Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Isolation and Culture Properties of a Thermophilic Agarase-Producing Strain, Microbulbifer sp. SD-1

  • Kim, Do-Kyun (Department of Life Science and Biotechnology, Dongeui University) ;
  • Jang, Yu-Ri (Department of Life Science and Biotechnology, Dongeui University) ;
  • Kim, Kyoung-Hoon (Department of Life Science and Biotechnology, Dongeui University) ;
  • Lee, Mi-Nan (Department of Life Science and Biotechnology, Dongeui University) ;
  • Kim, A-Ra (Department of Life Science and Biotechnology, Dongeui University) ;
  • Jo, Eun-Ji (Department of Life Science and Biotechnology, Dongeui University) ;
  • Byun, Tae-Hwan (Department of Life Science and Biotechnology, Dongeui University) ;
  • Jeong, Eun-Tak (SNTECH Co., Ltd.) ;
  • Kwon, Hyun-Ju (Department of Life Science and Biotechnology, Dongeui University) ;
  • Kim, Byung-Woo (Department of Life Science and Biotechnology, Dongeui University) ;
  • Lee, Eun-Woo (Department of Life Science and Biotechnology, Dongeui University)
  • Received : 2011.07.06
  • Accepted : 2011.08.10
  • Published : 2011.09.30
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Abstract

An agar-degrading enzyme-producing strain was isolated from seawater. The isolate was identified as Microbulbifer sp. SD-1 by 16S rRNA sequencing analysis. The optimal pH and temperature for growth were 6.0 and $30^{\circ}C$, respectively, and growth was possible at pH 9.0 and $60^{\circ}C$. The isolate required 5% NaCl for optimal growth and showed 45% growth activity without NaCl. Agar concentrations of 0-0.4% in the medium did not affect growth. Thin-layer chromatography analysis revealed that this strain could degrade agar into a monosaccharide and oligosaccharide, which may have industrial applications.

Keywords

References

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