Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Comparison of polymerase chain reaction for antigen receptor gene rearrangement and flow cytometric analysis for the diagnosis of canine lymphoma

  • Song, Ru-Hui (College of Veterinary Medicine, Chonbuk National University) ;
  • Yu, Do-Hyeon (College of Veterinary Medicine, University of Missouri) ;
  • Kim, Jun-Hwan (College of Veterinary Medicine, Chonbuk National University) ;
  • Lee, Hyun-Seok (College of Veterinary Medicine, Chonbuk National University) ;
  • Lee, Da-Mi (College of Veterinary Medicine, Chonbuk National University) ;
  • Park, Chul (College of Veterinary Medicine, Chonbuk National University) ;
  • Yu, Il-Jung (College of Veterinary Medicine, Chonbuk National University) ;
  • Park, Jin-Ho (College of Veterinary Medicine, Chonbuk National University)
  • Received : 2011.07.29
  • Accepted : 2011.09.02
  • Published : 2011.09.30
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Abstract

Lymphoma is the most common hematopoietic malignancy in dogs. Diagnosis of lymphoma is classically performed by morphological assessment and immunohistochemistry. But some cases in the early stage are difficult to distinguish and need more objective and accurate methods. So, Polymerase chain reaction (PCR) for antigen receptor rearrangements (PARR) and flow cytometric immunophenotype of lymphoma have been developed continuously. In this study, we performed these two methods to classify lymphoma type in 3 cases. According to PARR analysis, B cell origin lymphoma was diagnosed in two of three cases by testing PBMC and lymph node. All fine needle aspiration (FNA) samples of lymph nodes had high expression of CD21 on >88% of total cell population and PBMC samples also showed high expression of CD21 on >30% of total lymphocytes in those two cases, while the expression of CD3, CD4 and CD8 was absent. These results suggest that concurrent use of PARR and flow cytometric immunophenotype is more effective and valuable tool for the diagnosis and monitoring of canine lymphoma patients.

Keywords

References

  1. Bahloul M, Asnafi V, Macintyre E. 2005. Clinical impact of molecular diagnostics in low-grade lymphoma. Best Pract Res Clin Haematol 18: 97-111. https://doi.org/10.1016/j.beha.2004.08.005
  2. Bartolo C, Viswanatha DS. 2001. Molecular methods for minimal residual disease detection in hematolymphoid diseases: principles and applications. J Clini Ligand Assay 24:76-93.
  3. Burnett RC, Vernau W, Modiano JF, Olver CS, Moore PF, Avery AC. 2003. Diagnosis of canine lymphoid neoplasia using clonal rearrangements of antigen receptor genes. Vet Pathol 40: 32-41. https://doi.org/10.1354/vp.40-1-32
  4. Byrne KM, Kim HW, Chew BP, Reinhart GA, Hayek MG. 2000. A standardized gating technique for the generation of flow cytometry data for normal canine and normal feline blood lymphocytes. Vet Immunol Immunopathol 73: 167-182. https://doi.org/10.1016/S0165-2427(99)00163-4
  5. Cobbold S, Metcalfe S. 1994. Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW). Tissue Antigens 43: 137-154. https://doi.org/10.1111/j.1399-0039.1994.tb02315.x
  6. Comazzi S, Gelain ME. 2011. Use of flow cytometric immunophenotyping to refine the cytological diagnosis of canine lymphoma. Vet J 188: 149-155. https://doi.org/10.1016/j.tvjl.2010.03.011
  7. Culmsee K, Nolte I. 2002. Flow cytometry and its application in small animal oncology. Methods Cell Sci 24: 49-54. https://doi.org/10.1023/A:1024133628927
  8. Culmsee K, Simon D, Mischke R, Nolte I. 2001. Possibilities of flow cytometric analysis for immunophenotypic characterization of canine lymphoma. J Vet Med A Physiol Pathol Clin Med 48: 199-206. https://doi.org/10.1046/j.1439-0442.2001.00351.x
  9. Das DK. 2006. Serous effusions in malignant lymphomas: a review. Diagn Cytopathol 34: 335-347. https://doi.org/10.1002/dc.20432
  10. Dorn CR, Taylor DO, Hibbard HH. 1967. Epizootiologic characteristics of canine and feline leukemia and lymphoma. Am J Vet Res 28: 993-1001.
  11. Dunphy CH, Ramos R. 1997. Combining fine-needle aspiration and flow cytometric immunophenotyping in evaluation of nodal and extranodal sites for possible lymphoma: a retrospective review. Diagn Cytopathol 16: 200-206. https://doi.org/10.1002/(SICI)1097-0339(199703)16:3<200::AID-DC2>3.0.CO;2-J
  12. Fend F, Quintanilla-Martinez L, Kumar S, Beaty MW, Blum L, Sorbara L, Jaffe ES, Raffeld M. 1999. Composite low grade B-cell lymphomas with two immunophenotypically distinct cell populations are true biclonal lymphomas. A molecular analysis using laser capture microdissection. Am J Pathol 154: 1857-1866. https://doi.org/10.1016/S0002-9440(10)65443-0
  13. Gibson D, Aubert I, Woods JP, Abrams-Ogg A, Kruth S, Wood RD, Bienzle D. 2004. Flow cytometric immunophenotype of canine lymph node aspirates. J Vet Intern Med 18: 710-717. https://doi.org/10.1111/j.1939-1676.2004.tb02610.x
  14. Greenlee PG, Filippa DA, Quimby FW, Patnaik AK, Calvano SE, Matus RE, Kimmel M, Hurvitz AI, Lieberman PH. 1990. Lymphomas in dogs. A morphologic, immunologic, and clinical study. Cancer 66: 480-490. https://doi.org/10.1002/1097-0142(19900801)66:3<480::AID-CNCR2820660314>3.0.CO;2-X
  15. Grindem CB. 1996. Blood cell markers. Vet Clin North Am Small Anim Pract 26: 1043-1064. https://doi.org/10.1016/S0195-5616(96)50055-0
  16. Keller RL, Avery AC, Burnett RC, Walton JA, Olver CS. 2004. Detection of neoplastic lymphocytes in peripheral blood of dogs with lymphoma by polymerase chain reaction for antigen receptor gene rearrangement. Vet Clin Pathol 33: 145-149. https://doi.org/10.1111/j.1939-165X.2004.tb00364.x
  17. Kiupel M, Teske E, Bostock D. 1999. Prognostic factors for treated canine malignant lymphoma. Vet Pathol 36: 292-300. https://doi.org/10.1354/vp.36-4-292
  18. Lana SE, Jackson TL, Burnett RC, Morley PS, Avery AC. 2006. Utility of polymerase chain reaction for analysis of antigen receptor rearrangement in staging and predicting prognosis in dogs with lymphoma. J Vet Intern Med 20: 329-334. https://doi.org/10.1111/j.1939-1676.2006.tb02864.x
  19. Mitterbauer-Hohendanner G, Mannhalter C, Winkler K, Mitterbauer M, Skrabs C, Chott A, Simonitsch-Klupp I, Gleiss A, Lechner K, Jaeger U. 2004. Prognostic significance of molecular staging by PCR-amplification of immunoglobulin gene rearrangements in diffuse large B-cell lymphoma (DLBCL). Leukemia 18: 1102-1107. https://doi.org/10.1038/sj.leu.2403376
  20. Moore PF, Vernau W. 2000. Lymphocytes: differentiation molecules in diagnosis and prognosis. pp. 247-255. In: Feldman BF, Zinkl JG, Schalm OW(ed.). Schalm's veterinary hematology. Lippincott Williams & Wilkins, Philadelphia.
  21. Morley A. 1998. Quantifying leukemia. N Engl J Med 339: 627-629. https://doi.org/10.1056/NEJM199808273390911
  22. Poljak M, Barlic J, Seme K, Avsic-Zupanc T, Zore A. 1995. Isolation of DNA from archival Papanicolaou stained cytological smears using a simple salting-out procedure. Clin Mol Pathol 48: M55-56. https://doi.org/10.1136/mp.48.1.M55
  23. Qualman SJ, France M, Grizzle WE, LiVolsi VA, Moskaluk CA, Ramirez NC, Washington MK. 2004. Establishing a tumour bank: banking, informatics and ethics. Br J Cancer 90: 1115-1119. https://doi.org/10.1038/sj.bjc.6601678
  24. Raulet DH. 1989. The structure, function, and molecular genetics of the gamma/delta T cell receptor. Annu Rev Immunol 7: 175-207. https://doi.org/10.1146/annurev.iy.07.040189.001135
  25. Rothe G, Schmitz G. 1996. Consensus protocol for the flow cytometric immunophenotyping of hematopoietic malignancies. Working Group on Flow Cytometry and Image Analysis. Leukemia 10: 877-895.
  26. Ruslander DA, Gebhard DH, Tompkins MB, Grindem CB, Page RL. 1997. Immunophenotypic characterization of canine lymphoproliferative disorders. In Vivo 11: 169-172.
  27. Sandhaus LM. 2000. Fine-needle aspiration cytology in the diagnosis of lymphoma. The next step. Am J Clin Pathol 113: 623-627. https://doi.org/10.1309/BEBH-BLA2-J5AY-YE2L
  28. Schatz DG, Oettinger MA, Schlissel MS. 1992. V (D) J recombination: molecular biology and regulation. Annu Rev Immunol 10: 359-383. https://doi.org/10.1146/annurev.iy.10.040192.002043
  29. Tai YC, Peh SC. 2003. Immunoglobulin gene rearrangement of lymphoid malignancies : Application of the PCR technique to formalin-fixed, paraffin-embedded tissues. J Clin Exp Hematopathol 43: 1-10. https://doi.org/10.3960/jslrt.43.1
  30. Tamura K, Yagihara H, Isotani M, Ono K, Washizu T, Bonkobara M. 2006. Development of the polymerase chain reaction assay based on the canine genome database for detection of monoclonality in B cell lymphoma. Vet Immunol Immunopathol 110: 163-167. https://doi.org/10.1016/j.vetimm.2005.10.009
  31. Vail DM, Young KM. 2007. Canine lymphoma and lymphoid leukemia. pp. 699-733. In: Withrow SJ, Vail DM(ed.). Withrow and MacEwen's Small Animal Clinical Oncology. 4th ed. Saunders Elsevier, Missouri.
  32. van Dongen JJ, Langerak AW, Bruggemann M, Evans PA, Hummel M, Lavender FL, Delabesse E, Davi F, Schuuring E, Garcia-Sanz R, van Krieken JH, Droese J, Gonzalez D, Bastard C, White HE, Spaargaren M, Gonzalez M, Parreira A, Smith JL, Morgan GJ, Kneba M, Macintyre EA. 2003. Design and standardization of PCR primers and protocols for detection of clonal immunoglobulin and T-cell receptor gene recombinations in suspect lymphoproliferations: report of the BIOMED-2 Concerted Action BMH4-CT98-3936. Leukemia 17: 2257-2317. https://doi.org/10.1038/sj.leu.2403202
  33. Vernau W, Moore PF. 1999. An immunophenotypic study of canine leukemias and preliminary assessment of clonality by polymerase chain reaction. Vet Immunol Immunopathol 69: 145-164. https://doi.org/10.1016/S0165-2427(99)00051-3
  34. Vince A, Poljak M, Seme K. 1998. DNA extraction from archival Giemsa-stained bone-marrow slides: comparison of six rapid methods. Br J Haematol 101: 349-351. https://doi.org/10.1046/j.1365-2141.1998.00702.x
  35. Wan JH, Trainor KJ, Brisco MJ, Morley AA. 1990. Monoclonality in B cell lymphoma detected in paraffin wax embedded sections using the polymerase chain reaction. J Clin Pathol 43: 888-890. https://doi.org/10.1136/jcp.43.11.888
  36. Weiss DJ. 2002. Application of flow cytometric techniques to veterinary clinical hematology. Vet Clin Pathol 31: 72-82. https://doi.org/10.1111/j.1939-165X.2002.tb00284.x
  37. Xu D, Du J, Kamino H, Ratech H. 2004. Rapid diagnosis of clonal immunoglobulin heavy chain gene rearrangements in cutaneous B-cell lymphomas using the LightCycler-Polymerase Chain Reaction with DNA melting curve analysis. Am J Dermatopathol 26: 385-389. https://doi.org/10.1097/00000372-200410000-00007
  38. Yagihara H, Tamura K, Isotani M, Ono K, Washizu T, Bonkobara M. 2007. Genomic organization of the T-cell receptor gamma gene and PCR detection of its clonal rearrangement in canine T-cell lymphoma/leukemia. Vet Immunol Immunopathol 115: 375-382. https://doi.org/10.1016/j.vetimm.2006.11.005
  39. Yu DH, Li YH, Lee JH, Noh DH, Song RH, Lee MJ, Choi US, Park JH. 2009. Detection of canine lymphoma by the amplification of antigen receptor gene rearrangements. J Vet Clin 26: 419-422.