DOI QR코드

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Protective Effect of Ginsenoside R0 on Anoxic and Oxidative Damage In vitro

  • Jiang, Zhou (Key Laboratory of Chronobiology, Ministry of Health, Sichuan University) ;
  • Wang, Yuhui (Key Laboratory of Chronobiology, Ministry of Health, Sichuan University) ;
  • Zhang, Xiaoyun (Teaching Hospital of Chengdu University of Traditional Chinese Medicine) ;
  • Peng, Tao (Teaching Hospital of Chengdu University of Traditional Chinese Medicine) ;
  • Li, Yanqing (Teaching Hospital of Chengdu University of Traditional Chinese Medicine) ;
  • Zhang, Yi (Teaching Hospital of Chengdu University of Traditional Chinese Medicine)
  • 투고 : 2012.06.15
  • 심사 : 2012.10.22
  • 발행 : 2012.11.30

초록

To examine the neuroprotective effects of ginsenoside R0, we investigated the effects of ginsenoside R0 in PC12 cells under an anoxic or oxidative environment with Edaravone as a control. PC12 neuroendocrine cells were used as a model target. Anoxic damage or oxidative damage in PC12 cells were induced by adding sodium dithionite or hydrogen peroxide respectively in cultured medium. Survival ratios of different groups were detected by an AlamarBlue assay. At the same time, the apoptosis of PC12 cells were determined with flow cytometry. The putative neuroprotective effects of ginsenoside R0 is thought to be exerted through enhancing the activity of antioxidant enzymes Superoxide dismutases (SOD). The activity of SOD and the level of malondialdehyde (MDA) and intracellular reactive oxygen species (ROS), were measured to evaluate the protective and therapeutic effects of ginsenoside R0. Ginsenoside R0 treated cells had a higher SOD activity, lower MDA level and lower ROS, and their survival ratio was higher with a lower apoptosis rate. It is suggested that ginsenoside R0 has a protective effect in the cultured PC12 cells, and the protection efficiency is higher than Edaravone. The protective mechanisms of these two are different. The prevent ability of ginsenoside R0 is higher than its repair ability in neuroprotection in vitro.

키워드

참고문헌

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피인용 문헌

  1. Profiling and identification of the metabolites of ginsenoside Ro in rat faeces and urine after oral administration vol.242, pp.2, 2016, https://doi.org/10.1007/s00217-015-2531-x
  2. Effect of Korean Red Ginseng extraction conditions on antioxidant activity, extraction yield, and ginsenoside Rg1 and phenolic content: optimization using response surface methodology vol.40, pp.3, 2016, https://doi.org/10.1016/j.jgr.2015.08.001
  3. The beneficial effect of ginsenosides extracted by pulsed electric field against hydrogen peroxide-induced oxidative stress in HEK-293 cells vol.41, pp.2, 2017, https://doi.org/10.1016/j.jgr.2016.02.007
  4. Neuroprotective Effects of Ginsenosides against Cerebral Ischemia vol.24, pp.6, 2019, https://doi.org/10.3390/molecules24061102