Food Science of Animal Resources (한국축산식품학회지)

Korean Society for Food Science of Animal Resources (한국축산식품학회)
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Rheological Properties of Dough and Quality Characteristics of Bread Containing Whey Ferment Cultured by L. acidophilus KCCM 32820 and P. freudenreichii KCCM 31227

L. acidophilus KCCM 32820과 P. freudenreichii KCCM 31227로 배양한 유청발효물을 첨가한 반죽 레올로지 및 식빵의 품질특성

  • 이정훈 (건국대학교 분자생명공학과) ;
  • 최미정 (건국대학교 분자생명공학과) ;
  • 정구춘 (건국대학교 화학과) ;
  • 이시경 (건국대학교 분자생명공학과)
  • Received : 2012.07.05
  • Accepted : 2012.11.06
  • Published : 2012.12.31
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Abstract

This study was carried out to evaluate the effects of whey ferment containing L. acidophilus KCCM 32820 and P. freudenreichii KCCM 31227 on the quality characteristics of white pan bread. Instrumental analysis such as alveograph, gelatinization temperature, texture analysis, retrogradation rate was determined. In an alveograph test, $P_{max}$ value in the treatment was higher than that in the control, but extensibility of dough in the control showed to be higher than in the treatment, so test dough showed more strength than the control. In terms of DSC analysis for gelatinization, temperature there were no significant differences of $T_p$ and ${\Delta}H$ between the control and the treatment. In hardness analysis by rheometer, dough containing whey ferment revealed lower values than the control. From the analysis of the organic acid contents, propionic acid was not detected in the control, however 1.13 mg/g of propionic acid was detected in the treatment. In the retrogradation analysis by DSC, the test delayed slightly compared to the control.

본 실험은 유청을 L. acidophilus KCCM 32820과 P. freudenreichii KCCM 31227로 2단계 발효하여 만든 유청 발효물을 식빵 반죽에 첨가하여 반죽의 레올로지 및 품질특성에 미치는 영향을 분석하여 제품의 품질을 개선하고자 하였다. 레올로지 분석 중 Alveograph에서 $P_{max}$는 시험구가 높았고, 신장성은 대조구가 높아 시험구의 반죽 강도가 강한 것으로 나타났다. DSC에 의한 호화특성은 피크온도($T_p$)와 엔탈피(H)에서 대조구와 시험구에서 차이가 없어 유청발효물이 영향을 주지 않는 것으로 나타났다. 제품의 수분함량은 시험구가 다소 높았으나 유의적 차이가 없었다. 조직감은 저장 3일째까지 시험구가 부드러웠으나 4일 이후에는 두 시료간에 차이가 없었다. 유기산 분석 결과 대조구에서는 프로피온산이 검출되지 않았으나 시험구에서는 1.13 mg/g이 검출되었고 젖산은 대조구보다 시험구에서 높게 검출되었다. 노화특성은 대조구에 비하여 엔탈피 ${\Delta}H$(J/g)가 낮은 시험구에서 느린 것으로 나타났다.

Keywords

References

  1. Alummoottil, N. J., Korappatti, S., Moothandasseri, S. S., Revammab, R., and Subramoney N. M. (2005) Gelatinisation properties of Cassava starch in the presence of salts, acids and oxidising agents. Starch 57, 547-555. https://doi.org/10.1002/star.200500412
  2. American Association of Cereal Chemists. (1985) Approved methods of AACC. Methods 10-10b, 54-30.
  3. Ara, K. (2003) Science of propionic acid bacteria. New Food Indust. 45, 58-63.
  4. Bettge, A., Rubenthaler, G. L., and Pomeranz, Y. (1989) Alveograph algorithms to predict functional properties of wheat in bread and cookie baking. Cereal Chem. 66, 81-86.
  5. Cha, U. J. (2003) A study on properties of the flour-ferment with Lactobacillus acidophilus and the quality of noodles using the ferment. Ph. D. thesis, Konkuk Univ., Seoul, Korea.
  6. Cogan, T. M. (1995) Flavour production by dairy starter cultures. J. Appl. Microbiol., Symposium Supplement. 79, 49S-64S. https://doi.org/10.1111/j.1365-2672.1995.tb03123.x
  7. Corsetti, A., Gobbetti, M., De Marco, B., Balestrieri, F., Russi, L., and Rossi, J. (2000) Combined effect of sour dough lactic acid bacteria and additives on bread firmness and staling. J. Agri. Food Chem. 48, 3044-3051. https://doi.org/10.1021/jf990853e
  8. Daeschel, M. A. (1989) Antimicrobial substances from lactic acid bacteria for use as food preservatives. Food Technol. 43, 164-166.
  9. Datta, R. (1981) Acidogenic fermentation of corn stover. Biotechnol. Bioeng. 23, 61-77. https://doi.org/10.1002/bit.260230106
  10. Erdogdu-Arnoczky, N., Czuchajowska, Z., and Pomeranz, Y. (1996) Functionality of whey and casein in fermentation and in breadmaking by fixed and optimized procedures. Cereal Chem. 73, 309-316.
  11. Gallardo-Escamilla, F. J., Kelly, A. L., and Delahunty, C. M. (2005) Sensory characteristics and related volatile flavor compound profiles of different types of whey. Dairy Sci. 88, 2689-2699. https://doi.org/10.3168/jds.S0022-0302(05)72947-7
  12. Gobbetti, M., Simonetti, M. S., Rossi, J., Cossignani, L., Corsetti, A., and Damiani, P. (1994) Free D- and L-amino acid evolution during sour dough fermentation and baking. J. Food Sci. 59, 881-884. https://doi.org/10.1111/j.1365-2621.1994.tb08149.x
  13. Hettinga, D. H. and Reinbold, G. W. (1972) The propionic acid bacteria-A review II. Metabolism. J. Milk Food Technol. 35, 358-372.
  14. Hujanen, M. and Linko, Y. Y. (1996) Effect of temperature and various nitrogen sources on L-lactic acid production by Lactobacillus casei. Appl. Microbiol. Biotechnol. 45, 307-313. https://doi.org/10.1007/s002530050688
  15. Indrani, D., Prabhasankar, P., PJyotsna, R. G., and Venkateswara, R. (2007) Influence of whey protein concentrate on the rheological characteristics of dough, microstructure and quality of unleavened flat bread (parotta). Food Res. Int. 40, 1254-1260. https://doi.org/10.1016/j.foodres.2007.08.005
  16. Kamila, G., Jacek, N., and Zbigniew, C. (2008) Comparison of the growth of Lactobacillus acidophilus and Bifidobacterium bifidum species in media supplemented with selected saccharides including probiotics. Acta Sci. Pol., Technol. Aliment. 7, 5-20.
  17. Kenichi, H., Nobuo, Y., and Naoki, T. (2000) Bifidogenic growth stimulator produced by propionic acid bacteria. Milk Sci. 49, 161-167.
  18. Kim, S. G., Cho, N. J., and Kim, Y. H. (1999) Science of bread and cake. B & C world Co., Ltd., Seoul, p. 219.
  19. Korean Food Code. (2002) Korean Food & Drug Administration. pp. 3-4.
  20. Lee, J. H., Yun, M. S., Bog, G. H., An, H. G., Woo, H. S., and Lee, J. J. (2010) New Principle of Bread and Cake. Gigumoonwhasa Co., Seoul, pp. 56, 110.
  21. Lee, M. G. (2003) Quality characteristics of frozen dough bread prepared with flour ferments containing wheat flour Koji and lactic acid bacteria. Ph. D. dissertation, Konkuk Univ., Seoul, Korea.
  22. Maleki, M., Hoseney, R. C., and Matterm, P. J. (1980) Effect of loaf volume, moisture content, and protein quality on the softness and staling rate of bread. Cereal Chem. 57, 138-140.
  23. Owen, R. F. (1985) Food chemistry. Marcel Dekker, Inc., New York and Basel, USA. pp. 664-665.
  24. Paul, S. and Diana, S. (1987) Dictionary of microbiology and molecular biology, 2nd ed. A Wiley Interscience Publication Co. Ltd., USA. p. 991.
  25. Russell, P. L. and Oliver, G. (1989) The effect of pH and NaCl content on starch gel aging. A study by differential scanning calorimetry and rheology. J. Cereal Sci. 10, 123-125. https://doi.org/10.1016/S0733-5210(89)80041-4
  26. SAS (2007) User's guide. SAS Institute: Cary, NC, USA.
  27. Song, J. C. and Park, H. J. (1998) New food processing and storage. Hyoilmunwhasa Co., Seoul, p. 136.
  28. Vaughan, L. C. (1986) Metabolism of aspartate by Propionibacterium freudenreichii subsp. shermanii: Effect on lactate fermentation. Appl. Environ. Microbiol. 52, 359-365.
  29. Zeleznak, K. J. and Hoseney, R. C. (1986) The role of water in the retrogradation of wheat starch gels and bread crumb. Cereal Chem. 63, 407-411.