한국결정성장학회지 (Journal of the Korean Crystal Growth and Crystal Technology)

  • 제22권6호
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  • Pages.279-285
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  • 2012
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  • 1225-1429(pISSN)
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  • 2234-5078(eISSN)
한국결정성장학회 (The Korea Association of Crystal Growth)
권호 표지
DOI QR코드

DOI QR Code

골결손부 재건을 위한 금속 이온 치환 이상인산칼슘 합성 및 생체 활성 평가

Synthesis and bioactivity evaluation of metal ion-substitution biphasic calcium phosphate for bone defect reconstruction

  • 김태완 (부산대학교 재료공학부) ;
  • 김동현 (부산대학교 재료공학부) ;
  • 진형호 (부산대학교 재료공학부) ;
  • 이승호 (한국세라믹기술원 에코복합소재센터) ;
  • 박홍채 (부산대학교 재료공학부) ;
  • 윤석영 (부산대학교 재료공학부)
  • Kim, Tae-Wan (School of Materials Science Engineering, Pusan National University) ;
  • Kim, Dong-Hyun (School of Materials Science Engineering, Pusan National University) ;
  • Jin, Hyeong-Ho (School of Materials Science Engineering, Pusan National University) ;
  • Lee, Seung Ho (Echo Composite Materials Center, Korea Institute of Ceramic Engineering and Technology) ;
  • Park, Hong-Chae (School of Materials Science Engineering, Pusan National University) ;
  • Yoon, Seog-Young (School of Materials Science Engineering, Pusan National University)
  • 투고 : 2012.10.08
  • 심사 : 2012.11.23
  • 발행 : 2012.12.31
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초록

BCP, Mg-BCP, Si-BCP 분말을 공침법(co-precipitation process)을 이용하여 합성하였다. 제조된 분말을 X-선 회절 분석(XRD), 적외선분광분석(FTIR)을 이용하여 특성을 분석하였다. Ca/P 몰 비율이 1.602인 칼슘 결손 아파타이트 공침물을 제조하여 $1000^{\circ}C$ 열처리 과정을 통하여 HAp와 ${\beta}$-TCP 상이 혼재된 BCP, Mg-BCP, Si-BCP을 합성할 수 있었다. 제조된 분말의 생체활성 거동을 평가하기 위하여 Hanks' Balanced Salt Solution(HBSS)에 침적시켜 시간에 따라 형상의 변화 및 결정상을 분석한 결과, BCP 분말에 비하여 금속 이온이 치환된 BCP 분말에서 빠른 생체활성을 관찰할 수 있었다. MTT assay를 통한 세포 성장률 평가에서모든 분말에서 시간 경과에 따라 독성을 나타내지 않았으며, 세포의 활성이 증가하였다.

The co-precipitation technique has been applied to synthesize Biphasic Calcium Phosphate (BCP), Mg-BCP and Si-BCP. X-ray diffraction (XRD) and Fourier transformed infrared (FTIR) spectroscopy were used to characterize the structure of synthesized BCP, Mg-BCP and Si-BCP powders. The results have shown that BCP and substitution of magnesium and silicon in the calcium deficient apatites revealed the formation of biphasic mixtures of Hydroxyapatite (HAp)/${\beta}$-Tricalcium phosphate (${\beta}$-TCP) ratios after heating at $1000^{\circ}C$. Ionic substituted BCP is able to develop a new apatite phase on the surface in contact with physiological fluids faster than BCP does. An MTT assay indicated that BCP, Mg-BCP, and Si-BCP powders had no cytotoxic effects on MG-63 cells, and that they have good biocompatibility.

키워드

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