Journal of Microbiology and Biotechnology

  • 제22권8호
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  • Pages.1107-1112
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  • 2012
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Detection and Identification of Vibrio Species Using Whole-Cell Protein Pattern Analysis

  • Lee, Chae-Yoon (Department of Food Science & Technology, Ewha Woman's University) ;
  • Hong, Yeun (Institute of Life Sciences & Resources and Department of Food Science & Biotechnology, Kyung Hee University) ;
  • Ryu, Jio (Institute of Life Sciences & Resources and Department of Food Science & Biotechnology, Kyung Hee University) ;
  • Kim, Young-Rok (Institute of Life Sciences & Resources and Department of Food Science & Biotechnology, Kyung Hee University) ;
  • Oh, Sang-Suk (Department of Food Science & Technology, Ewha Woman's University) ;
  • Lee, Soon-Ho (Food Microbiology Division, Korea Food & Drug Administration) ;
  • Hwang, In-Gyun (Food Microbiology Division, Korea Food & Drug Administration) ;
  • Kim, Hae-Yeong (Institute of Life Sciences & Resources and Department of Food Science & Biotechnology, Kyung Hee University)
  • 투고 : 2012.01.02
  • 심사 : 2012.03.28
  • 발행 : 2012.08.28
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초록

Outbreaks of foodborne diseases associated with Vibrio species such as V. parahaemolyticus, V. vulnificus, and V. cholerae frequently occur in countries having a dietary habit of raw seafood consumption. For rapid identification of different Vibrio species involved in foodborne diseases, whole-cell protein pattern analysis for 13 type strains of 12 Vibrio species was performed using SDS-PAGE analysis. Pathogenic Vibrio species such as V. parahaemolyticus, V. vulnificus, V. cholerae, V. alginolyticus, V. fluvialis, and V. mimicus were included in the 12 Vibrio species used in this study. Each of the 12 Vibrio species showed clearly specific band patterns of its own. Two different strains of V. parahaemolyticus showed two different SDS-PAGE whole-cell protein patterns, giving the possibility of categorizing isolated strains in the same V. parahaemolyticus species into two subgroups. The 36 Vibrio isolates collected from sushi restaurants in Busan were all identified as V. parahaemolyticus by comparing their protein patterns with those of Vibrio type strains. The identified isolates were categorized into two different subgroups of V. parahaemolyticus. The whole-cell protein pattern analysis by SDS-PAGE can be used as a specific, rapid, and simple identification method for Vibrio spp. involved in foodborne diseases at the subspecies level.

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