Biomolecules & Therapeutics

  • 제19권4호
  • /
  • Pages.487-492
  • /
  • 2011
  • /
  • 1976-9148(pISSN)
  • /
  • 2005-4483(eISSN)
한국응용약물학회 (The Korean Society of Applied Pharmacology)
권호 표지
DOI QR코드

DOI QR Code

Effects of Chenopodium album Linne on Gastritis and Gastric Cancer Cell Growth

  • 투고 : 2011.08.24
  • 심사 : 2011.10.05
  • 발행 : 2011.10.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

In our previous study, we investigated Chenopodium album Linne (CAL) ethanol extract and its fractions on anti-gastritic actions using the HCl/ethanol and indomethacin induced gastric lesion model and Helicobacter pylori (H. pylori). Based on the results, butanol fraction was most effective among fractions obtained from CAL. This study aims to elucidate the mechanisms of butanol fraction, and betaine as a constituent of the butanol fraction, on gastritis and anti-gastric cancer cell growth. First, we examined antioxidant properties using hydrogen peroxide and superoxide radical, and we found that butanol fraction and betaine may be good antioxidants. Second, cytotoxicity was assessed by measuring cell viability and 4,6-diamidino-2-phenylinodole dihydrochloride (DAPI) staining of human gastric cancer cells (AGS cells). We also examined the relationship between the cytotoxicity and intracellular $Ca^{2+}$ signaling mechanism. The butanol fraction demonstrated cell viability 71.49% at the concentration of 100 ${\mu}g/ml$ and increased intracellular $Ca^{2+}$ concentration in a dose dependent manner. Finally, we observed the mucus content as a defensive factor and gastric secretion as an aggressive factor, and found that the mucus content noticeably increased when treated with butanol fraction and betaine and gastric secretion decreased when treated with betaine in vivo study. From these results, we suggest that CAL butanol fraction and betaine may have protective effects on gastritis.

키워드

참고문헌

  1. Bessieres, M. A., Gibon, A. Y., Lefeuvre, J. C. and Larher, F. A. (1999) Single-step purifi cation for glycine betaine determination in plant extracts by isocratic HPLC. J. Agric. Food Chem. 47, 3718-3722. https://doi.org/10.1021/jf990031h
  2. Block, G. (1991) Vitamin C and cancer prevention: the epidemiologic evidence. Am. J. Clin. Nutr. 53, 270S-282S.
  3. Block, G., Henson, D. and Levine, M. (1991) Vitamin C: a new look. Ann. Intern. Med. 114, 909-910. https://doi.org/10.7326/0003-4819-114-10-909
  4. Choi, C. H., Cha, Y. J., An, C. S., Kim, K. J., Kim, K. C., Moon, S. P., Lee, Z. H. and Min, Y. D. (2004) Molecular mechanisms of heptaplatin effective against cisplatin-resistant cancer cell lines: less involvement of metallothionein. Cancer Cell Int. 4, 6-17. https://doi.org/10.1186/1475-2867-4-6
  5. Craig, S. A. (2004) Betaine in human nutrition. Am. J. Clin. Nutr. 80, 539-549.
  6. Dethloff, L. A., Patmore, S. J., Tierney, B. M., Bestervelt, L. L. and Zandee, J. C. (1998) Gastric effects of the CCK-B/gastrin receptor ligand CI-988 in cynomolgus monkeys. Food and Chemical Toxicology 36, 61-71. https://doi.org/10.1016/S0278-6915(97)00115-4
  7. llhami, G., Riad, E., Akcahan, G. and Laurent, B. (2006) Antioxidant activity of lignans from fringe tree (Chionanthus virginicus L.). European Food Research and Technology 223, 759-767. https://doi.org/10.1007/s00217-006-0265-5
  8. Janssens, G. P. J., De Rycke, H. D., Hesta, M. and De Wild., R. O. M. (1999) Analysis of carnitine, betaine, γ-butyrobetaine, and separate short-chain acylcarnitines in pigeon plasma, crop milk and tissues by HPLC coupled with UV-detection. Biotechnol. Techniq. 12, 231-234.
  9. Jeong, H. O., Suh, Y. S. and Chung, Y. J. (2010) Choline and betaine concentrations in breast milk of korean lactating women and the choline and betaine intakes of their infants. Korean J. Nutr. 43, 588-596. https://doi.org/10.4163/kjn.2010.43.6.588
  10. Kim, J. A., Kang, Y. S. and Lee, Y. S. (2003) Role of $Ca^{2+}$-activated Cl channels in the mechanism of apoptosis induced by cyclosporin A in a human hepatoma cell line. Biochemical and Biophysical Research Communications 309, 291-297. https://doi.org/10.1016/j.bbrc.2003.07.004
  11. Kim, P. N. and Jeong, C. S. (2010) Anti-gastritis and anti-oxidant effects of Chenopodium album Linne fractions and betaine. Biomolecules and Therapeutics 18, 433-441. https://doi.org/10.4062/biomolther.2010.18.4.433
  12. Kitagawa, H., Takeda, F. and Kohe, H. A. (1986) Simple method for estimation of gastric mucus and effects of antiulcerogenic agents on the decrease in mucus during water-immersion stress in rats. Arzneimittelforschung 36, 1240-1244.
  13. La Casa, C., Villegas, I., Alarcon de la Lastra, C., Motilva, V. and Martín Calero, M. J. (2000) Evidence for protective and antioxidant properties of rutin, a natural fl avone, against ethanol induced gastric lesions. J. Ethnopharmacol. 71, 45-53. https://doi.org/10.1016/S0378-8741(99)00174-9
  14. Lee, C. H., Kim, I. H., Kim, Y. E., Oh, S. W. and Lee, H. J. (2004) Determination of betaine from Saliconia herbacea L. J. Korean Soc. Food Sci. Nutr. 33, 1584-1587. https://doi.org/10.3746/jkfn.2004.33.9.1584
  15. McConkey, D. J. and Orrenius, S. (1996) The role of calcium in the regulation of apoptosis. J. Leukoc. Biol. 59, 775-783.
  16. McQuaid, K. R. and Isenberg, J. I. (1992) Medical therapy of peptic ulcer disease. Surg. Clin. North Am. 72, 285-316.
  17. Mizui, T. and Dodeuchi, M. (1983) Effect of polyamines on acidifi ed ethanol induced gastric lesion in rats. Jpn. J. Pharmacol. 33, 939-945. https://doi.org/10.1254/jjp.33.939
  18. Nishimiki, M., Rao, N. A., Appaji, N. and Yagi, K. (1972) The occurrence of superoxide anion in the reaction of reduced henazine methosulfate and molecular oxygen. Biochemical and Biophysical Research Communications. 46, 849-854. https://doi.org/10.1016/S0006-291X(72)80218-3
  19. Oh, S. K., Kim, D. J., Chun, A. R., Yoon, M. R., Kim, K. J., Lee, J. S., Hong, H. C. and Kim, Y. K. (2010) Antioxidant compounds and antioxidant activities of ethanol extracts from milling by-products of rice cultivars. J. Korean Soc. Food Sci. Nutr. 39, 624-630. https://doi.org/10.3746/jkfn.2010.39.4.624
  20. Oyaizu, M. (1986) Studied on products of browning reaction. Antioxidative activities of products of browning reaction prepared from glucosamine. Jpn. J. Nutr. 44, 307-315. https://doi.org/10.5264/eiyogakuzashi.44.307
  21. Shay, H., Komarov, S. A., Fels, S. S. and Meranze, D. (1945) A simple method for the uniform production of gastric ulceration in the rat. Gastroenterol. 4, 43-61.
  22. Sternas, L. A., Weeks, S. W. and Kwak, L. W. (1999) Mechanisms of resistance against B-cell malignancies induced by vaccination against the immunoglobulin receptor: the case for T-cell immunity. Cancer Treat Res. 99, 267-273. https://doi.org/10.1007/978-0-585-38571-6_11
  23. Waisberg, M., Black, W. D., Chan, D. Y. and Hale, B. A. (2005) The effect of pharmacologically altered gastric pH on cadmium absorption from the diet and its accumulation in murine tissues. Food and Chemical Toxicology 43, 775-782. https://doi.org/10.1016/j.fct.2005.01.018
  24. Wang, K. J., Zhang, Y. J. and Yang, C.R. (2005) Antioxidant phenolic compounds from rhizomes of Polygonum paleaceum. J. Ethnopharmacol. 96, 483-487. https://doi.org/10.1016/j.jep.2004.09.036

피인용 문헌

  1. HangAmDan-B, an Ethnomedicinal Herbal Mixture, Suppresses Inflammatory Responses by Inhibiting Syk/NF-κB and JNK/ATF-2 Pathways vol.16, pp.1, 2013, https://doi.org/10.1089/jmf.2012.2374
  2. Modulation of the pharmacokinetics, therapeutic and adverse effects of NSAIDs by Chinese herbal medicines vol.10, pp.12, 2014, https://doi.org/10.1517/17425255.2014.970167
  3. 참마와 명아주의 항산화 및 항염증 효과 vol.43, pp.4, 2011, https://doi.org/10.15230/scsk.2017.43.4.337
  4. Effects of dietary supplementation with fermented Chenopodium album L. on growth, nutrient digestibility, immunity, carcase characteristics and meat quality of broilers vol.20, pp.1, 2011, https://doi.org/10.1080/1828051x.2021.1996289