Asian Pacific Journal of Cancer Prevention

  • 제13권7호
  • /
  • Pages.3507-3511
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  • 2012
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  • 1513-7368(pISSN)
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  • 2476-762X(eISSN)
아시아태평양암예방학회 (Asian Pacific Journal of Cancer Prevention)
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Silencing of Lysyl Oxidase Gene Expression by RNA Interference Suppresses Metastasis of Breast Cancer

  • Liu, Jian-Lun (Department of Breast Surgery, Cancer Hospital, Guangxi Medical University) ;
  • Wei, Wei (Department of Breast Surgery, Cancer Hospital, Guangxi Medical University) ;
  • Tang, Wei (Department of Breast Surgery, Cancer Hospital, Guangxi Medical University) ;
  • Jiang, Yi (Department of Breast Surgery, Cancer Hospital, Guangxi Medical University) ;
  • Yang, Hua-Wei (Department of Breast Surgery, Cancer Hospital, Guangxi Medical University) ;
  • Li, Jing-Tao (Department of Breast Surgery, Cancer Hospital, Guangxi Medical University) ;
  • Zhou, Xiao (Department of Breast Surgery, Cancer Hospital, Guangxi Medical University)
  • 발행 : 2012.07.31
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초록

Objective: The aim of this study was to investigate possible mechanisms of LOX gene effects on invasion and metastasis of breast cancer cells by RNA interference. Methods: LOX-RNAi-LV was designed, synthesized, and then transfected into a breast cancer cell line (MDA-MB-231). Expression of LOX, MMP-2 and MMP-9 was determined by real-time PCR, and protein expression of LOX by Western blotting. Cell migration and invasiveness were assessed with Transwell chambers. A total of 111 cases of breast cancer tissues, cancer-adjacent normal breast tissues, and 20 cases of benign lesion tissues were assessed by immunohistochemistry. Results: Expression of LOX mRNA and protein was suppressed, and the expression of MMP-2 and MMP-9 was significantly lower in the RNAi group than the control group (P<0.05), after LOX-RNAi-LV was transfection into MDA-MB-231 cells. Migration and invasion abilities were obviously inhibited. The expression of LOX protein in breast cancer, cancer-adjacent normal breast tissues and benign breast tumor were 48.6% (54/111), 26.1% (29/111), 20.0% (4/20), respectively, associations being noted with clinical stage, lymph node metastasis, tumor size and ER, PR, HER2, but not age. LOX protein was positively correlated with MMP-2 and MMP-9. Conclusion: LOX displayed an important role in invasion and metastasis of breast cancer by regulating MMP-2 and MMP-9 expression which probably exerted synergistic effects on the extracellular matrix (ECM).

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참고문헌

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