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오가피순 추출물의 항산화 효과 및 아질산염 소거능

Antioxidant Effects and Nitrite Scavenging Ability of Extract from Acanthopanax cortex Shoot

  • Yu, Seok-Yeong (Dept. of Food Science and Biotechnology, Kangwon National University) ;
  • Lee, Young-Jun (Dept. of Food Science and Biotechnology, Kangwon National University) ;
  • Song, Ho-Seong (Chamjoa Food) ;
  • Hong, Hee-Do (Korea Food Research Institute) ;
  • Lim, Jeong-Ho (Korea Food Research Institute) ;
  • Choi, Hyeon-Son (Dept. of Food Science and Biotechnology, CHA University) ;
  • Lee, Boo-Yong (Dept. of Food Science and Biotechnology, CHA University) ;
  • Kang, Suk-Nam (Dept. of Animal Resources Technology, Gyeongnam National University of Science and Technology) ;
  • Lee, Ok-Hwan (Dept. of Food Science and Biotechnology, Kangwon National University)
  • 투고 : 2012.10.03
  • 심사 : 2012.11.05
  • 발행 : 2012.12.31

초록

본 연구에서는 오가피순 추출물의 항산화 효과를 평가하였다. 오가피순 추출물의 총 페놀 함량과 플라보노이드 함량은 각각 $116.33{\pm}6.09mg\;GAE/g$, $65.07{\pm}4.10mg\;RE/g$으로 나타났다. 오가피순 추출물의 항산화능은 다양한 항산화 모델(DPPH, FRAP, Reducing power, ABTS, ORAC assay)을 이용하여 평가하였다. ORAC assay를 제외한 위의 항산화 모델에서는 0.1, 0.5 및 $1.0mg/m{\ell}$의 농도로 측정한 결과, DPPH 라디칼 소거능은 오가피순 추출물의 농도가 증가함에 따라 $23.3{\pm}0.8$, $28.8{\pm}1.3$$33.7{\pm}3.7%$로 평가되었고, ABTS 경우에는 6.42, 12.47 및 16.69%로 측정되었다. 또한, FRAP 활성은 0.081, 0.160 및 0.259로, Reducing power는 0.034, 0.097 및 0.180으로 나타나 추출물의 농도가 증가함에 따라 항산화활성도 증가하였다. 한편 $0.1mg/m{\ell}$에서의 ORAC value는 $103.4{\pm}3.6{\mu}M\;TE/g$으로 나타났고, 아질산염 소거능은 0.1, 0.5 및 $1.0mg/m{\ell}$의 농도에서 각각 3.6, 9.0 및 13.2%로 나타났다. 최근 건강기능 식품의 관심도가 증가하는 것을 고려해 볼 때, 오가피순 추출물은 기능성식품 또는 천연 항산화제의 소재로써의 이용 가능성을 보였다.

This study was conducted to examine the antioxidative effect and nitrite scavenging ability of extract from Acanthopanax cortex shoot. The total phenolic compound and flavonoids contents of extract from Acanthopanax corex shoot were $116.33{\pm}6.09mg\;GAE/g$ and $65.07{\pm}4.10mg\;RE/g$, respectively. Antioxidative activities were measured by various in vitro models such as DPPH radical scavenging activity, FRAP, reducing power, ABTS radical scavenging activity, ORAC assay. This results showed that the extract of Acanthopanax cortex shoot was effective in scavenging radicals and protecting oxidation when assessed various in vitro systems. Similarly, the nitrite scavenging ability of extract was increased in a dose-dependent manner. Moreover, ORAC value at a concentration of $0.1mg/m{\ell}$ was $103.4{\pm}5.6{\mu}M\;TE/g$. Considering high consumer demand beneficial health effects, Acanthopanax cortex shoot can be utilized to develop functional food health-promoting and natural antioxidant agents.

키워드

참고문헌

  1. Benzie I, Stranin J. 1996. The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: The FRAP assay. Anal Biochem 239:70-76 https://doi.org/10.1006/abio.1996.0292
  2. Cho YS. 2006. Evaluation of phytonutrients and antioxidant activities of Korean vegetables, fruits and legumes. MS Thesis, Yonsei Uni. Seoul. Korea
  3. Choi JM, Kim KY, Lee SH, Ahn JB. 2011. Functional properties of water extracts from different parts of Acanthopanax sessiliflorus. Food Engineering Progress 15:130-135
  4. Chung SY, Kim NK, Yoon S. 1999. Nitrite scavenging effect of methanol fraction obtained from green yellow vegetable juices. J Korean Soc Food Sci Nutr 28:342-347
  5. Gray JI, Dugan JRL. 1975 Inhibition of N-nitrosamine formation in model foor system. J Food Sci 40:981-985 https://doi.org/10.1111/j.1365-2621.1975.tb02248.x
  6. Gutfinger T. 1981. Polyphenols in olive oils. J Am Oil Chem Soc 58:966-967 https://doi.org/10.1007/BF02659771
  7. Hahn DR, Kim CJ, Kim JH. 1985. A study on chemical constituents of Acanthopanax koreanum Nakai and its pharmacobiological activities. Yakhak Hoeji 29:357-361
  8. Ham SH, Lim BL, Yu JH, Ka SO, Park BH. 2008. Fermentation increases antidiabetic effects of Acanthopanax senticosus. Korean J Oriental Physiology & Pathology 22:340-345
  9. Han YS. 2002. A study on the effect of antiinflammatory plant extracts on melanogenesis. Ph.D. Thesis. Ajou Uni. Suwon. Korea
  10. Heinemann T, Axtmann G, Von Bergmann K. 1993. Comparison of intestinal absorption of cholesterol with different plant sterols in man. Eur J Clin Invest 23:827-831 https://doi.org/10.1111/j.1365-2362.1993.tb00737.x
  11. Heo SJ, Ahn HY, Kang MJ, Lee JH, Cha JY and Cho YS. 2011. Antioxidative activity and chemical characteristics of leaves, roots, stems and fruits extracts from Acanthopanax senticosus. Journal of Life Science 21:1052-1059 https://doi.org/10.5352/JLS.2011.21.7.1052
  12. Kim JH, Park JH, Park SD, Choi SY, Seong JH, Hoon KD. 2002. Preparation and antioxidant activity of health drink with extract powders from safflower (Carthamus tinctorius L.) seed. Korea J Food Sci 34:617-624
  13. Kim YH, Park YS. 2006. Effect of Acanthopanacis cortex water extract on antioxidative activity, lipid profile and epidermal thickness in DNCB-induced allergic contract dermatitis animal model. Korean J Food Sci Technol 38:668-673
  14. Li H, Choi YM, Lee JS, Park JS, Yeon KS, Han CD. 2007. Drying and antioxidant characteristics of the shiitake (Lentinus edodes) mushroom in a conveyer-type far-infrared dryer. J Korean Soc Food Sci Nutr 36:250-254 https://doi.org/10.3746/jkfn.2007.36.2.250
  15. Lim SY, Leem JY, Lee CS, Jang YJ, Park JW, Yoon S. 2007. Antioxidant and cell proliferation effects of Acanthopanax senticosus extract in human osteoblast-like mg-63 cell line. Korean J Food Sci Technol 39:694-700
  16. Mok JY, Kang HJ, Cho JK, Jeon IH, Kim HS, Park JM, Jeong SI, Shim JS, Jang SI. 2011. Antioxidative and antiinflammatory effects of extracts from different organs of Cirsium japonicum var. ussuriense. Kor J Herbology 26:39-47
  17. Moreno MIN, Isla MIN, Sampietro AR, Vattuone MA. 2000. Comparison of the free radical scavenging activity of propolis from several region of Argentina. Journal of Enthropharmacology 71:109-114 https://doi.org/10.1016/S0378-8741(99)00189-0
  18. Ou B, Hampsch-Woodill M, Prior RL. 2001. Development and validation of an improved oxygen radical absorbance capacity assay using fluorescein as the fluorescent probe. J Agric Food Chem 49:4619-4626 https://doi.org/10.1021/jf010586o
  19. Oyaizu M. 1986. Studies on products of the browning reaction: Antioxidative activities of browning reaction products prepared from glucosamine. Jpn J Nutr 44:307-315 https://doi.org/10.5264/eiyogakuzashi.44.307
  20. Park YS. 2010. Antioxidant effects and improvement of lipid metabolism of Acanthopanacis cortex water extract in rats fed high fat diet. J East Asian Soc Dietary Life 20:37-45
  21. Que F, Mao L, Zhu C, Xie G. 2006. Antioxidant properties of Chineses yellow wine, its concentrate and volatiles. LWT-Food Sci Technol 39:111-117 https://doi.org/10.1016/j.lwt.2005.01.001
  22. Roberta R, Nicoletta P, Anna P, Anath P, Min Y, Catherine RE. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Rad Biol Med 26:1231-1237 https://doi.org/10.1016/S0891-5849(98)00315-3
  23. Yoo MY, Kim SK, Yang JY. 2004. Characterization of an antioxidant from sporophyll of Undaria pinnatifida. Kor J Microbiol Biotechnol 32:307-311

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