Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
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Characterization of Mouse Interferon-Induced Transmembrane Protein-1 Expression in Mouse Testis

  • Lee, Ran (Department of Animal & Food Bioscience, College of Natural Science, Konkuk University) ;
  • Park, Hyun Jung (Department of Animal & Food Bioscience, College of Natural Science, Konkuk University) ;
  • Lee, Won Young (Department of Animal & Food Bioscience, College of Natural Science, Konkuk University) ;
  • Kim, Ji Hyuk (National Institute of Animal Science, RDA) ;
  • Kim, In Chul (National Institute of Animal Science, RDA) ;
  • Kim, Dong Woon (National Institute of Animal Science, RDA) ;
  • Lee, Sung Dae (National Institute of Animal Science, RDA) ;
  • Jung, Hyun Jung (National Institute of Animal Science, RDA) ;
  • Kim, Jong Moon (Department of Rehabilitation, School of Medicine, Konkuk University) ;
  • Yoon, Hyung Moon (Department of Orthopaedic Surgery, School of Medicine, Konkuk University) ;
  • Kwon, Hyuk Jung (Department of Family Medicine, School of Medicine, Konkuk University) ;
  • Song, Hyuk (Department of Animal & Food Bioscience, College of Natural Science, Konkuk University)
  • Received : 2012.09.14
  • Accepted : 2012.09.25
  • Published : 2012.09.30
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Abstract

Interferon induced transmembrane protein-1 (Ifitm-1) has been reported to have an important role in primordial germ cell formation, and it has expressed in female reproductive organ. In the present study, Ifitm-1 gene expression was identified in testes and all part of epididymis using western immunoblot and immunohistochemistry. Interestingly, Ifitm-1 expression was observed on the head of spermatozoa. To investigate the role of Ifitm-1 gene expression in behavior of spermatozoa after acrosome reaction, fresh sperm was incubated with calcium ionophore to induce acrosome reaction, whereas the expression of Ifitm-1 was not altered after the acrosome reaction. Then to identify the effect of Ifitm-1 in sperm motility and other seminal parameters, different concentration of Ifitm-1 antibody was incubated with spermatozoa, and seminal parameters were assessed using computer-assisted semen analysis (CASA). Interestingly, motility, progressive, and VAP were increased in the sperm with Ifitm-1 antibody treated compared to rabbit serum, however other parameters such as straightness were not changed. In order to identify the functional significance of Ifitm-1 in fertilization, capacitated spermatozoa were pre-incubated with anti-Ifitm-1 antibody and subsequently examined the ability to adhere to mouse oocytes. However, any defection or alteration in sperm-egg fusion was not found, Ifitm-1 antibody treated or non-treated spermatozoa showed a normal penetration. Although the precise role of Ifitm-1 in sperm motility and following fertilization need to be elucidated, this study suggests that the activation of Ifitm-1 on the sperm may enhance the motility of spermatozoa in mice.

Keywords

References

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