Abstract
The residue levels of ampicillin (AM) in cultured olive flounder, Paralichthys olivaceus (average 300g) at $20{\pm}1.0^{\circ}C$ were studied by oral, intramuscular and dipping administration (routes). The concentrations of AM in the plasma were determined by HPLC-UV detector. The average recoveries of AM in spiked samples between 0.01~10 ppm were ranging from 84.45% to 91.26% for plasma. The limit of detection for AM was 0.05 ppm by using this method. Plasma concentrations of AM were determined after oral dosage (10, 20 and 40 mg/kg body weight), intramuscular injection (5, 10 and 20 mg/kg body weight) and dipping (10, 20 and 40 ppm; 1 h). Samples were taken at 1, 3, 5, 10, 15, 24, 30, 48, 96, 144, 216, 264 and 360 h post-administration. In oral dosage of 10, 20 and 40 mg/kg, it's peak concentrations were $3.62{\pm}0.97$, $5.20{\pm}0.70$ and $11.18{\pm}0.87{\mu}g/ml$, respectively at 10 h post-administration, but AM was not measurable at 144, 360 and 360 h post-administration, respectively. In intramuscular injection of 5, 10 and 20 mg/kg, it's peak concentrations were $6.92{\pm}1.29{\mu}g/m1$, $9.89{\pm}2.22{\mu}g/ml$ and $19.85{\pm}2.97{\mu}g/ml$, respectively at 5 h post-administration, but AM was not measurable at 216, 264 and 264 h post-administration, respectively. In dipping of 10, 20 and 40 ppm, it's peak concentrations were $4.39{\pm}1.10$, $9.57{\pm}1.51$ and $11.61{\pm}1.92{\mu}g/ml$, respectively at 3 h post-administration, but AM was not measurable at 264, 264 and 360 h post-administration, respectively. Therefore, the plasma distribution and elimination levels of AM in olive flounder were dosage-dependant manner in all administration routes.
암피실린 (ampicillin, AM) 을 넙치 (평균체중 300g, $20{\pm}1.0^{\circ}C$) 에 1일 1회 경구 (10, 20, 40mg/kg), 근육주사(5, 10, 20mg/kg), 약욕 (10, 20, 40ppm; 1시간) 한 다음, 경시적 (1시간~360시간) 으로 혈장 내 AM의 잔류량을 HPLC로써 분석하였다. 10, 20 및 40mg/kg로 경구 투여한 경우, 최대혈중농도가 모두 10시간째 각각 $3.62{\pm}0.97$, $5.20{\pm}0.70$ 및 $11.18{\pm}0.87{\mu}g/ml$로 나타났으며, 이들은 각각 144, 360 및 360시간째 검출되지 않았다. 5, 10 및 20mg/kg의 농도로 근육주사한 경우, 최대 혈중농도가 모두 5시간째 $6.92{\pm}1.29$, $9.89{\pm}2.22$ 및 $19.85{\pm}2.97{\mu}g/ml$를 나타내었으며, 이들은 각각 216, 264 및 264시간째 혈중에서 검출되지 않았다. 10, 20 및 40ppm의 농도로 약욕한 경우, 최대혈중농도가 모두 3시간째 $4.39{\pm}1.10$, $9.57{\pm}1.51$ 및 $11.61{\pm}1.92{\mu}g/ml$로 나타났으며, 이들은 각각 264, 264 및 360시간째 혈중에서 검출되지 않았다. 이로써 경구, 주사 및 약욕 투약에 의한 넙치 혈액 중 분포와 배설정도는 투약량에 의존적 경향이라는 것을 알 수 있었다.
