Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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The Effect of Black Garlic Extract on Lipid Metabolism in Restraint Stressed Rats

구속스트레스를 가한 흰쥐의 지질 대사에 대한 흑마늘 추출물의 효과

  • Received : 2012.09.28
  • Accepted : 2012.11.26
  • Published : 2012.11.30
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Abstract

In order to determine the effective concentration, 2, 7, and 14 brix black garlic extracts (BGE) were fed to restraint stressed rats and the lipid metabolism was analyzed. The dietary intake of the 14 brix BGE-fed group was $547.64{\pm}13.99$ g/4 weeks and it was significantly higher than that of the control group. The weight of all organs from the all BGE-fed groups showed no significant differences. While BGE concentration increased, BGE-fed groups tend to have low content of blood glucose. It was the lowest in the 14 brix BGE-fed group ($121.32{\pm}3.50$ mg/dl). Total cholesterol content was $54.54{\pm}1.63{\sim}65.84{\pm}2.61$ mg/dl. Total cholesterol content of the 14 brix BGE-fed group was the lowest and, in addition, lower than the normal group. Triglyceride content of serum showed the same tendency. HDL-cholesterol concentrations of the restraint stressed control (RSC) group were significantly higher than those of 7 and 14 brix BGE-fed groups. LDL and VLDL-cholesterol contents showed contrary results. GOT, GTP, and ALP activity of serum were lower when BGE concentration was higher. It is expected that BGE has a liver protective activity. In the liver, total lipids and triglyceride contents were significantly lower in BGE-fed groups than in the ISC group. However, total cholesterol content showed no significant difference between groups. TBARS content in liver of the 2 brix BGE-fed group was not significantly different than control, while 7 and 14 brix BGE-fed groups were lower than control. Glycogen content in the liver was only significantly lower in the 14 brix BGE-fed group than in the RSC group. Findings indicate that feed of BGE over 7 brix contributes to resolving the restraint-stressed rats by improving the lipid metabolism and liver protective activity.

흑마늘의 구속 스트레스에 대한 유효농도를 확인하고자 2, 7 및 14 brix로 조절한 흑마늘 추출물을 급이한 흰쥐의 체내 지질대사를 중심으로 분석하였다. 흑마늘 추출물 14 brix 급이군의 식이섭취량은 $547.64{\pm}13.99$ g/4 weeks로 대조군에 비해 유의적으로 높았다. 모든 장기에서 흑마늘 추출물의 첨가 급이에 따른 무게의 변화는 통계적인 유의차는 없었다. 흑마늘 추출물 첨가 급이군의 혈당은 흑마늘 추출물의 농도가 높아질수록 낮아지는 경향을 보여 2 brix 추출물 급이군의 혈당은 $142.97{\pm}6.51$ mg/dl 였으나 14 brix 급이군에서는 $121.32{\pm}3.50$ mg/dl로 정상군 보다도 더 낮았다. 총 콜레스테롤 함량은 $54.54{\pm}1.63{\sim}65.84{\pm}2.61$ mg/dl의 범위였는데, 14 brix의 흑마늘 추출물 첨가 급이군의 혈청 총 콜레스테롤 농도는 $54.54{\pm}1.63$ mg/dl로 가장 낮은 함량이었으며, 정상군에 비해서도 유의적으로 낮은 함량이었다. 중성지질의 함량도 동일한 경향이었다. HDL-콜레스테롤 농도는 구속대조군에 비해 흑마늘 추출물 7 및 14 brix 급이군에서 유의적으로 높았으며, LDL VLDL-콜레스테롤은 이와 상반되는 결과를 나타내었다. 혈청 GOT 및 GTP 활성도는 흑마늘 추출물의 농도가 높아질수록 낮아져 흑마늘 추출물의 급이는 간보호 활성을 나타낼 것으로 기대된다. 간장내 총 지질 및 중성지질의 함량은 구속대조군에 비해 흑마늘 추출물 급이군에서 유의적으로 낮았으나 총콜레스테롤은 서로간에 유의차가 없었다. 간장내 지질 과산화물인 TBARS 함량은 2 brix의 흑마늘 추출물 급이군은 구속대조군과 유의적인 차이가 없었으나 7 및 14 brix 급이군은 정상군보다 더 낮은 함량이었다. 간장내 글리코겐 함량은 구속대조군에 비해 14 brix 흑마늘 추출물 급이군에서만 유의적으로 낮았다. 이상의 결과들을 종합하여 볼 때 7 brix 이상의 흑마늘 추출물 급이는 흰쥐의 지질대사를 개선하고, 간보호 활성을 나타냄으로써 구속 스트레스로 해소에 기여할 것으로 추정된다.

Keywords

References

  1. Ames, B. N., Shigenaga, M. K. and Hagen, T. M. 1993. Oxidant, antioxidant and the degenerative disease of aging. Proc. Natl. Acad. Sci. USA 90, 7915-7922. https://doi.org/10.1073/pnas.90.17.7915
  2. Aronne, L., Nelinson, D. and Lillo, J. 2009. Obesity as a disease state: A new paradigm for diagnosis and treatment. Clin. Cornerstone 9, 9-29.
  3. Castelli, W. P., Garrison, R. J., Wilson, P. W. F., Abborr, R. D., Kalousdian, S. and Kannel, W. B. 1986. Incidence of coronary heart disease and lipoprotein cholesterol leavels. The Framingham Study JAMA 256, 2835-2845.
  4. Cheung, P. C. K. 1998. Plasma and hepatic cholesterol levels and fecal neutral sterol extcretion are alter in hamsters fed straw mushroom diets. J. Nutr. 128, 1512-1530.
  5. Choi, D. J., Lee, S. J., Kang, M. J., Cho, H. S. and Sung, N. K. 2008. Physicochemical characteristics of black garlic (Allium sativum L.). J. Korean Soc. Food Nutr. 37, 465-471. https://doi.org/10.3746/jkfn.2008.37.4.465
  6. Folch, J., Lees, M. and Stanley, G. H. 1957. A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226, 497-502.
  7. Friedewald, W. T., Levy, R. I. and Fredrickson, D. S. 1972. Estimation of the concentration of low density lipoprotein cholesterol in plasma, without use of the preparative ultracentrefuge. Clin. Chem. 18, 499-502.
  8. Frings, C. S., Fendley, T. W., Dunn, R. T. and Queen, C. A. 1972. Improved determination of total serum lipids by the sulfo-phospho-vanillin reaction. Clin. Chem. 18, 763-764.
  9. Haenen, G. R., Paquay, J. B., Korthouwer, R. E. and Best, A. 1997. Peroxynitrite scavenging by flavonoids. Biochem. Biophys Res. Commun. 236, 591-593. https://doi.org/10.1006/bbrc.1997.7016
  10. Haglund, O., Loustarinen, R., Wallin, R., Wibell, I. and Saldeen, T. 1991. The effect of oil on triglycerides, cholesterol, fibrinogen and malondi- aldehyde in mand supplemented with vitamin. Eur. J. Nutr. 121, 165-172.
  11. Hong, M. S. 1992. The culture of food in Korea. pp. 79. Kyomoonsa, Seoul.
  12. Kang, S. M., Shim, J. Y., Hwang, S. J., Hong, S. G., Jang, H. E. and Park, M. H. 2003. Effects of Saengshik supplementation on health improvement in diet-induced hypercholesterolemic rats. J. Korean Soc. Food Sci. Nutr. 32, 906-912. https://doi.org/10.3746/jkfn.2003.32.6.906
  13. Kim, H. K., Jo, K. S., Shin, D. E. and Kim, I. H. 1987. Effect of phosphate complex treatment on the quality of dried garlic flakes. Korean J. Food Sci. Tochnol. 19, 75-80.
  14. Knekt, P., Jarvinen, R., Reunanen, A. and Martela, J. 1997. Flavonoid intake and coronary mortality in Finland : a cohort study. Br. Med. J. 312, 478-481.
  15. Koo, K. B., Chung, J. M. and Lee, H. S. 1998. Biochemical evaluation of nutritional status of protein and lipid in patients with alcoholic liver disease. J. Korean Soc. Food Sci. Nutr. 27, 1236-1243.
  16. Lim, S. H., Jeong, H. Y., Won, H. Y., Kim, H. W., Choi, C. W., Jeong, H. S., Kim, Y. G. and Cho, S. I. 2012. Effects of Banhahubak-tang Extract on Psychological Stress. Kor. J. Herbology 27, 81-88. https://doi.org/10.6116/kjh.2012.27.4.81
  17. Medina-Campos, O. N., Barrera, D., Segoviano-Murillo, S. Rocha, D., Maldonado, P., Mendoza-Patin, N. and Pedraza-Chaverri, J. 2007. S-allylcysteine scavenges singlet oxygen and hypochlorous acid and protects LLC-PK1 cells of potassium dichromate-induced toxicity. Food Chem. Toxicol. 45, 2030-2039. https://doi.org/10.1016/j.fct.2007.05.002
  18. Orth Gom, K., Moser, V., Blom, M., Wamala, S. P. and Schenck Gustafesson, K. 1997. Survey of stress in women. Heart disease in Stockholm women is caused by both family- and-work-related stress. Lakartidningen. 632, 635-638.
  19. Pirnay, F., Scheen, A. J., Gautier, J. F., Lacroix, M. and Mosora, F. 1995. Exogenous glucose oxidation during exercise in relation to the power output. Int. J. Sports Med. 16, 456-460. https://doi.org/10.1055/s-2007-973037
  20. Raju, J. and Mehta, R. 2009. Cancer chemopreventive and therapeutic effects of diosgenin, a food saponin. Nutr. Cancer 61, 27-35. https://doi.org/10.1080/01635580802357352
  21. Rona, C. and Berardesca, E. 2008. Aging skin and food supplements: the myth and the truth. Clin. Dermatol. 26, 641-647. https://doi.org/10.1016/j.clindermatol.2007.09.002
  22. Sheo, H. J. 1999. Effect of garlic on the blood lipids and other serum components in rats. J. Korean Soc. Food Sci. Nutr. 28, 1339-1348.
  23. Shin, J. H., Choi, D. J., Chung, M. J., Kang, M. J. and Sung, N. K. 2008. Changes of physicochemical components and antioxidant activity of aged garlic at different temperatures. J. Korean Soc. Food Sci. Nutr. 37, 1174-1181. https://doi.org/10.3746/jkfn.2008.37.9.1174
  24. Shin, J. H., Choi, D. J., Lee, S. J., Cha, J. Y. and Sung, N. J. 2008. Antioxidant activity of black garlic (Allium sativum L.). J. Korean Soc. Food Sci. Nutr. 37, 965-971. https://doi.org/10.3746/jkfn.2008.37.8.965
  25. Shin, J. H., Jung, K. M., Lee, S. J., Yang, S. M., Rue, G. H. and Sung, N. J. 2009. Biological activities of dried garlic, red ginseng and their mixutre. J. Korean Soc. Food Sci. Nutr. 38, 1633-1639. https://doi.org/10.3746/jkfn.2009.38.12.1633
  26. Shin, J. H., Lee, H. G., Kang, M. J., Lee, S. J. and Sung, N. J. 2010. Antioxidant activity of solvent fraction from black garlic. J. Korean Soc. Food Sci. Nutr. 39, 933-940. https://doi.org/10.3746/jkfn.2010.39.7.933
  27. Shin, S. H. and Kim, M. K. 2004. Effect of dried powders or ethanol extracts of garlic flesh and peel on antioxidative capacity in 16-mouth-old rats. Korean J. Nutr. 37, 633-644.
  28. Uchiyama, M. and Mihara, M. 1978. Determination of malondialdehyde precursor in tissues by TBA test. Anal. Biochem. 86, 271-278. https://doi.org/10.1016/0003-2697(78)90342-1
  29. Wang, M. F., Shao, Y. J., Yi, G., Zhu, N. Q., Rngarajan, M., Lavoie, E. J. and Ho, C. T. 1998. Antioxidative phenolic compounds from sage (Salivia officinalis). J. Agric. Food Chem. 46, 4869-4873. https://doi.org/10.1021/jf980614b

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