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Induction and Proliferation of Adventitious Roots in Codonopsis spp.

더덕 속 식물의 부정근 유도와 증식

  • Kim, Ji Ah (Division of Forest Biotechnology, Korea Forest Research Institute) ;
  • Park, Eung Jun (Division of Forest Biotechnology, Korea Forest Research Institute) ;
  • Choi, Yong Eui (Department of Forest Resources, Kangwon National University)
  • 김지아 (국립산림과학원 산림생명공학과) ;
  • 박응준 (국립산림과학원 산림생명공학과) ;
  • 최용의 (강원대학교 산림자원학과)
  • Received : 2012.10.04
  • Accepted : 2012.12.04
  • Published : 2012.12.30

Abstract

We have established adventitious root culture systems of Codonopsis lanceolata, Codonopsis pilosula and Codonopsis ussuriensis. Root segments of C. lanceolata were the best explants for induction of adventitious roots and the number of adventitious root for explant was highest on solid medium with $0.5mg/{\ell}$ NAA and produced $18.8{\pm}1.9$ roots per explant. Root segments of C. pilosula were the best explants for induction of adventitious roots and the number of adventitious root for explant was highest on solid medium with $1.0mg/{\ell}$ NAA and produced $8.5{\pm}1.8$ roots per explant. Leaf segments of C. ussuriensis were the best explants for induction of adventitious roots and the number of adventitious root for explant was highest on solid medium with $0.5mg/{\ell}$ NAA and produced $7.8{\pm}0.4$ roots per explant. In liquid culture, the best production of adventitious root (fresh weight) was obtained in 1/2 MS medium with $1.0mg/{\ell}$ NAA. This study demonstrated for the first time to produce adventitious roots in C. pilosula and C. ussuriensis.

Keywords

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