Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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The Magas1 Gene is Involved in Pathogenesis by Affecting Penetration in Metarhizium acridum

  • Cao, Yueqing (Genetic Engineering Research Center, College of Bioengineering, Chongqing University, Chongqing Engineering Research Center for Fungal Insecticides and Key Laboratory of Functional Gene and Regulation Technologies under Chongqing Municipal Education Commission) ;
  • Zhu, Xiangxian (Genetic Engineering Research Center, College of Bioengineering, Chongqing University, Chongqing Engineering Research Center for Fungal Insecticides and Key Laboratory of Functional Gene and Regulation Technologies under Chongqing Municipal Education Commission) ;
  • Jiao, Run (Genetic Engineering Research Center, College of Bioengineering, Chongqing University, Chongqing Engineering Research Center for Fungal Insecticides and Key Laboratory of Functional Gene and Regulation Technologies under Chongqing Municipal Education Commission) ;
  • Xia, Yuxian (Genetic Engineering Research Center, College of Bioengineering, Chongqing University, Chongqing Engineering Research Center for Fungal Insecticides and Key Laboratory of Functional Gene and Regulation Technologies under Chongqing Municipal Education Commission)
  • Received : 2011.11.22
  • Accepted : 2012.02.22
  • Published : 2012.07.28
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Abstract

Appressorium is a specialized infection structure of filamentous pathogenic fungi and plays an important role in establishing a pathogenic relationship with the host. The Egh16/Egh16H family members are involved in appressorium formation and pathogenesis in pathogenic filamentous fungi. In this study, a homolog of Egh16H, Magas1, was identified from an entomopathogenic fungus, Metarhizium acridum. The Magas1 protein shared a number of conserved motifs with other Egh16/Egh16H family members and specifically expressed during the appressorium development period. Magas1-EGFP fusion expression showed that Magas1 protein was not localized inside the cell. Deletion of the Magas1 gene had no impact on vegetative growth, conidiation and appressorium formation, but resulted in a decreased mortality of host insect when topically inoculated. However, the mortality was not significant between the Magas1 deletion mutant and wild-type treatment when the cuticle was bypassed by injecting conidia directly into the hemocoel. Our results suggested that Magas1 may influence virulence by affecting the penetration of the insects' cuticle.

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References

  1. Ahren, D., M. Tholander, C. Fekete, B. Rajashekar, E. Friman, T. Johansson, and A. Tunlid. 2005. Comparison of gene expression in trap cells and vegetative hyphae of the nematophagous fungus Monacrosporium haptotylum. Microbiology 151: 789-803. https://doi.org/10.1099/mic.0.27485-0
  2. Cao, Y., M. Li, and Y Xia. 2011. Mapmi gene contributes to growth, stress tolerance and virulence of the entomopathogenic fungus Metarhizium acridum. J. Invertebr. Pathol. 108: 7-12. https://doi.org/10.1016/j.jip.2011.06.002
  3. Chou, K. C. and H. B. Shen. 2010. A new method for predicting the subcellular localization of eukaryotic proteins with both single and multiple sites: Euk-mPLoc 2.0. PLoS One 5: e9931. https://doi.org/10.1371/journal.pone.0009931
  4. Clarkson, J. M. and A. K. Charnley. 1996. New insights into the mechanisms of fungal pathogenesis in insects. Trends Microbiol. 4: 197-203. https://doi.org/10.1016/0966-842X(96)10022-6
  5. Gao, Q., K. Jin, S. H. Ying, Y. Zhang, G. Xiao, Y. Shang, et al. 2011. Genome sequencing and comparative transcriptomics of the model entomopathogenic fungi Metarhizium anisopliae and M. acridum. PLoS Genet. 7: e1001264. https://doi.org/10.1371/journal.pgen.1001264
  6. Grell, M. N., P. Mouritzen, and H. Giese. 2003. A Blumeria graminis gene family encoding proteins with a C-terminal variable region with homologues in pathogenic fungi. Gene 311: 181-192.
  7. He, M. and Y. Xia. 2009. Construction and analysis of a normalized cDNA library from Metarhizium anisopliae var. acridum germinating and differentiating on Locusta migratoria wings. FEMS Microbiol. Lett. 291: 127-135. https://doi.org/10.1111/j.1574-6968.2008.01447.x
  8. Justesen, A., S. Somerville, and H. Giese. 1996. Isolation and characterization of two novel genes expressed in germinating conidia of the obligate biotroph Erysiphe graminis f. sp. hordei. Gene 17: 131-135.
  9. Liu, J., Y. Cao, and Y. Xia. 2010. Mmc, a gene involved in microcycle conidiation of the entomopathogenic fungus Metarhizium anisopliae. J. Invertebr. Pathol. 105: 132-138. https://doi.org/10.1016/j.jip.2010.05.012
  10. Lomer, C. J., R. P. Bateman, D. L. Johnson, J. Langewald, and M. B. Thomas. 2001. Biological control of locusts and grasshoppers. Annu. Rev. Entomol. 46: 667-702. https://doi.org/10.1146/annurev.ento.46.1.667
  11. Peng, G., Z. Wang, Y. Yin, D. Zeng, and Y. Xia. 2008. Field trials of Metarhizium anisopliae var. acridum (Ascomycota: Hypocreales) against oriental migratory locusts, Locusta migratoria manilensis (Meyen) in Northern China. Crop Prot. 27: 1244-1250. https://doi.org/10.1016/j.cropro.2008.03.007
  12. Tang, Q. Y. and M. G. Feng. 2002. DPS Data Processing System for Practical Statistics. Science Press, Beijing.
  13. Vega, F. E., F. Posada, M. C. Aime, M. Pava-Ripoll, F. Infante, and S. A. Rehner. 2008. Entomopathogenic fungal endophytes. Biol. Control 46: 72-82. https://doi.org/10.1016/j.biocontrol.2008.01.008
  14. Wang, C. and R. J. St. Leger. 2007. The Metarhizium anisopliae perilipin homolog MPL1 regulates lipid metabolism, appressorial turgor pressure, and virulence. J. Biol. Chem. 282: 21110-21115. https://doi.org/10.1074/jbc.M609592200
  15. Xue, C., G. Park, W. Choi, L. Zheng, R. A. Dean, and J. R. Xu. 2002. Two novel fungal virulence genes specifically expressed in appressoria of the rice blast fungus. Plant Cell 14: 2107-2119. https://doi.org/10.1105/tpc.003426

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