Korean Journal of Clinical Laboratory Science (대한임상검사과학회지)
- Volume 44 Issue 1
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- Pages.24-30
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- 2012
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- 1738-3544(pISSN)
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- 2288-1662(eISSN)
Effects of Storage Buffer and Temperature on the Integrity of Human DNA
- Kim, Yun-Tae (Department of Genome research, Seoul Clinical Laboratories) ;
- Choi, Eun-Hee (Department of Genome research, Seoul Clinical Laboratories) ;
- Son, Bo-Kyoung (Department of Genome research, Seoul Clinical Laboratories) ;
- Seo, Eun-Hee (Department of Genome research, Seoul Clinical Laboratories) ;
- Lee, Eun-Kyoung (Department of Genome research, Seoul Clinical Laboratories) ;
- Ryu, Je-Kwon (Department of Genome research, Seoul Clinical Laboratories) ;
- Ha, Gi-Won (Department of Genome research, Seoul Clinical Laboratories) ;
- Kim, Jin-Seon (Department of Genome research, Seoul Clinical Laboratories) ;
- Kwon, Mi-Ran (Department of Genome research, Seoul Clinical Laboratories) ;
- Nam, Jae-Hoon (Department of Genome research, Seoul Clinical Laboratories) ;
- Kim, Young-Jin (Department of Genome research, Seoul Clinical Laboratories) ;
- Lee, Kyoung-Ryul (Department of Genome research, Seoul Clinical Laboratories)
- Received : 2012.03.05
- Accepted : 2012.03.29
- Published : 2012.03.30
Abstract
In this study, we have examined the effects of the storage time and temperature on DNA quality and have also studied the effects of the hydration buffer in which DNA is dissolved. This study was performed using 160 human blood samples collected with informed consent from 2007 to 2008 in the hospital where this cohort study was performed. The DNA extracted was dissolved using distilled water (DW) or Tris-EDTA (TE) buffer, and stored in the deep freezer or refrigerator for up to 10 weeks at