Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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Rapid Determination of Volatile Organic Compounds in Human Whole Blood Using Static Headspace Sampling with Gas Chromatography and Mass Spectrometry

  • Lee, Ji-Young (Biomolecules Function Research Center, Korea Institute of Science and Technology) ;
  • Kim, Seungki (Biomolecules Function Research Center, Korea Institute of Science and Technology) ;
  • Lee, Jong-Tae (Department of Environmental Health, Korea University) ;
  • Choi, Jong-Ho (Department of Chemistry, Korea University) ;
  • Lee, Jeongae (Biomolecules Function Research Center, Korea Institute of Science and Technology) ;
  • Pyo, Heesoo (Biomolecules Function Research Center, Korea Institute of Science and Technology)
  • Received : 2011.10.15
  • Accepted : 2012.09.04
  • Published : 2012.12.20
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Abstract

Headspace (HS) and headspace solid-phase microextraction (HS-SPME) were studied for extracting volatile organic compounds (VOCs) from whole blood, with chemical and instrumental variables being optimized for maximum sensitivity: incubation at $60^{\circ}C$, equilibration for 30 min, pH 11, and 2 mL injection volume. Both techniques provided accurate analyses, with detection limits of 0.05-0.1 ng $mL^{-1}$ and 0.05-0.5 ng $mL^{-1}$. HS showed better sensitivity, reproducibility, and analysis times than HS-SPME. Overall levels of chloroform in whole blood were found to be 0.05-5.84 ng $mL^{-1}$; detected levels of benzene were 0.05-2.20 ng $mL^{-1}$.

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