Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Inhibition of Enzymatic Browning of Crown Daisy by Maillard Reaction Products

마이얄반응생성물(Maillard reaction product)에 의한 쑥갓의 효소적갈면 억제

  • Kim, Ji-Hae (Department of Food Science and Nutrition, Dong-A University) ;
  • Song, Hyeon-Seung (Department of Food Science and Nutrition, Dong-A University) ;
  • Park, Inshik (Department of Food Science and Nutrition, Dong-A University)
  • 김지혜 (동아대학교 식품영양학과) ;
  • 송현승 (동아대학교 식품영양학과) ;
  • 박인식 (동아대학교 식품영양학과)
  • Received : 2012.10.16
  • Accepted : 2012.11.08
  • Published : 2012.11.30
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Abstract

The study was conducted to investigate the effect of Maillard reaction products (MRPs) on enzymatic browning of crown daisy (Chrysanthmum coronarium var. spatiosum). The MRPs prepared by heating various amino acid and sugar at $90^{\circ}C$ caused a strong inhibitory effect on crown daisy polyphenol oxidase (PPO, ${\sigma}$-diphenol oxygen oxidoreductase, EC 1.10.3.1). As the reaction time of the solution containing glycine and glucose increased at $90^{\circ}C$, the production of MRPs was increased, whereas the amounts of glycine and glucose were decreased. Accordingly, the inhibitory effect of crown daisy PPO activity by MRPs was increased as the amounts of synthesized MRPs were increased. The MRPs synthesized from the various amino acids and sugars significantly reduced the PPO activity, particularly MRPs prepared by glutamine and xylose. The Michealis-Menten constant value ($K_m$) of crown daisy PPO with catechol as a substrate was 22.0 mM, and MRPs were a noncompetitive inhibitor against crown daisy PPO.

다양한 아미노산과 당을 $90^{\circ}C$에서 반응시켜 생성한 마이얄반응 생성물(Maillard reaction product)은 쑥갓에서 추출한 폴리페놀산화효소(polyphenol Oxidase, PPO)를 강하게 저해하였다. 글리신(glycine)과 포도당을 함유한 반응물으로부터 MRP의 생성은 $90^{\circ}C$에서 반응시간에 따라 직선적으로 증가하였으나 글리신과 포도당의 양은 상대적으로 감소하였다. 반응액에서 MRP의 증가함에 따라 반응액은 쑥갓 PPO활성을 비례적으로 강하게 저해하였다. 실험에 사용한 아미노산과 당중에서 글루타민과 자일로스를 아미노산과 당으로 이용했을 경우에 생성된 MRP가 쑥갓 PPO의 활성에 강한 저해제로 작용하였다. 카테콜을 기질로 사용한 쑥갓 PPO의 마이클레스 상수(Michaelis Menten constant)는 22.0 mM이었으며, MRP는 쑥갓 PPO의 비경쟁적 저해제로 작용하였다.

Keywords

References

  1. Ames, J. M. 1992. The Maillard reaction. pp. 99-153, In Hudson, B. (ed.), Biochemistry of Food Proteins. Elsevier Applied Science, London.
  2. Bedinghaus, A. J. and Ockerman, H. W. 1995. Antioxidative Maillard reaction products from reducing sugars and free amino acids in cooked ground pork patties. J. Food Sci. 60, 992-995. https://doi.org/10.1111/j.1365-2621.1995.tb06277.x
  3. Busch, J. M. 1999. Enzymatic browning in potatoes: a simple assay for polyphenol oxidase catalyzed reaction. Biochem. Educ. 27, 171-173. https://doi.org/10.1016/S0307-4412(99)00033-3
  4. Chandra, R., Bharagava, R. N. and Rai, V. 2008. Melanoidins as major colorant in sugarcane molasses based distillery effluent and its degradation. Bioresour. Technol. 99, 4648-4660. https://doi.org/10.1016/j.biortech.2007.09.057
  5. Ding, C., Chachin, K., Ueda, Y. and Wang, C. Y. 2002. Inhibition of loquat enzymatic browning by sulfhydryl compounds. Food Chem. 76, 213-218. https://doi.org/10.1016/S0308-8146(01)00270-9
  6. Friedman, M. 1996. Food Browning and Prevention: An Overview. The American Chemical Society, 44, 632-653.
  7. Gomyo, T., Kato, H., Udaka, K., Horikoshi, M. and Fujimaki, M. 1972. Chemical studies on melanoidins: Part III. Effects of heating on chemical properties of melanoidin prepared from glycine-xylose system. Agric. Biol. Chem. 36, 125-132. https://doi.org/10.1271/bbb1961.36.125
  8. Hayase, F., Hirashima, S., and Okamota, G. 1989. Kato H. Scavenging of active oxygen by mellanodines. Agric. Biol. Chem. 53, 3383-3385. https://doi.org/10.1271/bbb1961.53.3383
  9. Jang, M. S., Sanada, A., Ushio, H., Tanaka, M. and Ohshima, T. 2002. Inhibitory effects of 'Enokitake' mushroom extracts on polyphenol oxidase and prevention of apple browning. Lebens-Wissen. Technol. 35, 697-702. https://doi.org/10.1006/fstl.2002.0937
  10. Jiang, Y. and Fu, J. 1998. Inhibition of polyphenol oxidase and the browning control of litchi fruit by glutathione and citric acid. Food Chem. 62, 49-52. https://doi.org/10.1016/S0308-8146(97)00144-1
  11. Kawashima, K., Itoh, H. and Chibata, I. 1977. Antioxidant activity of browning products prepared from low molecular carbonyl compounds and amino acids. J. Agric. Food Chem. 25, 202-204. https://doi.org/10.1021/jf60209a036
  12. Langdon, T. T. 1987. Preventing browning in freshly prepared potatoes without the use of sulfiting agents. Food Technol. 41, 64-67.
  13. Lee, M., Kim, Y., Kim, N., Kim, G., Kim, S., Bang, K. and Park, I. 2002. Prevention of browning in potato with a heat-treated onion extract. Biosci. Biotechnol. Biochem. 66, 856-858. https://doi.org/10.1271/bbb.66.856
  14. Lee, M. and Park, I. 2005. Inhibition of potato polyphenol oxidase by Maillard reaction products. Food Chem. 91, 57-61. https://doi.org/10.1016/j.foodchem.2004.05.046
  15. Martinez, M. V. and Whitaker, J. R. 1995. The biochemistry and control of enzymatic browning. Trends Food Sci. Technol. 6, 195-200. https://doi.org/10.1016/S0924-2244(00)89054-8
  16. McEvily, A. J., lyengar, R. and Otwell, W. S. 1992. Inhibition of enzymatic browning in foods and beverages. Crit. Rev. Food Sci. Nutri. 32, 253-273. https://doi.org/10.1080/10408399209527599
  17. Morales, F. J., Somoza, V. and Fogliano, V. 2012. Physiological relevance of dietary melanoidins. Amino Acid 42, 1097-1109. https://doi.org/10.1007/s00726-010-0774-1
  18. Namiki, M. 1998. Chemistry of Maillard reactions: recent studies on the browning reaction mechanism and the development of antioxidants and mutagens. Adv. Food Res. 38, 115-183.
  19. Negishi, O., Negishi, Y. and Ozawa, T. 2002. Effects of food materials on removal of Allium-specific volatile sulfur compounds. J. Agric. Food Chem. 50, 3856-3861. https://doi.org/10.1021/jf020038q
  20. Negishi, O. and Ozawa, T. 2000. Inhibition of enzymatic browning and protection of sulfhydryl enzymes by thiol compounds. Phytochemistry 54, 481-487. https://doi.org/10.1016/S0031-9422(00)00125-4
  21. Nurten, H. 2005. The Maillard Reaction: Chemistry, Biochemistry and Implication, Royal Society of Chemistry, Cambridge, UK.
  22. Pizzocaro, F., Torreggiani, D. and Gilardi, G. 1993. Inhibition of apple polyphenol oxidase by ascorbic acid, citric acid and sodium chloride. J. Food Proc. Preser. 17, 21-30. https://doi.org/10.1111/j.1745-4549.1993.tb00223.x
  23. Plummer, D. T. 1985. An Introduction to Practical Biochemistry. McGraw Hill. London.
  24. Tan, B. K. and Harris, N. D. 1995. Maillard reaction products inhibit apple polyphenol oxidase. Food Chem. 53, 267-273. https://doi.org/10.1016/0308-8146(95)93932-H
  25. Wijewickkreme, A. N. and Kitts, D. D. 1977. Influence of reaction conditions on the oxidative behavior of model Maillard reaction products. J. Agric. Food Chem. 45, 4571- 4576.
  26. Zauberman, G., Ronen, R., Akerman, M. and Weksler, A., Rot, I. and Fuch, Y. 1991. Postharvest retention of the red color of litchi fruit pericarp. Scientia. Hort. 47, 89-97. https://doi.org/10.1016/0304-4238(91)90030-3