Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Isolation and Characterization of Lactic Acid Bacteria with Angiotensin-Converting Enzyme Inhibitory and Antioxidative Activities

안지오텐신 전환효소 저해 활성 및 항산화 활성을 가진 젖산균의 분리 및 특성

  • Park, Sung-Bo (College of Natural Resources and Life Science, Pusan National University) ;
  • Kim, Jeong-Do (College of Natural Resources and Life Science, Pusan National University) ;
  • Lee, Na-Ri (College of Natural Resources and Life Science, Pusan National University) ;
  • Jeong, Jin-Ha (College of Natural Resources and Life Science, Pusan National University) ;
  • Jeong, Seong-Yun (Department of Medical Life Science, Catholic University of Daegu) ;
  • Lee, Hee-Seob (Department of Food Science and Nutrition, Pusan National University) ;
  • Hwang, Dae-Youn (College of Natural Resources and Life Science, Pusan National University) ;
  • Lee, Jong-Sup (Sandong Processing Plant, Nonghyup) ;
  • Son, Hong-Joo (College of Natural Resources and Life Science, Pusan National University)
  • 박성보 (부산대학교 생명자원과학대학) ;
  • 김정도 (부산대학교 생명자원과학대학) ;
  • 이나리 (부산대학교 생명자원과학대학) ;
  • 정진하 (부산대학교 생명자원과학대학) ;
  • 정성윤 (대구가톨릭대 의생명과학과) ;
  • 이희섭 (부산대학교 식품영양학과) ;
  • 황대연 (부산대학교 생명자원과학대학) ;
  • 이종섭 (밀양 산동농협) ;
  • 손홍주 (부산대학교 생명자원과학대학)
  • Received : 2011.07.20
  • Accepted : 2011.10.24
  • Published : 2011.10.31
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Abstract

In this study, we isolated and characterized plant-associated lactic acid bacteria which are able to produce angiotensin-converting enzyme (ACE) inhibitory and antioxidative activities. Five lactic acid bacteria were isolated from plants (grape and leek), a plant-associated fermentative product (Kimchi) and Korean traditional alcohol (Dongdongju). Strains K-1 and K-21 from Kimchi, strain L-5 from leek, strain G-3 from grape, and strain D-3 from Dongdongju were identified as Pediococcus pentosaceus, Lactobacillus plantarum, Weissella cibaria, L. plantarum, and L. brevis, respectively, by 16S rRNA gene analysis. ACE inhibitory activities of isolated strains ranged from 44.3 to 71.9% in the MRS broth. G-3, L-5 and K-1 strains especially showed high ACE inhibitory activities (59.8-98.69%) in the MRS broth containing skim milk. DPPH radical scavenging activities of the strains were in the range of 42.5-82.7%. All strains showed varying levels of resistance in artificial gastric fluid (pH 2.5), retaining viability ranging from 42.2 to 88.1% after 3 hr of incubation. All strains showed high resistance to 0.3% oxgall after 24 hr of incubation; survival rates were in the range of 55.4-112.8%. Isolated strains were found to be antagonistic to some pathogens including Pseudomonas aeruginosa.

본 연구에서는 ACE 저해능 및 항산화능이 있는 식물성 젖산균을 다양한 식물체로부터 분리한 후, 그 특성을 조사하였다. 김치, 부추, 포도 및 동동주에서 K-1, K-21, L-5, G-3 및 D-3 균주가 분리되었으며, 16S rRNA gene 염기서열 분석을 통하여 이들은 각각 Pediococcus pentosaceus, Lactobacillus plantarum, Weissella cibaria, L. plantarum 및 L. brevis로 동정되었다. 분리균주들은 MRS broth에서 44.3-71.9%의 ACE 저해능을 나타내었으며, 특히 G-3, L-5, K-1 균주는 skim milk가 함유된 MRS broth에서 59.0-8-98.6%의 높은 ACE 저해능을 나타내었다. 분리 균주는 42.5-82.7%의 DPPH radical 소거능을 나타내었으며, G-3 및 K-1 균주는 pH 2.5의 인공위액에서 42.2-88.1%의 높은 생존율을 나타내었다. 분리균주는 0.3% oxgall에서 24시간 배양시 55.4-112.8%의 내성을 나타내었다. 또한 분리균주는 유기산 생성에 따른 pH 감소 효과로 인하여 Pseudomonas aeruginosa를 포함한 일부 병원성 세균의 생육을 억제할 수 있었다.

Keywords

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