Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Analysis of Expressed Sequence Tags from the Antarctic Psychrophilic Green Algae, Pyramimonas gelidicola

  • Jung, Woongsic (Division of Polar Life Sciences, Korea Polar Research Institute) ;
  • Lee, Sung Gu (Division of Polar Life Sciences, Korea Polar Research Institute) ;
  • Kang, Se Won (Department of Parasitology, College of Medicine and Frontier Inje Research for Science and Technology, Inje University) ;
  • Lee, Yong Seok (Department of Parasitology, College of Medicine and Frontier Inje Research for Science and Technology, Inje University) ;
  • Lee, Jun Hyuck (Division of Polar Life Sciences, Korea Polar Research Institute) ;
  • Kang, Sung-Ho (Department of Polar Sciences, University of Science and Technology) ;
  • Jin, Eon Seon (Department of Life Science, College of Natural Sciences, Hanyang University) ;
  • Kim, Hak Jun (Division of Polar Life Sciences, Korea Polar Research Institute)
  • Received : 2012.01.03
  • Accepted : 2012.03.04
  • Published : 2012.07.28
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Abstract

Expressed sequence tags (ESTs) from the Antarctic green algae Pyramimonas gelidicola were analyzed to obtain molecular information on cold acclimation of psychrophilic microorganisms. A total of 2,112 EST clones were sequenced, generating 222 contigs and 219 singletons, and 200 contigs and 391 singletons from control ($4^{\circ}C$) and cold-shock conditions ($-2^{\circ}C$), respectively. The complete EST sequences were deposited to the DDBJ EST database (http://www.ddbj.nig.ac.jp/index-e.html) and the nucleotide sequences reported in this study are available in the DDBJ/EMBL/GenBank. These EST databases of Antarctic green algae can be used in a wide range of studies on psychrophilic genes expressed by polar microorganisms.

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