DOI QR코드

DOI QR Code

효모세포벽추출물에 의한 고추내 salicylic acid 생성유도

Induction of Salicylic Acid Production in Pepper by Yeast Cell Wall Extract

  • 강대선 (한경대학교 바이오.정보기술대학원) ;
  • 조수묵 (국립농업과학원 기능성식품과) ;
  • 강희완 (한경대학교 바이오.정보기술대학원)
  • Kang, Dae Sun (Graduate School of Biotechnology and Information Technology, Hankyong National University) ;
  • Cho, Soo Muk (Functional Functional Food & Nutrition Division, Department of Agrofood Resources) ;
  • Kang, Hee-Wan (Graduate School of Biotechnology and Information Technology, Hankyong National University)
  • 투고 : 2012.10.30
  • 심사 : 2012.11.13
  • 발행 : 2012.12.31

초록

효모추출물 4 mg/mL를 고추유묘에 경엽 및 뿌리 관주처리 24시간 후에 salicylic acid(SA)함량을 ultra high performance liquid chromatography(UHPLC)분석방법으로 측정한 결과 yeast cell wall extract(YCWE)처리구에서 대조구의 SA처리구와 동일한 peak가 검출되어 SA가 생성 유도 되는 것으로 확인 되었다. SA는 YCWE를 경엽처리 48시간 후 20.29 ${\mu}g/g$으로 가장 높게 나타났으며 관주처리보다 약 3.7배의 높은 SA검출함량을 보였으나 처리 후 72시간부터는 점차적으로 SA함량이 감소하는 양상을 보였다.

Yeast cell wall extract (YCWE) was treated on leaves and roots of pepper seedlings at the dosage of 4 mg/mL and salicylic acid (SA) production in pepper was detected by ultra high performance liquid chromatography (UHPLC). The SA production in pepper stem was induced by YCWE. SA was produced at the highest level of 20.29 ${\mu}g/g$ after 48 hrs of foliar spray with YCWE, which is 3.7 times higher than that of root perfusion with YCWM. SA production was gradually reduced after 72 hrs of YCWE treatment.

키워드

참고문헌

  1. Bent, A. F. and Mackey, D. 2007. Elicitors, effectors, and R genes: the new paradigm and a lifetime supply of questions. Annu. Rev Phytopathol. 45:399-436. https://doi.org/10.1146/annurev.phyto.45.062806.094427
  2. Dempsey, D. A., Shah, J. and Klessig, D. F. 1999. Salicylic acid and disease resistance in plants. Crit. Rev. Plant Sci. 18:547-575. https://doi.org/10.1080/07352689991309397
  3. Durrant, W. E. and Dong, X. 2004. Systemic acquired resistance. Annu. Rev. Phytopathol. 42:185-209. https://doi.org/10.1146/annurev.phyto.42.040803.140421
  4. Edgar, C. I., McGrath, K. C., Dombrecht, B., Manners, J. M., Maclean, D. C., Schenk, P. M. and Kazan, K. 2006. Salicylic acid mediates resistance to the vascular wilt pathogen Fusarium oxysporum in the model host Arabidopsis thaliana. Aust. Plant Pathol. 35:581-591. https://doi.org/10.1071/AP06060
  5. Huff, G. R., Huff, W. E., Farnell, M. B., Rath, N. C., Solis de Los Santos, F. and Donoghue, A. M. 2010. Bacterial clearance, heterophil function, and hematological parameters of transportstressed turkey poults supplemented with dietary yeast extract. Poult. Sci. 89:447-56. https://doi.org/10.3382/ps.2009-00328
  6. Kitagawa, T., Shirai, T., Takasaki, S. and Kinoshita, M. 2005. Induction of resistance to crown and root rot and powdery mildew on tomato by yeast extract. Engei Gakkai Zasshi Bessatsu. 74:413.
  7. Minami, T., Kitagawa, T. and Kinoshita, M. 2005. Induction of resistance to Bacterial soft rot, Bacterial rot and Bacterial spot on lettuce by yeast extract. Engei Gakkai Zasshi Bessatsu 74:189. https://doi.org/10.2503/jjshs.74.189
  8. Minami, T., Tanaka, T., Takasaki, S. and Kawamura, K. 2011. In vivo bioluminescence monitoring of defense gene expression in response to treatment with yeast cell wall extract. Plant. Biotech. 28:481-484. https://doi.org/10.5511/plantbiotechnology.11.1020a
  9. Nurnberger, T., Brunner, F., Kemmerling, B. and Piater, L. 2004. Innate immunity in plants and animals: striking similarities and obvious differences. Immunol. Rev. 198:249- 266. https://doi.org/10.1111/j.0105-2896.2004.0119.x
  10. Obara, N., Mitsuhara, I., Seo, S., Ohashi, Y., Hasegawa, M. and Matsuura, Y. 2007. Mechanism of PR gene expression by treatment of tobacco leaves with yeast extract(AGREVO EX). Jpn. J. Phytopathol. 73:94-101. https://doi.org/10.3186/jjphytopath.73.94
  11. Raacke, I. C., Rad, U. von., Mueller, M. J. and Berger, S. 2006. Yeast increases resistance in Arabidopsis against Pseudomonas syringae and Botrytis cinerea by Salicylic Acid-dependent as well as-independent Mechanisms. Mol. Plant-Microbe Interact.19:1138-1146. https://doi.org/10.1094/MPMI-19-1138
  12. Suzuki, H., Reddy, M. S. S., Naoumkina, M., Aziz, N., May, G. C., Huhman, D. V., Summer, L. W., Blount, J. W., Mendes, P. and Dixon, R. A. 2005. Methyl jasmonate and yeast elicitor induce differential transcriptional and metabolic re-programming in cell suspension cultures of the model legume. Medicago truncatula. Planta. 220:696-707.
  13. Spletzer, M. E. and Enyedi, A. J. 1999. Salicylic acid induces resistance to Alternaria solaniin hydroponically grown tomato. Phytopathol. 89:722-727. https://doi.org/10.1094/PHYTO.1999.89.9.722
  14. Verberne, M. C., Brouwer, N., Delbianco, F., Linthorst, H. J. M., Bol, J. F. and Verpoorte, R. 2002. Method for the extraction of the volatile compound salicylic acid from tobacco leaf material. Phytochem. Anal. 13:45-50. https://doi.org/10.1002/pca.615
  15. Verhagen, B. W. M., Glazebrook, J., Zhu, T., Chang, H. S., van Loon, L. C. and Pieterse, C. M. J. 2004. The transcriptome of rhizobacteria induced systemic resistance in Arabidopsis. Mol. Plant-Microbe Interact. 17:895-908. https://doi.org/10.1094/MPMI.2004.17.8.895
  16. Yang S. S., Kim C. H., Cho E. K. and Lee E. J. 1991. Distribution and characteristics of suppressive soil to Phytophthora bligh to fred-pepper in Korea. Res. Rep. RDA. 33:18-22. (in Korean).
  17. Zimmerli, L., Stein, M., Lipka, V., Schulze-Lefert, P. and Somerville, S. 2004. Host and non-host pathogens elicit different jasmonate/ethyleneresponses in Arabidopsis. Plant J. 40:633-646. https://doi.org/10.1111/j.1365-313X.2004.02236.x