Ocean and Polar Research

Korea Institute of Ocean Science & Technology (한국해양과학기술원)
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Inhibitory Effect of Zostera japonica on Growth of Human Cancer Cells

애기거머리말 추출물의 암세포 성장 억제효과

  • Jung, Myung Eun (Division of Marine Environment & Bioscience, College of Maritime Sciences, Korea Maritime University) ;
  • Hong, Joo Wan (Division of Marine Environment & Bioscience, College of Maritime Sciences, Korea Maritime University) ;
  • Lee, Jung Im (Division of Marine Environment & Bioscience, College of Maritime Sciences, Korea Maritime University) ;
  • Kong, Chang-Suk (Department of Food and Nutrition, College of Medical and Life Sciences, Silla University) ;
  • Chang, Jae-Soo (Department of Environmental Engineering, College of Engineering, Korea Maritime University) ;
  • Seo, Youngwan (Division of Marine Environment & Bioscience, College of Maritime Sciences, Korea Maritime University)
  • 정명은 (한국해양대학교 해양과학기술대학 해양환경.생명과학부) ;
  • 홍주완 (한국해양대학교 해양과학기술대학 해양환경.생명과학부) ;
  • 이정임 (한국해양대학교 해양과학기술대학 해양환경.생명과학부) ;
  • 공창숙 (신라대학교 의생명과학대학 식품영양학과) ;
  • 장재수 (한국해양대학교 공과대학 환경공학과) ;
  • 서영완 (한국해양대학교 해양과학기술대학 해양환경.생명과학부)
  • Received : 2012.05.29
  • Accepted : 2012.11.12
  • Published : 2012.12.30
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Abstract

In this study, crude extracts of the marine eelgrass Zostera japonica and their solvent-partitioned fractions were evaluated for their inhibitory effect against AGS, HT-1080 and MCF-7 human cancer cells using MTT assay. Each of the crude extracts (acetone/methylene, chloride, and methanol) of Z. japonica showed a significant inhibitory effect on the growth of human cancer cells. The combined crude extracts were partitioned between $CH_2Cl_2$ and water. The organic layer was further partitioned between 85% aq. MeOH and n-hexane, and the aqueous layer was then fractionated into n-BuOH and $H_2O$, successively. Growth inhibition effects of solvent-partitioned fractions from Z. japonica on human cancer cells increased in a dose-dependent manner. Among these tested samples, the 85% aq. MeOH fraction revealed good inhibitory effects on the growth of AGS and HT-1080 human cancer cells, while the n-hexane fraction exhibited good inhibitory effects on the growth of AGS and MCF-7 human cancer cells. In addition, 85% aq. MeOH and n-hexane fractions enhanced mRNA expression of p53 gene. These results suggest that there is further scope for the isolation of active compounds from Z. japonica, which should show much stronger anticancer activity.

Keywords

References

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