JY, Son CH, Shin SS, Ok KS, Hur TY, Suh GH. 2011. Isolation and antimicrobial susceptibility of microorganisms from milk samples of Jindo dogs (Canis familiaris var. jindo). Korean J Vet Res 51: 29-35.
  21. Leitner G, Merin U, Silanikove N, Ezra E, Chaffer M, Gollop N, Winkler M, Glickman A, Saran A. 2004. Effect of subclinical intramammary infection on somatic cell counts, NAGase activity and gross composition of goats' milk. J Dairy Res 71: 31-315.
  22. Leitner G, Merin U, Lavi Y, Egber A, Silanikove N. 2007. Aetiology of intramammary infection and its effect on milk composition in goat flocks. J Dairy Res 74: 186-193. https://doi.org/10.1017/S0022029906002299
  23. Malher X, Seegers H, Beaudeau F. 2001. Culling and mortality in large dairy goat herds managed under intensive conditions in western France. Livert Prod Sci 71: 75-86. https://doi.org/10.1016/S0301-6226(01)00242-1
  24. Marogna G, Pilo C, Vidili A, Tola S, Schianchi G, Leori SG. 2012. Comparison of clinical findings, microbiological results, and farming parameters in goat herds affected by recurrent infectious mastitis. Small Ruminant Res 102: 74-83. https://doi.org/10.1016/j.smallrumres.2011.08.013
  25. McDougall S, Pankey W, Delaney C, Barlow J, Murdough PA, Scruton D. 2002. Prevalence and incidence of subclinical mastitis in goats and dairy ewes in Vermont, USA. Small Ruminant Res 46: 115-121. https://doi.org/10.1016/S0921-4488(02)00191-8
  26. McDougall S, Voermans M. 2002. Influence of estrus on somatic cell count in dairy goats. J Dairy Res 85: 378-383. https://doi.org/10.3168/jds.S0022-0302(02)74084-8
  27. Min BR, Tomita G, Hart SP. 2007. Effect of subclinical intramammary infection on somatic cell counts and chemical composition of goats' milk. J Dairy Res 74: 204-210. https://doi.org/10.1017/S0022029906002378
  28. Mitchell JM, Griffiths MW, McEwen SA, McNab WB, Yee AJ. 1998. Antimicrobial drug residues in milk and meat: causes, concerns, prevalence, regulations, tests and test performance. J Food Prot 61: 742-756. https://doi.org/10.4315/0362-028X-61.6.742
  29. Moroni P, Pisoni G, Ruffo G, Boettcher PJ. 2005a. Risk factors for intramammary infections and relationship with somatic- cell counts in Italian dairy goats. Prev Vet Med 69: 163-173. https://doi.org/10.1016/j.prevetmed.2004.10.013
  30. Moroni P, Pisoni G, Vimercati C, Rinaldi M, Castiglioni B, Cremonesi P, Boettcher P. 2005b. Characterization of Staphylococcus aureus isolated from chronically infected dairy goats. J Dairy Sci 88: 3500-3509. https://doi.org/10.3168/jds.S0022-0302(05)73035-6
  31. Nam HM, Lim SK, Kim JM, Jang GC, Jung SC, Wee SH, Han HR, Lee CS. 2011. Somatic cell counts and bacterial status in udder half milk samples of lactating goats in Korea during 2008. Kor J Vet Publ Hlth 35: 61-66.
  32. Paape MJ, Wiggans GR, Bannerman DD. Thomas DL. Sanders AH, Contreras A, Moroni P, Miller RH. 2007. Monitoring goat and sheep milk somatic cell counts. Small Ruminant Res 68: 114-125. https://doi.org/10.1016/j.smallrumres.2006.09.014
  33. Sanchez A, Sierra D, Luengo C, Corrales JC, Morales CT, Contreras A, Gonzalo C. 2005. Influence of storage and preservation on Fossomatic cell count and composition of goat milk. J Dairy Sci 88: 3095-3100. https://doi.org/10.3168/jds.S0022-0302(05)72991-X
  34. White EC, Hinckley LS. 1999. Prevalence of mastitis pathogens in goat milk. Small Ruminant Res 33: 117-121. https://doi.org/10.1016/S0921-4488(99)00013-9
  35. Yoon JC, Lee JC, Kim SK, Park YS, Kim JT, Lee CG, Lee CY. 2004. Prevalence of isolated microorganisms and antimicrobial susceptibility from half milk in dairy goats. Korean J Vet Res 44: 151-157.

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