동의생리병리학회지 (Journal of Physiology & Pathology in Korean Medicine)

  • 제25권5호
  • /
  • Pages.799-806
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  • 2011
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  • 1738-7698(pISSN)
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  • 2288-2529(eISSN)
대한동의생리학회·한의병리학회 ( The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine)
권호 표지

보양환오탕(補陽還五湯)이 흰쥐 뇌출혈 손상에 미치는 영향에 대한 면역조직화학 연구

Neuroprotective Effects of Boyanghwano-tang on Intracerebral Hemorrhage-Induced Rats Using Immunohistochemistry

  • 차재덕 (경희대학교 동서의학대학원 한의과학) ;
  • 이준석 (경희대학교 동서의학대학원 한의과학) ;
  • 신정원 (경희대학교 동서의학대학원 한의과학) ;
  • 김성준 (경희대학교 동서의학대학원 한의과학) ;
  • 강희 (경희대학교 동서의학대학원 한의과학) ;
  • 손낙원 (경희대학교 동서의학대학원 한의과학)
  • Cha, Jae-Deog (Division of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Lee, Joon-Suk (Division of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Shin, Jung-Won (Division of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Kim, Seong-Joon (Division of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Kang, Hee (Division of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Sohn, Nak-Won (Division of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung Hee University)
  • 투고 : 2011.09.02
  • 심사 : 2011.10.06
  • 발행 : 2011.10.25
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초록

This study was performed in order to evaluate the neuroprotective effect of Boyanghwano-tang (BYT) water extract on intracerebral hemorrhage (ICH). ICH was induced by the stereotaxic intrastriatal injection of bacterial collagenase type IV in Sprague-Dawley rats. BYT was orally given once a day for 3 days after ICH. Hematoma volume and percentage edema were examined. As imflammatory markers, myeloperoxidase (MPO)-positive neutrophils infiltration and iNOS expression in the peri-ICH regions were examined using immunohistochemistry. As cellular damage markers, c-Fos, Bax, and HSP72 positive cells in the peri-ICH regions were measured also. BYT significantly reduced the hematoma volume and percentage edema of the ICH-induced rat brain. In the peri-hematoma regions, BYT significantly reduced MPO-positive neutrophil infiltration and iNOS expression of the ICH-induced rat brain. Additionally, BYT significantly reduced c-Fos, Bax, and HSP72 positive cells in the peri-hematoma regions of the ICH-induced rat brain. These results suggest that BYT plays a neuroprotective role against ICH through suppression of inflammatory responses, apoptosis and cellular damage.

키워드

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