Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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A Novel Helicosporium Isolate and Its Antimicrobial and Cytotoxic Pigment

  • Choi, Hye Jung (Interdisplinary Program for Biotechnology and Department of Biology, Changwon National University) ;
  • Lee, Sang Myeong (SM Biovision Co., Ltd., Business Incubation Center, Gyeongsang National University) ;
  • Kim, Sun-Hee (Department of Biochemistry, Pusan National University School of Medicine) ;
  • Kim, Dong Wan (Department of Microbiology, Changwon National University) ;
  • Choi, Young Whan (Department of Horticultural Bioscience, Pusan National University) ;
  • Joo, Woo Hong (Interdisplinary Program for Biotechnology and Department of Biology, Changwon National University)
  • Received : 2012.04.26
  • Accepted : 2012.05.02
  • Published : 2012.09.28
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Abstract

One Helicosporium strain, isolated from a wilted chestnut tree, evidenced in vitro antimicrobial activity against various types of bacteria and fungi, and generated a diffusible pigment. The antimicrobial compounds and the diffusible pigment of the Helicosporium sp. isolate were purified via solvent fractionation, column chromatography, and recycling preparative chromatography. Both the major antimicrobial compound and the diffusible pigment were identified as 2-methylresorcinol via nuclear magnetic resonance spectroscopy. Therefore, 2-methylresorcinol, a diffusible pigment generated by Helicosporium sp., appears to be an active antimicrobial principle. This pigment also exhibited considerable cytotoxicity against mammalian cells.

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References

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