생명과학회지 (Journal of Life Science)

  • 제22권11호
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  • Pages.1507-1514
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  • 2012
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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산수유 추출방법에 따른 항산화 기능 분석

Antioxidative Activity of Cornus officianalis Extracts Obtained by Four Different Extraction Techniques

  • 박은비 (동의대학교 임상병리학과) ;
  • 김혜선 (동의대학교 임상병리학과) ;
  • 신소연 (동의대학교 임상병리학과) ;
  • 지인해 (동의대학교 임상병리학과) ;
  • 김지현 (동의대학교 임상병리학과) ;
  • 김성구 ((주)바이오포트코리아) ;
  • 유병홍 ((주)바이오포트코리아) ;
  • 김병우 (동의대학교 블루바이오 소재개발 및 실용화 지원 센터) ;
  • 곽인석 (신라대학교 생물과학과) ;
  • 김문무 (동의대학교 화학과) ;
  • 정경태 (동의대학교 임상병리학과)
  • Park, Eun-Bi (Department of Clinical Laboratory Science, Dong-Eui University) ;
  • Kim, Hye-Sun (Department of Clinical Laboratory Science, Dong-Eui University) ;
  • Shin, So-Yun (Department of Clinical Laboratory Science, Dong-Eui University) ;
  • Ji, In-Ae (Department of Clinical Laboratory Science, Dong-Eui University) ;
  • Kim, Ji-Hyun (Department of Clinical Laboratory Science, Dong-Eui University) ;
  • Kim, Sung-Goo (Bioport Korea Ltd.) ;
  • Yoo, Byung Hong (Bioport Korea Ltd.) ;
  • Kim, Byung-Woo (Blue-Bio Industry RIC, Dong-Eui University) ;
  • Kwak, Inseok (Department of Biological Science, Silla University) ;
  • Kim, Moon-Moo (Department of Chemistry, Dong-Eui University) ;
  • Chung, Kyung Tae (Department of Clinical Laboratory Science, Dong-Eui University)
  • 투고 : 2012.09.18
  • 심사 : 2012.11.16
  • 발행 : 2012.11.30
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초록

산수유는 다양한 생리적 기능을 가지는 것으로 보고되어 있으며 또한 최근에 관심의 증가로 산수유로부터 유효성분의 추출 방법 개발은 산업적으로 매우 중요하며, 유효성분의 존재 여부를 분석하는 것은 산업적 상품의 진위와 품질을 판정하는데 중요한 수단이 된다. 본 연구는 loganin을 지표 물질로 하여 산수유로부터 유효성분을 추출하는 여러 방법을 비교 분석하였으며, 각 방법에 따라 추출한 시료의 기능성의 차이를 항산화 작용으로 분석하였다. 산수유로부터 항산화 성분을 추출하기 위하여 네 가지 방법을 적용하였다. 추출된 유효성분 지표물질로 loganin의 함량을 HPLC로 분석하였을 때 열수를 사용한 추출 방법이 알코올 추출 또는 효소 처리 방법 보다 효율적인 결과를 보여 주었다. 각 추출 시료의 항산화 기능은 DPPH radical 소거능, hydrogen peroxide scavenging assay, reducing power 측정으로 분석하였다. 추출 방법에 따라 항산화 기능이 차이가 있었으며, 열수 추출 시료가 DPPH radical 소거능과 hydrogen peroxide scavenging 효과가 우수하였고, 효소 처리 추출 시료는 reducing power가 우수하게 나타났다. 추출 방법에 따라 시료에 함유된 성분의 차이가 항산화 기능이 차이와 연관이 있다고 생각된다. 이 연구에서 얻은 결과는 산수유는 대조군으로 사용한 vitamin C에 준하는 항산화 성분을 함유하고 있으며, 이들 성분은 열수 추출 방법으로 효율적으로 추출되었으며, 열수 추출 방법은 산업적으로 응용될 수 있음을 제시하고 있다.

Oxidative stress leads to damage in all components of the cell, including proteins, lipids, and DNA due to imbalance between reactive oxygen species production and cellular detoxification. Phytochemicals are well-known to contain antioxidants, and their physiological role has been intensively studied. The fruit of Cornus officianalis has been used in oriental medicine and has been reported to have many functions. In this study, four different extraction techniques were applied to extract functional components from the fruit of Cornus officianalis, and the content of loganin, which is an antioxidant having DPPH radical and hydrogen peroxide scavenging activity and reducing power, was analyzed in each extract. Extraction techniques employed in this study were heat extraction by water, 70% ethanol extraction, enzyme treatment, and combination of enzyme treatment and heat extraction by water. All extracts contained 11.8-18.0 mg/g loganin and showed antioxidation function assayed by measuring DPPH radical and hydrogen peroxide scavenging activity and reducing power. Among them, heat extraction was the most effective technique, showing a maximum amount of loganin (18.0 mg/g) and antioxidative activity at 100 mg/ml concentration. Each extract showed very low cytotoxicity up to at 500 mg/ml but 10-20% cytotoxicity at 1,000 mg/ml by in vitro MTT assay.

키워드

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