Characterization and Distribution of Various Flavobacterium sp. in Lake Soyang

소양호에 존재하는 새로운 Flavobacterium의 분포와 특징

  • Kim, Haneul (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies) ;
  • Joung, Yochan (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies) ;
  • Kang, Heeyoung (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies) ;
  • Lee, Beom-Il (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies) ;
  • Jang, Tae Yong (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies) ;
  • Joh, Kiseong (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies)
  • 김하늘 (한국외국어대학교 생명공학과) ;
  • 정요찬 (한국외국어대학교 생명공학과) ;
  • 강희영 (한국외국어대학교 생명공학과) ;
  • 이범일 (한국외국어대학교 생명공학과) ;
  • 장태용 (한국외국어대학교 생명공학과) ;
  • 조기성 (한국외국어대학교 생명공학과)
  • Received : 2012.07.09
  • Accepted : 2012.08.23
  • Published : 2012.09.30

Abstract

In this study, samples were collected from Lake Soyang in Kangwon-do in order to isolate novel Flavobacterium sp. strains. Totally, 21 strains of Flavobacterium showing 97%~98% similarity in 16S rRNA were selected and thoroughly investigated individual characteristics and ecological differences. As results, we could categorize Flavobacterium isolated from Lake Soyang into four major node groups, where most of Flavobacterium belonged to single group. Next, fatty acid analyses were performed demonstrating similar pattern of the majority of fatty acids as either iso $C_{15:0}$ or summed feature 3 (comprised $C_{16:1}$ ${\omega}7c$ and/or $C_{16:1}$ ${\omega}6c$) of the other Flavobacterium. However, other phenotypic data were different from the other Flavobacterium sp. Group. Our data showed that genetically related species of Flavobacterium have been distributed in Lake Soyang. Those Flavobacterium strains were phenotypically different from previously reported genus of Flavobacterium species. Taken together, we speculated that isolated Flavobacterium strains from Lake Soyang might be ecologically important members to maintain ecosystem.

우리나라 최대 담수호인 소양호에 존재하는 미생물 중 Flavobacterium을 분리하여 그 특징을 조사하였다. 그 중에서 97%와 98% 사이의 상동성을 지니는 Flavobacterium을 대상으로 21종을 선택하였으며, 그 균주들의 생리학적, 생태학적 위치를 살펴보았다. 그 결과, 소양호에 존재하는 21종의 분리된 Flavobacterium은 4개의 주된 node에 위치하였으며, 그 중에서 대부분이 하나의 group에 속하여 있었다. 각 Flavoacterium 21종의 생리적인 특징은 기존의 보고된 Flavoacterium들과는 서로 다른 특징을 지니지만, total fatty acid의 분석결과 iso $C_{15:0}$과 summed feature 3 (comprised $C_{16:1}$ ${\omega}7c$ and/or $C_{16:1}$ ${\omega}6c$)가 주요 성분으로 전형적인 Flavobacterium의 특징을 지닌 것으로 나타났다. 따라서 소양호내에는 유전적 유연관계가 있는 아변종의 Flavobacterium이 존재하며, 기존에 알려진 종들과는 다른 형질을 가지고 있는 것으로 보아 소양호 생태계의 생물 종 다양성이 유지되고 있는 것으로 판단된다.

Keywords

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