한국동물위생학회지 (Korean Journal of Veterinary Service)

  • 제35권4호
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  • Pages.289-294
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  • 2012
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  • 3022-7372(pISSN)
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  • 2287-7630(eISSN)
한국동물위생학회 (The Korean Society of Veterinary Service)
권호 표지
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A newly developed consensus polymerase chain reaction to detect Mycoplasma species using 16S ribosomal RNA gene

  • Hong, Sunhwa (Center for Animal Resource Development, Wonkwang University) ;
  • Park, Sang-Ho (Korea DNA Valley Co. Ltd.) ;
  • Chung, Yung-Ho (Department of Companion Animal and Animal Resources Science, Joongbu University) ;
  • Kim, Okjin (Center for Animal Resource Development, Wonkwang University)
  • 투고 : 2012.10.22
  • 심사 : 2012.11.29
  • 발행 : 2012.12.30
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초록

Mycoplasmas are highly fastidious bacteria, difficult to culture and slow growing. Infections with Mycoplasma species can cause a variety of problems in living organisms and in vitro cell cultures. In this study, we investigated the usefulness of a genus-specific consensus PCR analysis method to detect Mycoplasma species. The developed consensus primer pairs MycoF and MycoR were designed specifically to amplify the 16S ribosomal RNA gene (rRNA) of Mycoplasma species by the optimized PCR system. The developed consensus PCR system effectively amplified 215 bp of Mycoplasma genus-specific region of 16S rRNA. In conclusion, we recommend this consensus PCR for monitoring Mycoplasma species in animals, human and cell culture system.

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참고문헌

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