Journal of Sasang Constitutional Medicine (사상체질의학회지)

The Society Of Sasang Constitutional Medicine (사상체질의학회)
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Effects of Yuldahanso-tang and Chungsimyonja-tang on Cytochrome P450 Activities

열다한소탕과 청심연자탕의 Cytochrome P450 활성 연구

  • Jin, Seong-Eun (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Ha, Hye-Kyung (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Shin, Hyeun-Kyoo (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine)
  • 진성은 (한국한의학연구원 한약기초연구그룹) ;
  • 하혜경 (한국한의학연구원 한약기초연구그룹) ;
  • 신현규 (한국한의학연구원 한약기초연구그룹)
  • Received : 2012.10.08
  • Accepted : 2012.12.06
  • Published : 2012.12.31
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Abstract

Objectives : The purpose of this study is to investigate the inhibitory or inductive potentials of Yuldahanso-tang (YDT) and Chungsimyonja-tang (CST), herbal formulas for Taeeumin, on cytochrome P450 (CYP450) drug metabolizing enzyme. The mechanisms for the herbal formula-drug interaction has not been well reported in spite of the chance for co-administration with conventional drugs. Methods : To evaluate the interaction potential of YDT-drug or CST-drug, the fluorescence-based enzyme assays on CYP450 isozymes including CYP3A4, CYP2C19, CYP2D6 and CYP2E1 were established in vitro. The inhibitory effects of herbal formulas were characterized with $IC_{50}$ values. Results : YDT showed inhibitory effects on CYP2D6 and CYP2E1-mediated metabolism, while it exhibited week inhibition on CYP3A4 and CYP2C19 relatively. CST exerted relatively weak inhibitory effects on the four CYP450 isozymes compared to that of YDT. Conclusions : These results suggest that the herbal formula-drug interaction could be occur when YDT are co-administered with drugs mediated by CYP2D6 or CYP2E1.

Keywords

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