Pediatric Infection and Vaccine

The Korean Society of Pediatric Infectious Diseases (대한소아감염학회)
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Immunoassay of Pertussis According to Ages

백일해에 대한 연령별 면역혈청학적 연구

  • Lee, Soo Young (Department of Pediatrics, The Catholic University of Korea) ;
  • Choi, Ui Yoon (Department of Pediatrics, The Catholic University of Korea) ;
  • Kim, Ju Sang (Department of Internal Medicine, The Catholic University of Korea) ;
  • Ahn, Joong Hyun (Department of Internal Medicine, The Catholic University of Korea) ;
  • Choi, Jung Hyun (Department of Internal Medicine, The Catholic University of Korea) ;
  • Ma, Sang Hyuk (Department of Pediatrics, Changwon Fatima Hospital) ;
  • Park, Joon Soo (Department of Pediatrics, Soon Cheon Hyang University) ;
  • Kim, Hwang Min (Department of Pediatrics, Wonju Yonsei University) ;
  • Kang, Jin Han (Department of Pediatrics, The Catholic University of Korea)
  • 이수영 (가톨릭대학교 의과대학 소아과학교실) ;
  • 최의윤 (가톨릭대학교 의과대학 소아과학교실) ;
  • 김주상 (가톨릭대학교 의과대학 내과학교실) ;
  • 안중현 (가톨릭대학교 의과대학 내과학교실) ;
  • 최정현 (가톨릭대학교 의과대학 내과학교실) ;
  • 마상혁 (창원파티마병원 소아청소년과) ;
  • 박준수 (순천향대학교 의과대학 소아과학교실) ;
  • 김황민 (연세대학교 원주의과대학 소아과학교실) ;
  • 강진한 (가톨릭대학교 의과대학 소아과학교실)
  • Received : 2012.01.08
  • Accepted : 2012.02.16
  • Published : 2012.08.25
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Abstract

Purpose: We conducted the immunoassay of pertussis according to ages, in order to evaluate protective immunity against pertussis in Korean populations. Methods: Healthy subjects were enrolled at four university hospitals in Korea. The subjects were grouped as seven age groups (every 10 years). Antibodies against pertussis toxin (PT) in sera were measured by enzyme linked immunosorbent assay (ELISA) kits. Geometric mean concentrations (GMC) of antibodies and the ratios of the subjects with seroprotective antibody levels were determined. The subjects with antibody titers ${\geq}24.0EU/mL$ were considered to seroprotective as the manufacturer's protocol. Results: Total 1,605 subjects (age: 2 months-65 years) participated in this study, and their GMC was $56.16{\pm}50.54EU/mL$. Among seven age groups, age group <11 year showed the highest GMC ($64.78{\pm}53.24EU/mL$) (P<0.001). In the analysis of the ratios of the subjects with seroprotective antibody titers, 68.2% of the subjects were proven to seroprotective, and age group <11 year also showed the highest ratio (76.5%) (P<0.001). Conclusions: We found that adolescences or adults (age group ${\geq}11$ year) showed lower levels of antibody against pertussis and lower ratio of the subjects with seroprotective antibody titers than children (age group <11 year).

목적: 우리나라의 백일해에 대한 연령별 방어면역 상태를 평가하고자 면역혈청학적 연구를 시행하였다. 방법: 국내 4개 대학병원에 방문한 건강한 소아, 청소년 및 성인을 대상으로 하였다. 대상을 연령에 따라 7개 연령군으로 구분하였고 ELISA 방법을 이용하여 백일해의 항체가를 측정하였다. 연령군별 기하 평균항체가와 각 연령군별 양성 항체가를 보이는 피험자의 비율을 조사하였다. 제조사의 지침대로, 항체가가 24.0 EU/mL 이상인 경우를 양성으로 판정하였다. 결과: 전체 1,605명의 피험자가 연구에 참여하였고, 기하 평균항체가는 $56.16{\pm}50.54EU/mL$였다. 연령군별로는 11세 미만 소아 연령군($64.78{\pm}53.24EU/mL$)이 가장 높은 기하 평균항체가를 보였다(P<0.001). 양성 항체가를 보인 피험자의 비율은 전체 대상의 68.2%였고, 11세 미만 소아 연령군(76.5%)이 가장 높은 비율을 보였다(P<0.001). 결론: 소아 연령군과 비교하여, 청소년과 성인 연령군에서 백일해에 대한 낮은 기하 평균항체가를 나타냈으며 양성 항체가를 갖는 피험자의 비율이 낮다는 것을 확인하였다.

Keywords

References

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