Journal of Species Research

  • 제1권2호
  • /
  • Pages.224-231
  • /
  • 2012
  • /
  • 2234-7909(pISSN)
  • /
  • 2713-8615(eISSN)
국립생물자원관 (The National Institute of Biological Resources)
권호 표지
DOI QR코드

DOI QR Code

Genetic diversity assessment of Aconitum coreanum (H. Lév.) Rapaics (Ranunculaceae), an endangered plant species in Korea, using microsatellite markers

  • 투고 : 2012.07.16
  • 심사 : 2012.08.24
  • 발행 : 2012.08.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

To assess the genetic diversity of Aconitum coreanum (Ranunculaceae) populations in Korea, we have amplified and sequenced eight organellar marker regions, and developed and analyzed microsatellite markers. No sequence variation was detected from the eight organellar markers. Ten microsatellites were developed using Next Generation Sequencing and two microsatellite markers, AK_CA03 and AK_CT07, were identified polymorphic and applied for 143 individuals of twelve A. coreanum populations. Four and five alleles were detected for the two microsatellite loci, respectively, and number of migrants ($N_m$) was estimated as 1.12586. Two microsatellite marker loci showed $F_{ST}$ of 0.205 and 0.275, respectively. The heterozygosity deficit, low level of among-population differentiation, small size of gene flow, and lack of sequence variation of the organellar markers suggest that A. coreanum is reproductively isolated from other Aconitum species and there has been continuous gene flow among the populations of A. coreanum or it has dispersed relatively recently after speciation. Though population pairwise $F_{ST}$'s presented significant geographic structure, further sampling and study will be necessary to confirm this.

키워드

참고문헌

  1. An, H.S., J.-h. Lee, J.K. Noh, H.C. Kim, C.J. Park, B.H. Min and J.-I. Myeong. 2010. Ten new microsatellite markers in cutlassfish Trichiurus lepturus derived from an enriched genomic library. Anim. Cells. Syst. 14:169-174. https://doi.org/10.1080/19768354.2010.504347
  2. Bessonova, I.A., M.S. Yunusov, V.G. Kondrat'ev and A.I. Shreter. 1987. Alkaloids of Aconitum coreanum. I. Structure of acorine. Chemistry of Natural Compounds 23:573-575. https://doi.org/10.1007/BF00598676
  3. Bosch, M. and N.M. Waser. 1999. Effects of local density on pollination and reproduction in Delphinium nuttallianum and Aconitum columbianum (Ranunculaceae). Amer. J. Bot. 86:871-879. https://doi.org/10.2307/2656707
  4. Chung, K.-S., B. Nam, M.S. Park, J.A. Eom, B.-U. Oh and G.Y. Chung. 2011. Chromosome numbers of eight taxa of Aconitum L. in Korea and their systematic significance (Ranunculaceae). Korean. J. Pl. Taxon. 41:215-222.
  5. Chung, M.G. and C.-W. Park. 2000. Notes on spatial genetic structure in a hybrid population between Aconitum japonicum subsp. napiforme and A. jaluense (Ranunculaceae). Ann. Bot. Fenn. 37:243-247.
  6. Dakin, E.E. and J.C. Avise. 2004. Microsatellite null alleles in parentage analysis. Heredity 93:504-509. https://doi.org/10.1038/sj.hdy.6800545
  7. Demesure, B., N. Sodzi and R.J. Petit. 1995. A set of universal primers for amplification of polymorphic non-coding regions of mitochondrial and chloroplast DNA in plants. Mol. Ecol. 4:129.131.
  8. Dzhakhangirov, F.N. and I.A. Bessonova. 2002. Alkaloids of Aconitum coreanum. X. Curare-like activity-structure relationship. Chemistry of Natural Compounds 38:74-77. https://doi.org/10.1023/A:1015742001422
  9. Excoffier, L., G. Laval and S. Schneider. 2005. Arlequin ver. 3.0: An integrated software package for population genetics data analysis. Evolutionary Bioinformatics Online 1:47-50.
  10. Fazekas, A.J., K.S. Burgess, P.R. Kesanakurti, S.W. Graham, S.G. Newmaster, B.C. Husband, D.M. Percy, M. Hajibabaei and S.C. H. Barrett. 2008. Multiple multilocus DNA barcodes from the plastid genome discriminate plant species equally well. Public Library of Science ONE 3: e2802. doi:10.1371/journal.pone.0002802.
  11. Glaubitz, J.C. 2004. CONVERT: a user friendly program to reformat diploid genotypic data for commonly used population genetic software packages. Molecular Ecology Notes 4:309-310. https://doi.org/10.1111/j.1471-8286.2004.00597.x
  12. Goudet, J. 2001. FSTAT, a program to estimate and test gene diversities and fixation indices (version 2.9.3). http://www.unil.ch/izea/softwares/fstat.html.
  13. Gupta, P.K., H.S. Balyan, P.C. Sharma, B. Ramesh. 1996. Microsatellites in plants: a new class of molecular markers. Curr. Sci. 70:45-54.
  14. Hamilton, M.B., E.L. Pincus, A. Di Fiore and R.C. Fleischer. 1999. Universal linker and ligation procedures for construction of genomic DNA libraries enriched for microsatellites. BioTechniques 27:500-507.
  15. Jarne, P. and P.J. LLagoda. 1996. Microsatellites, from molecules to populations and back. Trends Ecol. Evol. 11:424-429. https://doi.org/10.1016/0169-5347(96)10049-5
  16. Jin, W., C. Chen, and E.B. Wang. 1998. A karyotaxonomical study on 6 species of Aconitum in Liaoning area. Bull. Bot. Res., Harbin 18:163-172 (in Chinese).
  17. Kadota, Y. 1987. A revision of Aconitum subgenus Aconitum (Ranunculaceae) of East Asia. Sanwa Shoyaku, Utsunomiya.
  18. Kalia, R.K., M.K. Rai, S. Kalia, R. Singh, and A.K. Dhawan. 2011. Microsatellite markers: An overview of the recent progress in plants. Euphytica 177:309-334. https://doi.org/10.1007/s10681-010-0286-9
  19. Lee, H.-W., H.-R. Chung, T.-H. Roh, Y.-H. Kwon, C.-H. Kim, J.-O. Hyun and I.-S. Jang. 2005. Categorization and Conservation of the Threatened Plant Species in Environmental Impact Assessment. Korea Environment Institute (in Korean).
  20. Lee, Y.N. 1967. Chromosome numbers of flowering plants in Korea. J. Korean Res. Inst. Ewha Women's Univ. 11: 455-478.
  21. Li, L. and Y. Kadota. 2001. Aconitum. In: Flora of China Editorial Committee (eds.), Flora of China, vol. 6, Caryophyllaceae through Lardizabalaceae. Missouri Botanical Garden Press, Missouri. pp. 150-227.
  22. Lim, C.E. 2003. Chloroplast DNA Sequence Variation of Aconitum L. subgenus Aconitum (Ranunculaceae) in Korea: Interspecific Relationships and Hybridization. Ph. D. thesis, Seoul National University, Seoul.
  23. Liang, M., S. Li, B. Shen, J. Cai, C. Li, Z. Wang, X. Li, J. Gao, H. Huang, X. Zhang and J. Li. 2012. Anti-hepatocarcinoma effects of Aconitum coreanum polysaccharides. Carbohydrate Polymers 88:973-976. https://doi.org/10.1016/j.carbpol.2012.01.050
  24. Noh, T.-K. 2009. Hybridization in Aconitum subgenus Aconitum (Ranunculaceae) at Mt. Jiri in Korea. Ph.D. Dissertation. Seoul National University (in Korean).
  25. Noh, T.-K. and C.-W. Park. 2000. ITS sequence variation of Korean Aconitum (Ranunculaceae) species. The 55th annual meeting of the Korean Association of Biological Sciences. P. 100 (in Korean).
  26. Oh, S. and C.-W. Park. 1998. Crossability of the Aconitum jaluense species complex (Ranunculaceae) in Korea. Korean J. Biol. Sci. 2:435-438.
  27. Park, C.W. 2007. Aconitum. In: Flora of Korea Editorial Committee (eds.), The Genera of Vascular Plants of Korea. Academy Publishing Co., Seoul, Korea. pp. 176-182.
  28. Raymond M. and F. Rousset. 1995. GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism. J. Heredity 86:248-249 https://doi.org/10.1093/oxfordjournals.jhered.a111573
  29. Rozen, S. and H.J. Skaletsky. 1998. Primer 3. Code available at http://www.genome.wi.mit.edu/genome_software/other/primer3.html.
  30. Schuelke, M. 2000. An economic method for the fluorescent labeling of PCR fragments. Nat. Biotechnol. 18:233-234. https://doi.org/10.1038/72708
  31. Shanker, A., A. Bhargava, R. Bajpai, S. Singh, S. Srivastava and V. Sharma. 2007. Bioinformatically mined simple sequence repeats in UniGene of Citrus sinensis. Sci Hortic-Amsterdam 113:353-361. https://doi.org/10.1016/j.scienta.2007.04.011
  32. Swofford, D.L. 2003. $PAUP^{\ast}$. Phylogenetic Analysis Using Parsimony ($^{\ast}$ and Other Methods). Version 4. Sinauer Associates, Sunderland, Massachusetts.
  33. Taberlet, P., L. Gielly, G. Patou and J. Bouvet. 1991. Universal primers for amplification of three noncoding regions of chloroplast DNA. Pl. Mol. Biol. 17:1105-1109. https://doi.org/10.1007/BF00037152
  34. Tang, Q.-Fa., W.-C. Ye, J.-H. Liu and C.-H. Yang. 2012. Three new hetisine-type diterpenoid alkaloids from Aconitum coreanum. Phytochemistry Letters 5:397-400. https://doi.org/10.1016/j.phytol.2012.03.011
  35. Tehrani, M.S., M.M. Mardi, J. Sahebi, P. Catalan and A. Diaz-Perez. 2009. Genetic diversity and structure among Iranian tall fescue populations based on genomic-SSR and EST-SSR marker analysis. Plant. Syst. Evol. 282:57-70. https://doi.org/10.1007/s00606-009-0207-3
  36. Van Oosterhout, C., W.F. Hutchinson, D.P.M. Wills and P. Shipley. 2004 MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite. Mol Ecol Notes 4:535-538 https://doi.org/10.1111/j.1471-8286.2004.00684.x
  37. Wang, Y., F. Wang, Y.H. Shi, Z.F. Gu and A.M. Wang. 2011. Development and characterization of 60 microsatellite markers in the abalone Haliotis diversicolor. Genet. Mol. Res. 10:860-866. https://doi.org/10.4238/vol10-2gmr1182
  38. Won, H. and S.S. Renner. 2005. The internal transcribed spacer of nuclear ribosomal DNA in the gymnosperm Gnetum. Mol. Phylogen. Evol. 36:581-597. https://doi.org/10.1016/j.ympev.2005.03.011
  39. Won, H. 2009. Phylogenetic position of Corchoropsis Siebold & Zucc. (Malvaceae s.l.) inferred from plastid DNA sequences. J. Plant Biol. 52:411-416. https://doi.org/10.1007/s12374-009-9052-8
  40. Yu, J.-N., C. Won, J. Jun, Y. Lim and M. Kwak. 2011. Fast and cost-effective mining of microsatellite markers using NGS technology: An example of a Korean water deer Hydropotes inermis argyropus. PLoS ONE 6(11):e26933. https://doi.org/10.1371/journal.pone.0026933
  41. Zeng, L., T.-R. Kwon, X. Liu, C. Wilson, C.M. Grieve and G.B. Gregorio. 2004. Genetic diversity analyzed by microsatellite markers among rice (Oryza sativa L.) genotypes with different adaptations to saline soils. Plant Sci. 166:1275-1285. https://doi.org/10.1016/j.plantsci.2004.01.005