Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Ethyl Acetate Extract of Korean Rice Wine Lees Inhibits IgE-Mediated Degranulation in Rat Basophilic Leukemia RBL-2H3 Cells and Passive Cutaneous Anaphylaxis in Mice

주박 에틸아세테이트 추출물의 항알러지 효과

  • Kang, Yeo-Jin (Division of Biological Science and Technology, College of Science and Technology, Yonsei University) ;
  • Park, Sae-Jin (Division of Biological Science and Technology, College of Science and Technology, Yonsei University) ;
  • Bae, Ki-Ho (Division of Biological Science and Technology, College of Science and Technology, Yonsei University) ;
  • Yoo, Jung-Min (R&D Center, Hanbul Cosmetics Co.) ;
  • Pyo, Hyeong-Bae (R&D Center, Hanbul Cosmetics Co.) ;
  • Choi, Ji-Ho (Rural Development Administiration, Fermention & Food Processing Division) ;
  • Kim, Tack-Joong (Division of Biological Science and Technology, College of Science and Technology, Yonsei University)
  • 강여진 (연세대학교 과학기술대학 생명과학기술학부) ;
  • 박세진 (연세대학교 과학기술대학 생명과학기술학부) ;
  • 배기호 (연세대학교 과학기술대학 생명과학기술학부) ;
  • 유정민 (한불화장품 기술연구소) ;
  • 표형배 (한불화장품 기술연구소) ;
  • 최지호 (농촌진흥청 발효이용과) ;
  • 김택중 (연세대학교 과학기술대학 생명과학기술학부)
  • Received : 2011.09.15
  • Accepted : 2011.10.26
  • Published : 2011.10.31
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Abstract

Mast cells, the central effector cells involved in the allergic response, release histamine, arachidonic acid, and proinflammatory cytokines. We investigated the effect of the ethyl acetate fraction (EA), derived from Korean rice wine lees, on RBL-2H3 cell degranulation and passive cutaneous anaphylaxis in an animal model. The EA fraction suppressed the release of beta-hexosaminidase, a marker of degranulation, and the mRNA expression of interleukin-3 (IL-3) and IL-13. EA also successfully suppressed the passive cutaneous anaphylaxis (PCA) reaction in mice in a dose-dependent manner. These results suggest that EA can inhibit mast cell degranulation through the inhibition of IL-3 and IL-13 mRNA expression, and that EA may potentially serve as an anti-allergic agent.

제 1형 과민반응은 비만세포에 의해 매개되는 알러지 반응으로서, 예기치 못한 알러젠에 의해 비만세포가 활성화 되어 탈 과립 되면서 히스타민등과 같은 여러 가지 매개체들을 분비하면서 과도하게 면역반응을 유도하게 된다. 주박(Korean rice wine lees)은 청주, 약주 등의 술을 걸러내는 과정에서 생기는 양조 부산물로서, 최근들어 청주를 걸러내는 과정에서 생기는 술 지게미의 생리기능에 대한 연구가 활발하게 진행되고 있다. 본 연구에서는 주박의 ethyl acetate (EA) 추출물을 이용하여 비만세포에서의 탈과립 억제 효과를 측정하였다. 그 결과 주박 EA 추출물이 탈과립의 표지인 ${\beta}$-hexosaminidase의 양을 농도 의존적으로 억제하는 것을 관찰하였고, 이러한 탈과립억제 현상이 다양한 사이토카인 중 IL-3과 IL-13의 양을 감소시킴으로써 매개된다는 사실을 RT-PCR을 통해 확인하였다. 또한 쥐에서 passive cutaneous anaphylaxis (PCA)를 유도하여 EA를 경구투여 했을 때 anaphylactic 증상을 효과적으로 억제하는 것을 관찰하였다. 이러한 결과들은 주박 EA 추출물이 항 알러지 약물의 개발에 잠재적인 후보물질이 될 수 있음을 시사한다.

Keywords

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