Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
권호 표지
DOI QR코드

DOI QR Code

Antithrombotic and Antiplatelet Activity of Extract from Prunella vulgaris

하고초 추출물의 항혈전 효능 및 혈소판 응집 억제작용

  • Yang, Won-Kyung (Center of Herbal Resources Research, Korea Institute of Oriental Medicine) ;
  • Sung, Yoon-Young (Center of Herbal Resources Research, Korea Institute of Oriental Medicine) ;
  • Kim, Ho-Kyoung (Center of Herbal Resources Research, Korea Institute of Oriental Medicine)
  • 양원경 (한국한의학연구원 한약자원연구센터) ;
  • 성윤영 (한국한의학연구원 한약자원연구센터) ;
  • 김호경 (한국한의학연구원 한약자원연구센터)
  • Received : 2011.07.13
  • Accepted : 2011.10.19
  • Published : 2011.10.31
Download PDF
⟨ Previous Next ⟩

Abstract

This study was performed to develop effective antithrombotic agents from traditional herb extracts. Prunella vulgaris L. has been used traditionally as a medical resource in cancer therapy, as well as treatment of hypertension and inflammation, and as a diuretic. However, the effects of Prunella vulgaris on thrombosis and platelet activation have not been clearly understood. Antithrombotic and antiplatelet activities of oriental medicinal herbs were investigated by evaluating the effect of the aqueous extract from Prunella vulgaris on the blood coagulation, platelet aggregation and fibrinolysis. Prunella vulgaris extracts showed effective anticoagulant activity in coagulation times such as activated partial thromboplastin time (APTT) and prothrombin time (PT). Prunella vulgaris also inhibited adenosine diphosphate (ADP)- and collagen-induced platelet aggregation. In addition, evaluation of fibrinolytic activity showed that the Prunella vulgaris extracts have high solubility. From these results, it is suggested that Prunella vulgaris can be a potential candidate for anticoagulants and antiplatelets, as well as fibrinolytic agents.

한방생약제의 항혈전 및 혈소판 응집 억제 효능을 탐색하기 위하여 하고초의 물 추출물로 혈전 용해능 활성과 혈액 응고시간 지연효과 즉 PT (prothrombin time), APTT (activated partial thromboplastin time)와 혈소판 응집억제 활성 등에 대해 항혈전 효능을 평가하였다. 혈전용해도를 측정하는 fibrin plate가 용해되어 형성된 투명환의 넓이를 측정하는 실험을 진행한 결과 혈전용해도가 농도의존적으로 효능을 나타내었다. 혈액 응고 cascade에 미치는 영향을 알아보기 위해 혈액 응고 시간 지연 및 단축 효과를 확인하고자 APTT와 PT에 미치는 영향을 조사한 결과 PT의 경우 10 mg/ml, 5 mg/ml의 경우에는 대조군보다 우수한 지연효과를 보였다. APTT의 경우에는 10 mg/ml, 5 mg/ml는 대조군과 비교하여 매우 탁월한 지연효과를 보이고, 2.5 mg/ml, 1.25 mg/ml에서도 높은 지연효과를 나타냈다. 혈소판의 응집에 따라 형성되는 두 전극 사이에 형성된 전기적 저항의 변화로 나타나는 실험을 시행한 결과 하고초의 ADP와 collagen에서 뛰어난 응집억제 활성을 보였다. 따라서 위의 항혈전 효능평가 실험결과를 볼 때 하고초를 향후에 혈전 질환의 치료제 개발에 효과적으로 이용될 수 있을 것으로 사료된다.

Keywords

References

  1. Au, T. K., T. L. Lam, T. B. Ng, W. P. Fong, and D. C. Wan. 2001. A comparison of HIV-1 integrase inhibition by aqueous and methanol extracts of Chinese medicinal herbs. J. Life Sci. 68, 1687-1694. https://doi.org/10.1016/S0024-3205(01)00945-6
  2. Birk, S., C. Kruuse, and K. A. Peter. 2006. The headache-inducing effect of cilostazol in human volunteers. Cephalagia 26, 1304-1309. https://doi.org/10.1111/j.1468-2982.2006.01218.x
  3. De Meyer, S. F., K. Vanhooelbbeke, and K. l. Broos. 2008. Antiplatelet drugs. British J. Haematology 142, 515-528. https://doi.org/10.1111/j.1365-2141.2008.07233.x
  4. Fan, J., Y. Zhang, X. Chang, B. Zhang, D. Jiang, M. Saito, and Z. Li. 2009. Antithrombotic and fibrinolytic activities of methanolic extract of aged sorghum vinegar. J. Agric. Food Chem. 57, 8683-8687. https://doi.org/10.1021/jf901680y
  5. Francescone, S. and J. L. Halperin. 2008. "Triple theraphy" or triple threat?: balancing the risks of antithrombotic theraphy for patients with atrial fibrillation and coronary stents. J. Am. Coll. Cardiol. 51, 826-827. https://doi.org/10.1016/j.jacc.2007.11.034
  6. Fontanay, S., M. Grare, J. Mayer, C. Finance, and R. E. Duval. 2008. Ursolic, oleanolic and betulinic acids: Antibacterial spectra and selectivity indexs. J. Ethnopharmacol. 120, 272-276. https://doi.org/10.1016/j.jep.2008.09.001
  7. Johnson, S. 2008. Known knowns and known unknowns: Risks associated with combination antithrombotic theraphy. Thromb. Res. 23, S7-S11.
  8. Jung, Y. B., K. J. Roh, J. A. Jung, K. Jung, H. Yoo, Y. B. Cho, W. J. Kwak, D. K. Kim, K. H. Kim, and C. K. Han. 2001. Effect of SKI 306X, a new herbal anti-arthritic agent, in patients with osteoarthritis of the knee: a double-blind placebo controlled study. Am. J. Chin. Med. 29, 485-491. https://doi.org/10.1142/S0192415X01000502
  9. Kageyama, S., M. Kurokawa, and K. Shiraki. 2000. Extract of Prunella vulgaris spikes inhibits HIV replication at reverse transcription in vitro and can be absorbed from intes-tine in vivo. Chem. Chemother.11, 157-164.
  10. Kim, Y. W. and D. C. Kim. 1999. Evaluation of thrombolytic effect of streptokinase-dextran conjugate in a rat model of arterial thrombosis. J. Korean Pharm. Sci. 29, 211-216.
  11. Kojima, H. and H. Ogura. 1986. Triterpenoids from Prunella vulgaris. Phytochemistry 25, 729-733. https://doi.org/10.1016/0031-9422(86)88033-5
  12. Lam, T. L., M. L. Lam, T. K. Au, D. T. Ip, T. B. Ng, W. P. Fong, and D. C. Wan. 2002. A comparison of human immunodeficiency virus type-1 protease inhibition activities by the aqueous and methanol extracts of Chinese medicinal herbs. J. Life Sci. 67, 2889-2896.
  13. Lamaison, J. L., C. Petitjean-Freytet, and A. Carnat. 1991. Medicinal lamiaceae with antioxidant properties, a potential source of rosmarinic acid. Pharm. Acta. Helv. 66, 185-188.
  14. Lee, H. and J. Y. Lin. 1988. Anti mutagenic activity of extracts from anticancer drugs in medicine. Mutat. Res. 204, 229-234. https://doi.org/10.1016/0165-1218(88)90093-6
  15. Lee, H. S. 1995. How safe is the readministration of streptokinase? Drug Safety 13, 76-80. https://doi.org/10.2165/00002018-199513020-00002
  16. Liu, F. and T. B. Ng. 2000. Anti oxidative and free radical scavenging activities of selected medicinal herbs. J. Life Sci. 166, 725-735.
  17. Liu, J. 1995. Pharmacology of oleanolic acid and ursolic acid. J. Ethnopharmacol. 49, 57-68. https://doi.org/10.1016/0378-8741(95)90032-2
  18. Liu, S., S. Jiang, Z. Wu, L. Lv, J. Zhang, Z. Zhu, and S. Wu. 2002. Identification of inhibitors of the HIV-1 gp41 six-helix bundle formation from extracts of Chinese medicinal herbs Prunella vulgaris and Rhizoma cibotte. Life Sci. 71, 1779-1791. https://doi.org/10.1016/S0024-3205(02)01939-2
  19. Ryu, S. Y., M. H. Oak, S. K. Yoon, D. I. Cho, G. S. Yoo, T. S. Kim, and K. M. Kim. 2000. Anti-allergic and anti-inflammatory triterpenes from the herb of Prunella vulgaris. Planta Med. 66, 358-360. https://doi.org/10.1055/s-2000-8531
  20. Samama, C. M. and N. Rosencher. 2009. New oral anticoagulant agent: do not walk out of the line. Annales Francaises d'Anesthesie et de Reanimation 28, 836-837. https://doi.org/10.1016/j.annfar.2009.08.005
  21. Shimano, T., M. Mizuno, H. Okamoto, and I. Adachi. 1956. Studies on triterpenoids. Ⅸ. On the new component of Prunella, ursolic acid. J Pharm Soc Jpn 76, 974-975.
  22. Sohn, H. Y., K. H. Son, C. S. Kwon, and G. S. Kwon. 2007. A compound comprising 2α-hydroxy-oleanolic acid for prevention and control of thrombosis. Korea patent 1007040030000.
  23. Tanachatchairatana, T., J. B. Bremner, R. Chokchaisiri, and A. Suksamrarn. 2008. Antimycobacterial activity of cinnamate-based esters of the triterpenes betulinic, oleanolic and ursolic acids. Chem. Pharm. Bull. 56, 194-198. https://doi.org/10.1248/cpb.56.194
  24. Xu, H. X., S. H. Lee, S. F. Lee, R. L White, and J. Blay. 1999. Isolation and characterization of an anti-HSV polysaccharide from Prunella vulgaris. Antiviral Res. 44, 43-54. https://doi.org/10.1016/S0166-3542(99)00053-4
  25. Yang, S. A., N. K. Im, and I. S. Lee. 2007. Effect of methanolic extract from salvia miltiorrhiza Bunge on in vitro antithrombotic and antioxidative activities. Korean J. Food Sci. Technol. 39, 83-87.
  26. Yim, E. K., M. J. Lee, K. H. Lee, S. J. Um, and J. S. Park. 2006. Antiproliferative and antiviral mechanisms of ursolic acid and dexamethasone in cervical carcinomacell lines. Int. J. Gynecol. Cancer 16, 2023-2031. https://doi.org/10.1111/j.1525-1438.2006.00726.x

Cited by

  1. The protective effect of Prunella vulgaris ethanol extract against vascular inflammation in TNF-α-stimulated human aortic smooth muscle cells vol.46, pp.7, 2013, https://doi.org/10.5483/BMBRep.2013.46.7.214