Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Diagnosis of immunohistochemical marker expressed by a canine Sertoli cell tumor case

개 세르토리세포종 케이스에서 면역조직화학적 마커를 통한 진단

  • Kim, Sung-Jae (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) ;
  • Han, Jeong-Hee (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University)
  • 김성재 (강원대학교 수의과대학 수의학과 병리학실) ;
  • 한정희 (강원대학교 수의과대학 수의학과 병리학실)
  • Received : 2011.04.08
  • Accepted : 2011.09.14
  • Published : 2011.09.30
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Abstract

Sertoli cell tumor (SCT) of the testicle arises from the supporting cells within the seminiferous tubules. SCT is common in dogs, especially in cryptorchid testicles, but also has been reported in the stallion, ram, cat, and bull. Sertoli cell tumor sample was collected from 7-years male german shepherd. In this study, SCT arose from one testicle. Sample size is approximately 1.7 cm in diameter and it has a round form. In the microscopic, cells within the tumor variably resemble Sertoli cells (SCs) that normally populate the seminiferous tubules and interstitial area. There is abundant stroma of dense, mature fibrous connective tissue in SCT. In the immunohistochemical staining, cytokeratin AE1/AE3 was not expressed in the control and SCT. S-100 protein was expressed by SCs, germ cells and fibrous connective tissue of SCT. Melan A was expressed by leydig cells (LCs) of SCT. A study by using S-100 and melan A in canine SCT was almost never carried out. S-100 and melans A is considered to suggest for diagnosis and pathogenesis of canine SCTs. Inhibin-alpha and Vimentin were well known as the marekers of SCTs. Also, they were expressed by Sertoli cells and LSs of SCT in this study.

Keywords

References

  1. Angela LD, Julie AY, Susan JB, and Robert AF. 2006. Extratesticular interstitial and Sertoli cell tumors in previously neutered dogs and cats: A report of 17 cases. Can Vet J 47: 763-766.
  2. Arumugam T, Logsdon CD. 2010. S100P: a novel therapeutic target for cancer. Amino Acids: 1-7.
  3. Banco B, Giudice C, Veronesi MC, Gerosa E, Grieco V. 2010. An Immunohistochemical study of normal and neoplastic canine Sertoli cells. J Comp Path 143: 239-247. https://doi.org/10.1016/j.jcpa.2010.04.001
  4. Braun S, Pantel K, Muller P, Janni W, Hepp F, Kentenich CR, Gastroph S, Wischnik A, Dimpfl T, Kindermann G, Riethmuller G, Schlimok G. 2000. Cytokeratin-positive cells in the bone marrow and survival of patients with stage I, II, or III breast cancer. N Engl J Med 342: 525-533. https://doi.org/10.1056/NEJM200002243420801
  5. Busam KJ, Iversen K. Coplan KA, Old LJ, Stockert E, Chen YT, McGregor D, Jungbluth A. 1998. Immunoreactivity for A103, an antibody to melan-A (Mart-1), in adrenocortical and other steroid tumors. Am J Surg Pathol 22: 57-63. https://doi.org/10.1097/00000478-199801000-00007
  6. Chen YT, Stockert E, Jungbluth A, Tsang S, Coplan KA, Scanlan MJ, Old LJ. 1996. Serological analysis of Melan-A (MART-1), a melanocyte-specific protein homogeneously expressed in human melanomas. Proc Natl Acad Sci USA 93: 5915-5919. https://doi.org/10.1073/pnas.93.12.5915
  7. De Vico G, Papparella S, Di Guardo G. 1994. Number and size of silver-stained nucleoli (Ag-NOR clusters) in canine seminomas: correlation with histological features and tumour behaviour. J Comp Pathol 110: 267-273. https://doi.org/10.1016/S0021-9975(08)80279-4
  8. Dellman DH, Eurell JA. 2005. Male reproduct system. pp. 230-233. In: Dellman DH, Eurell JA(ed). Textbook of veterinary histology. 5th ed. LIPWW, United states.
  9. Iczkowski KA, Butler SL. 2006. New immunohistochemical markers in testicular tumors. Anal Quant Cytol Histol 28: 181-187.
  10. Kawakami E, Hirano T, Hori T, Tsutsui T. 2007. Testicular superoxide dismutase activity, heat shock protein 70 concentration and blood plasma inhibin-$\alpha$ concentration of dogs with a Sertoli cell tumor in a unilateral cryptorchid testis. J Vet Sci 69: 1259-1262. https://doi.org/10.1292/jvms.69.1259
  11. Lau SK, Weiss LM, Chu PG. 2007. D2-40 Immunohistochemistry in the differential diagnosis of seminoma and embryonal carcinoma: a comparative immunohistochemical Study with KIT (CD117) and CD30. Mod pathol 20: 320-325. https://doi.org/10.1038/modpathol.3800749
  12. Maymon BB, Paz G, Elliott DJ, Hammel I, Kleiman SE, Yogev L, Hauser R, Botchan A, Yavetz H. 2000. Maturation phenotype of Sertoli cells in testicular biopsies of azoospermic men. Human Reproduction 15: 1537-1542. https://doi.org/10.1093/humrep/15.7.1537
  13. McCluggage WG, Shanks JH, Whiteside C, Maxwell P, Banerjee SS, Biggart JD. 1998. Immunohistochemical study of testicular sex cord-stromal tumors, including staining with anti-inhibin antibody. Am J Surg Pathol 22: 615-619. https://doi.org/10.1097/00000478-199805000-00013
  14. McLachlan NJ, Kennedy PC. 2002. Tumors of the genital system. pp. 547-573. In: Meuten DJ(ed.). Tumors in Domestic Animals. 4th ed. Blackwell. Iowa state press. Ames.
  15. Meyts RD. 2007. Recent advances and future directions in research on testicular germ cell cancer. Int J Androl 30: 192-197. https://doi.org/10.1111/j.1365-2605.2007.00810.x
  16. Peters M, Teerds K, Van Der Gaag I, De Rooij D, van Sluijs F. 2001. Use of antibodies against LH receptor, 3beta-hydroxysteroid dehydrogenase and vimentin to characterize different types of testicular tumour in dogs. Reproduction 121: 287-296. https://doi.org/10.1530/rep.0.1210287
  17. Rogatsch H, Jezek D, Hittmair A, Mikuz G, Feichtinger H. 1996. Expression of vimentin, cytokeratin, and desmin in Sertoli cells of human fetal, cryptorchid, and tumour-adjacent testicular tissue. Virchows Archiv 427: 497-502.
  18. Rosai J. 2004. Rosai and Ackerman's Surgical Pathology. 4th ed. pp. 1412-1456. Mosby, Edinburgh.
  19. Sato K, Ueda Y, Sakurai A, Ishikawa Y, Okamoto S, Ikawa H, Katsuda S. 2005. Large cell calcifying Sertoli cell tumor of the testis: comparative immunohistochemical study with Leydig cell tumor. Pathol Int 55: 366-371. https://doi.org/10.1111/j.1440-1827.2005.01838.x
  20. Shiras A, Shepal V, Bhonde M, Shastry P. 2001. S100 expression in primary mouse fibroblast cultures. In Vitro Cell Dev Biol Anim 37: 172-174. https://doi.org/10.1290/1071-2690(2001)037<0172:SEIPMF>2.0.CO;2
  21. Stewart CJR, Nandini CL, Richmond JA. 2000. Value of A103(melan-A) immunostaining in the differential diagnosis of avarian sex cord stromal tumours. J Clin Pathol 53: 206-211. https://doi.org/10.1136/jcp.53.3.206
  22. Stosiek P, Kasper M, Karsten U. 1990. Expression of cytokeratins 8 and 18 in human Sertoli cells of immature and atrophic seminiferous tubules. Differntiation 43: 66-70. https://doi.org/10.1111/j.1432-0436.1990.tb00431.x
  23. Sung MT, Jones TD, Beck SD, Foster RS, Cheng L. 2006. OCT4 is superior to CD30 in the diagnosis of metastatic embryonal carcinomas after chemotherapy. Hum Pathol 37: 662-667. https://doi.org/10.1016/j.humpath.2006.01.019
  24. Tanaka Y, Carney JA, Ijiri R, Kato K, Miyake T, Nakatani Y, Misugi K. 2002. Utility of immunostaining for S-100 protein subunits in gonadal sex cord-stromal tumors, with emphasis on the large-cell calcifying Sertoli cell tumor of the testis. Hum Pathol 33: 285-289. https://doi.org/10.1053/hupa.2002.32224
  25. Wilhelm D, Palmer S, Koopman P. 2007. Sex determination and gonadal development in mammals. Physiol Rev 87: 1-28. https://doi.org/10.1152/physrev.00009.2006
  26. Yu CH, Hwang DN, Yhee JY, Kim JH, Im KS, Nho WG, Lyoo YS, Sur JH. 2009. Comparative immunohistochemical characterization of canine seminomas and Sertoli cell tumors. J Vet Sci 10: 1-7. https://doi.org/10.4142/jvs.2009.10.1.1