Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Analysis of Genetic Diversity and Relationships of Korean Native Goat Populations by Microsatellite Markers

MS 표지를 이용한 한국재래염소 집단의 유전적 다양성 및 유연관계 분석

  • Suh, Sangwon (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Byun, Mijeong (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Kim, Young-Sin (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Kim, Myung-Jick (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Choi, Seong-Bok (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Ko, Yeoung-Gyu (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Kim, Dong-Hun (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Lim, Hyun-Tae (Department of Animal Science, Collage of Agriculture and Life Sciences, Gyeongsang National University) ;
  • Kim, Jae-Hwan (Animal Genetic Resources Station, National Institute of Animal Science, RDA)
  • 서상원 (농촌진흥청 국립축산과학원 가축유전자원시험장) ;
  • 변미정 (농촌진흥청 국립축산과학원 가축유전자원시험장) ;
  • 김영신 (농촌진흥청 국립축산과학원 가축유전자원시험장) ;
  • 김명직 (농촌진흥청 국립축산과학원 가축유전자원시험장) ;
  • 최성복 (농촌진흥청 국립축산과학원 가축유전자원시험장) ;
  • 고응규 (농촌진흥청 국립축산과학원 가축유전자원시험장) ;
  • 김동훈 (농촌진흥청 국립축산과학원 가축유전자원시험장) ;
  • 임현태 (경상대학교 농업생명과학대학 축산학과) ;
  • 김재환 (농촌진흥청 국립축산과학원 가축유전자원시험장)
  • Received : 2012.09.05
  • Accepted : 2012.11.02
  • Published : 2012.11.30
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Abstract

The level of genetic variation and relationships in three native Korean goat populations (Dangjin, Jangsu, and Tongyeong) as well as the populations of a farm were analyzed, based on 30 microsatellite markers. A total of 277 distinct alleles were observed across the four goat populations, and 102 (36.8%) of these alleles were unique to only one population. The mean observed heterozygosity and polymorphism information content were calculated as 0.461~0.651 and 0.462~0.679, respectively. In the NJ tree constructed based on Nei's $D_A$ genetic distance, the four populations represented four distinct groups. However, the genetic distances between each Korean native goat population and the farm population were two times those among the three native Korean breeds. The genetic structure within the three Korean native goat populations was also investigated. Cluster analysis, using the STRUCTURE software, suggested three clusters. The molecular information of genetic diversity and relationships in this study will be useful for the evaluation, conservation, and utilization of native Korean goat breeds as genetic resources.

본 연구는 30개의 MS 마커를 이용하여 한국재래염소 3개 집단(당진, 장수, 통영)과 1개 농가집단을 대상으로 집단 내 및 집단간의 유전적 다양성, 계통유전학적 유연관계 분석 및 한국재래염소 3개 집단간의 유전적 균일성을 검증하여 우리 고유유전자원으로서의 가치를 구명하고자 실시하였다. 대립유전자형 분석 결과, 총 277개의 대립유전자형 중 집단-특이 대립유전자형은 102개(36.8%)였으며, 다형성지수인 이형접합도의 관측치($H_O$)는 0.416~0.651, 다형정보량(PIC)은 0.462~0.679로 산출되었다. Nei의 $D_A$유전거리를 토대로 개체별 NJ 계통수를 작성한 결과 집단별로 독립적인 그룹을 형성하였는데, 한국재래염소 3개 집단 간의 유전거리에 비해 한국재래염소 집단과 농가 집단 간의 유전거리는 2배 이상을 보였다. 한국재래염소 집단의 실제적인 분류 및 분류된 군락의 균일도를 STRUCTURE software를 이용하여 분석한 결과, 실제 공시한 집단 수와 동일한 3개의 군락으로 분류가 가능했고, 각 집단에 대한 균일도는 통영(84.1%), 장수(78.1%), 당진(69.9%)의 결과를 나타냈다. 본 연구를 통하여 한국재래염소 집단의 유전적 다양성, 유연관계 및 유전적 균일성을 확인하였다. 본 연구에서 확인된 한국재래염소의 유전적 특성은 우리 고유자원에 대한 과학적인 근거자료이며, 나아가 가축유전자원에 대한 국가수준의 보존, 평가 및 이용에 활용될 수 있을 것으로 사료된다.

Keywords

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