Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Effect of Arresting MCF-7 Human Breast Carcinoma Cell at G2/M Phase of Trichosanthes Kirilowii

천화분이 MCF-7 유방암 세포주의 G2/M 세포주기 억제에 미치는 영향

  • Jeong, Seung-Min (Department of Orinetal Internal Medicine, College of Oriental Medicine, Kyungwon University) ;
  • Jeong, Mi-Kyung (Department of Orinetal Internal Medicine, College of Oriental Medicine, Kyungwon University) ;
  • Ko, Seong-Gyu (Department of Preventive Medicine, College of Oriental Medicine, Kyung-Hee University) ;
  • Choi, You-Kyung (Department of Orinetal Internal Medicine, College of Oriental Medicine, Kyungwon University) ;
  • Park, Jong-Hyeong (Department of Orinetal Internal Medicine, College of Oriental Medicine, Kyungwon University) ;
  • Jun, Chan-Yong (Department of Orinetal Internal Medicine, College of Oriental Medicine, Kyungwon University)
  • 정승민 (경원대학교 한의과대학 내과학교실) ;
  • 정미경 (경원대학교 한의과대학 내과학교실) ;
  • 고성규 (경희대학교 한의과대학 예방의학교실) ;
  • 최유경 (경원대학교 한의과대학 내과학교실) ;
  • 박종형 (경원대학교 한의과대학 내과학교실) ;
  • 전찬용 (경원대학교 한의과대학 내과학교실)
  • Received : 2011.07.20
  • Accepted : 2011.08.26
  • Published : 2011.10.25
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Abstract

The purpose of this study is to investigate the anti-proliferative mechanism by Trichosanthes kirilowii (TCK) in MCF-7 human breast carcinoma cell. In this study, we used human breast cancer cell line, Michigan cancer foundation-7 cells (MCF-7 cells). They were co-incubated with 30~200 ${\mu}g$/ml TCK for 48 hours, and cell viability was measured by Water-soluble tetrazolium salt-1 (WST-1) assay. After MCF-7 cells were exposed to 60 ${\mu}g$/ml of TCK for 0, 3, 6, 12, 24, 48 hours, We performed flow analysis cytometry sorting(FACS) and western blot analysis. We investigated the effect of dose-dependent cell growth inhibition by TCK, which could be proved by WST-1 assay. Also, flow cytometry analysis showed that TCK increased percentage of subG1 phase and G2/M phase cell cycle. In addition, TCK induced apoptosis through the expression of caspase-9, -3 and poly(ADP-ribose) polymerase(PARP) activation. Moreover, we showed that ATM-dependent G2/M phase arrest by DNA damage and phosphorylation of chk2, cdc25C, cdc2(Tyr15). Taken together, these results suggest that by G2/M phase arrest through DNA damage and inducing of apoptosis through intrinsic pathway, TCK may have potential tumor suppressor in breast cancer.

Keywords

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