Nutrition Research and Practice

The Korean Nutrition Society (한국영양학회)
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Carbohydrase inhibition and anti-cancerous and free radical scavenging properties along with DNA and protein protection ability of methanolic root extracts of Rumex crispus

  • Shiwani, Supriya (Department of Medical Biotechnology, College of Biomedical Sciences, Kangwon National University) ;
  • Singh, Naresh Kumar (Department of Animal Biotechnology, College of Animal Life Sciences, Kangwon National University) ;
  • Wang, Myeong Hyeon (Department of Medical Biotechnology, College of Biomedical Sciences, Kangwon National University)
  • Received : 2012.05.01
  • Accepted : 2012.08.08
  • Published : 2012.10.31
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Abstract

The study elucidated carbohydrase inhibition, anti-cancerous, free radical scavenging properties and also investigated the DNA and protein protection abilities of methanolic root extract of Rumex crispus (RERC). For this purpose, pulverized roots of Rumex crispus was extracted in methanol (80% and absolute conc.) for 3 hrs for $60^{\circ}C$ and filtered and evaporated with vacuum rotary evaporator. RERC showed high phenolic content ($211{\mu}g$/GAE equivalent) and strong 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ($IC_{50}$ = 42.86 (absolute methanol) and $36.91{\mu}g/mL$ (80% methanolic extract)) and reduced power ability. Furthermore, RERC exhibited significant protective ability in $H_2O_2/Fe^{3+}$/ascorbic acid-induced protein or DNA damage and percentage inhibition of the HT-29 cell growth rate following 80% methanolic RERC exposure at $400{\mu}g/mL$ was observed to be highest ($10.2%{\pm}1.03$). Moreover, methanolic RERC inhibited ${\alpha}$-glucosidase and amylase effectively and significantly (P < 0.05). Conclusively, RERC could be considered as potent carbohydrase inhibitor, anti-cancerous and anti-oxidant.

Keywords

References

  1. Hazra B, Sarkar R, Mandal S, Biswas S, Mandal N. Studies on antioxidant and antiradical activities of Dolichos biflorus seed extract. Afr J Biotechnol 2009;8:3927-33.
  2. Espin JC, Soler-Rivas C, Wichers HJ. Characterization of the total free radical scavenger capacity of vegetable oils and oil fractions using 2,2-diphenyl-1-picrylhydrazyl radical. J Agric Food Chem 2000;48:648-56. https://doi.org/10.1021/jf9908188
  3. Slavnic Z. Polygonales-Polygonaceae. In: Josifovic M, editor. Flora of SR Serbia, Vol. III. Belgrade: Serbian Academy of Sciences and Arts; 1972. p.68-86.
  4. Yildirim A, Mavi A, Kara AA. Determination of antioxidant and antimicrobial activities of Rumex crispus L. extracts. J Agric Food Chem 2001;49:4083-9. https://doi.org/10.1021/jf0103572
  5. Tyler VE. The Honest Herbal: A Sensible Guide to the Use of Herbs and Related Remedies, 3rd ed. New York: Pharmaceutical Products Press, An imprint of the Haworth Press, Inc.; 1993. p.325-6.
  6. Burrill LC. Curly Dock and Broadleaf Dock, Rumex Crispus L. and Rumex Obtusifolius L. Oregon State University Extension Service, Washington State University Extension Service, University of Idaho Extension Service; 1992. Available from: http://ir.library.oregonstate.edu/xmlui/bitstream/handle/1957/16791/pnw398.pdf.
  7. Rutledge CR, McLendon T. An Assessment of Exotic Plant Species of Rocky Mountain National Park: Summary Information for Remaining Exotic Plant Species. Colorado: Department of Rangeland Ecosystem Science, Colorado State University; 2002. p.97. United States Geological Survey, Northern Prairie Wildlife Research Center Home Page; Available from: http://www.npwrc.usgs.gov/resource/plants/explant/index.htm.
  8. Hu W, Shen W, Wang MH. Free radical scavenging activity and protective ability of methanolic extract from Duchesnea indica against protein oxidation and DNA damage. J Food Sci Nutr 2009;14:277-82. https://doi.org/10.3746/jfn.2009.14.4.277
  9. Hu W, Heo SI, Wang MH. Antioxidant and anti-inflammatory activity of Kalopanax pictus leaf. J Korean Soc Appl Biol Chem 2009;52:360-6. https://doi.org/10.3839/jksabc.2009.064
  10. Ghasemi K, Ghasemi Y, Ebrahimzadeh MA. Antioxidant activity, phenol and flavonoid contents of 13 citrus species peels and tissues. Pak J Pharm Sci 2009;22:277-81.
  11. Que F, Mao L, Zhu C, Xie G. Antioxidant properties of Chinese yellow wine, its concentrate and volatiles. Lebenson Wiss Technol 2006;39:111-7. https://doi.org/10.1016/j.lwt.2005.01.001
  12. Singh N, Rajini PS. Free radical scavenging activity of an aqueous extract of potato peel. Food Chem 2004;85:611-6. https://doi.org/10.1016/j.foodchem.2003.07.003
  13. McDougall GJ, Shpiro F, Dobson P, Smith P, Blake A, Stewart D. Different polyphenolic components of soft fruits inhibit ${\alpha}$-amylase and ${\alpha}$-glucosidase. J Agric Food Chem 2005;53:2760-6. https://doi.org/10.1021/jf0489926
  14. Ali H, Houghton PJ, Soumyanath A. ${\alpha}$-Amylase inhibitory activity of some Malaysian plants used to treat diabetes; with particular reference to Phyllanthus amarus. J Ethnopharmacol 2006;107:449-55. https://doi.org/10.1016/j.jep.2006.04.004
  15. Kizil G, Kizil M, Ceken B. Protective ability of ethanol extracts of Hypericum scabroides Robson & Poulter and Hypericum triquetrifolium Turra against protein oxidation and DNA damage. Food Sci Biotechnol 2009;18:130-6.
  16. Qian ZJ, Jung WK, Byun HG, Kim SK. Protective effect of an antioxidative peptide purified from gastrointestinal digests of oyster, Crassostrea gigas against free radical induced DNA damage. Bioresour Technol 2008;99:3365-71. https://doi.org/10.1016/j.biortech.2007.08.018
  17. Frankel EN. Natural and biological antioxidants in food and biological systems, their mechanism of action, applications and implications. Lipid Technol 1995;7:77-80.
  18. Kizil G, Kizil M, Ceken B, Yavuz M, Demir H. Protective ability of ethanol extracts of Hypericum scabrum L. and Hypericum retusum aucher against the protein oxidation and DNA damage. Int J Food Prop 2011;14:926-40. https://doi.org/10.1080/10942910903491181
  19. Loft S, Fischer-Nielsen A, Jeding IB, Vistisen K, Poulsen HE. 8-Hydroxydeoxyguanosine as a urinary biomarker of oxidative DNA damage. J Toxicol Environ Health 1993;40:391-404. https://doi.org/10.1080/15287399309531806
  20. Je JY, Ahn CB, Oh MJ, Kang SY. Antioxidant activity of a red seaweed Polysiphonia morrowii extract. Food Sci Biotechnol 2009;18:124-9.
  21. Yamagishi S, Nakamura K, Takeuchi M. Inhibition of postprandial hyperglycemia by acarbose is a promising therapeutic strategy for the treatment of patients with the metabolic syndrome. Med Hypotheses 2005;65:152-4. https://doi.org/10.1016/j.mehy.2004.12.008
  22. Ferrari CK, Torres EA. Biochemical pharmacology of functional foods and prevention of chronic diseases of aging. Biomed Pharmacother 2003;57:251-60. https://doi.org/10.1016/S0753-3322(03)00032-5
  23. Wang J, Yuan X, Jin Z, Tian Y, Song H. Free radical and reactive oxygen species scavenging activities of peanut skins extract. Food Chem 2007;104:242-50. https://doi.org/10.1016/j.foodchem.2006.11.035
  24. Je JY, Kim SK. Reactive oxygen species scavenging activity of aminoderivatized chitosan with different degree of deacetylation. Bioorg Med Chem 2006;14:5989-94. https://doi.org/10.1016/j.bmc.2006.05.016
  25. Ayed-Boussema I, Bouaziz C, Rjiba K, Valenti K, Laporte F, Bacha H, Hassen W. The mycotoxin Zearalenone induces apoptosis in human hepatocytes (HepG2) via p53-dependent mitochondrial signaling pathway. Toxicol In Vitro 2008;22:1671-80. https://doi.org/10.1016/j.tiv.2008.06.016
  26. Chiu SM, Xue LY, Usuda J, Azizuddin K, Oleinick NL. Bax is essential for mitochondrion-mediated apoptosis but not for cell death caused by photodynamic therapy. Br J Cancer 2003;89:1590-7. https://doi.org/10.1038/sj.bjc.6601298

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