The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Neuroprotective effect of Puerariae Radix extract on focal cerebral ischemia in mice

갈근 에탄올추출물의 국소뇌허혈 모델에 대한 뇌신경보호 효과

  • Song, Jungbin (Dept. of Herbal Pharmacology, College of Korean Medicine, Kyung Hee University) ;
  • Choi, Jin Gyu (Korea Institute of Science and Technology for Eastern Medicine (KISTEM), NeuMed Inc.) ;
  • Lee, Donghung (Korea Institute of Science and Technology for Eastern Medicine (KISTEM), NeuMed Inc.) ;
  • Gaire, Bhakta Prasad (Dept. of Herbal Pharmacology, College of Korean Medicine, Kyung Hee University) ;
  • Lee, Changwon (Dept. of Herbal Pharmacology, College of Korean Medicine, Kyung Hee University) ;
  • Bu, Youngmin (Dept. of Herbal Pharmacology, College of Korean Medicine, Kyung Hee University) ;
  • Choi, Ho-Young (Dept. of Herbal Pharmacology, College of Korean Medicine, Kyung Hee University) ;
  • Kim, Hocheol (Dept. of Herbal Pharmacology, College of Korean Medicine, Kyung Hee University)
  • 송정빈 (경희대학교 한의과대학 본초학교실) ;
  • 최진규 ((주)뉴메드 한의과학기술연구소) ;
  • 이동헌 ((주)뉴메드 한의과학기술연구소) ;
  • ;
  • 이창원 (경희대학교 한의과대학 본초학교실) ;
  • 부영민 (경희대학교 한의과대학 본초학교실) ;
  • 최호영 (경희대학교 한의과대학 본초학교실) ;
  • 김호철 (경희대학교 한의과대학 본초학교실)
  • Received : 2012.10.15
  • Accepted : 2012.11.09
  • Published : 2012.11.30
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Abstract

Objectives : The purpose of this study was to evaluate the neuroprotective effect of Pueraria lobata extract on focal cerebral ischemia in mice. Methods : Focal cerebral ischemia was induced by occlusion of the right middle cerebral artery using the intraluminal filament model. ICR male mice underwent 90 minutes of middle cerebral artery occlusion (MCAo) followed by 24 hours of reperfusion. Mice were administered Pueraria lobata extract orally at the dose of 300mg/kg just prior to reperfusion. Rotarod test and balance beam test were practiced to assess sensory-motor function 23 hours after MCAo. In rotarod test, the latency to fall on the accelerating rotarod was recorded for 5 min. In balance beam test, the score was graded according to number of slips and latency to cross. The infarct volume was measured 24 hours after MCAo using 2% 2,3,5-triphenyltetrazolium chloride (TTC) staining. Results : Pueraria lobata extract treated group showed significant reduction in infarct volume by 27.3% compared to control group (p<0.05). In rotatod test, it also showed significant extension of latency time compared to control group ($67.82{\pm}15.08$ vs. $5.62{\pm}1.06$, p<0.001). In contrast to performance in rotarod test, that in balance beam test did not improve with Pueraria lobata extract treatment. Conclusions : We conclude that Pueraria lobata extract has a significant neuroprotective effect and reduces damage of sensory-motor function in MCAo model. These findings suggest that Pueraria lobata could be a potent neuroprotective agent.

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References

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