한국가금학회지 (Korean Journal of Poultry Science)

  • 제38권3호
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  • Pages.213-223
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  • 2011
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  • 1225-6625(pISSN)
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  • 2287-5387(eISSN)
한국가금학회 (The Korean Society of Poultry Science)
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매실과 홍삼 부산물이 스트레스에 대한 육계 생체 반응에 미치는 영향

Effect of Feeding Plum and Red Ginseng Marc on Vital Reaction in Broiler Stress

  • 봉미희 (농촌진흥청 국립축산과학원) ;
  • 지상윤 (농촌진흥청 국립축산과학원) ;
  • 박준철 (농촌진흥청 국립축산과학원) ;
  • 문홍길 (농촌진흥청 국립축산과학원) ;
  • 이상철 (농촌진흥청 국립축산과학원) ;
  • 이준헌 (충남대학교 농업생명과학대학 동물자원생명과학과) ;
  • 홍준기 (농촌진흥청 국립축산과학원)
  • Bong, Mi-Hee (National Institute of Animal Science, RDA) ;
  • Ji, Sang-Yoon (National Institute of Animal Science, RDA) ;
  • Park, Jun-Cheol (National Institute of Animal Science, RDA) ;
  • Moon, Hong-Kil (National Institute of Animal Science, RDA) ;
  • Lee, Sang-Cheul (National Institute of Animal Science, RDA) ;
  • Lee, Jun-Heon (Department of Animal Science and Biotechnology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Hong, Joon-Ki (National Institute of Animal Science, RDA)
  • 투고 : 2011.08.10
  • 심사 : 2011.09.09
  • 발행 : 2011.09.30
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초록

본 연구는 매실박과 홍삼박을 사료에 첨가하여 육계 체내 생리활성 효과를 구명하고자 실시하였으며, 이를 위해 고온 스트레스 자극과 LPS 염증 반응을 유도하여 스트레스에 대한 반응을 조사하였다. 이를 위해 고온 스트레스 자극에 따른 생산성 및 혈액생화학 변화를 조사하였으며, LPS로 염증 반응이 유도된 육계에서 immunoglobulin 농도 및 비장 조직내 cytokine mRNA 발현을 조사하였다. 공시계로는 1일령 Ross종 육계수컷 192수를 선별하여 고온 환경(96수)과 일반 환경(96수)으로 구분하였다. 고온 환경은 불쾌지수 86($33^{\circ}C$, 75%)의 고온 스트레스를 자극하여 5주간의 사양 시험을 실시하였으며, 일반 환경은 5주간의 사양 시험 종료 후 LPS를 주입하였다. 환경에 따라 각각 처리당 24수(3수${\times}$8반복)씩 4처리구에 총 96수를 임의 배치하여 실시하였다. 시험구 배치는 무첨가구(negative control; C), 시판 면역 증강제 첨가구(positive control: PC, 25 ppm ${\beta}$-glucan), 매실 부산물 1% 첨가구(PM: plum marc), 홍삼 부산물 3%(RGM: red gingeng marc)로 배치하였다. 고온 스트레스에 대한 혈액생화학 조성에서 매실박 첨가구는 Mg 농도를 높게 유지하여 Ca/Mg 비율을 무첨가구보다 낮게 유지하였으며, 홍삼박 첨가구는 Mg 농도가 무첨가구와 같았지만, Ca 농도를 높여 Ca/Mg 비율을 무첨가구보다 낮게 유지하였다(P<0.05). 따라서 매실박과 홍삼박 첨가구는 고온 환경에서 무첨가구보다 C a/Mg 비율을 낮게 유지하여, Ca/Mg 비율과 정의 상관관계에 있는 cholesterol 수준을 낮추는데 긍정적으로 작용할 것이라 사료된다. LPS 접종 후 IgM 농도는 매실박 첨가구가 무첨가구에 비해 유의적으로 낮은 수준을 나타내었으며(P<0.05), 홍삼박 첨가구는 무첨가구에 비해 낮은 경향을 나타냈지만 유의적인 차이는 없었다. 또한 비장 조직 내 cytokine mRNA 발현에서 홍삼박 첨가구의 경우 공통적으로 IL-1, IL-2 및 IL-6의 mRNA 발현 비율이 무첨가구에 비해 유의적으로 낮았으며, 매실박 첨가구도 IL-1, IL-2에서 무첨가구와 베타글루칸 첨가구보다 낮게 나타났다(P<0.05). 따라서 육계에서 매실박은 LPS 감염 시 IL-1, IL-2 같은 친염증 사이토카인의 발현을 감소시킴으로 염증 반응을 줄여 IgM 상승을 억제하는 것으로 사료된다. 홍삼박 첨가구의 경우 친염증성 사이토카인 억제 효과가 높았지만 항체 농도는 무첨가구와 같은 수준으로 상승하였는데, 생산성, 면역 반응을 개선할 수 있는 첨가 수준에 대한 추가적인 연구가 필요할 것으로 사료된다. 또한 이 실험에서 양성 대조군으로 활용한 ${\beta}$-glucan 전체적으로 긍정적인 효과를 보이지 않았는데, 양성 대조군 활용 시 첨가 수준에 대한 세밀한 검토가 필요할 것으로 사료된다. 결론적으로 매실박과 홍삼박은 고온 스트레스와 외부 병원성 물질 자극에 대한 반응을 최소화하는데 긍적적으로 작용하는 것으로 사료된다.

This study was conducted to test the efficacy of plum (Prunus mume) and red ginseng (Panax ginseng C.A Meyer) marc as stress inhibitors under heat stress and lipopolysaccharide (LPS) challenge in broilers by investigating their effects on blood biochemical parameters, immunoglobulin concentration and splenic cytokine mRNA expressions. A total of one hundred ninety-two 1-d-old male broiler chicks (Ross 308) were divided into 2 stress conditions (heat and LPS) experiments. Each experiment was divided into 4 treatment groups with 8 replicates of 3 birds in each group. NC (negative control, no immune substances), PC (positive control, 25 ppm ${\beta}$-glucan), PM (1% plum marc) and RGM (3% red ginseng marc) treatments were administered with respective substance through water supplementation. During heat stress, The Ca/Mg ratio in PM and RGM was significantly decreased in comparison with that of NC (P<0.05). The immunoglobulin M was significantly lower in PM than in NC (P<0.05). Expression patterns of splenic cytokine mRNAs (IL-1, IL-2 and IL-6) were similar over the treatment. Expression rates of IL-1 and IL-2 in PM were significantly decreased in comparison with NC. Also, expression rates of IL-1, IL-2 and IL-6 were significantly lower in RGM than in NC (P<0.05). In conclusion, the dietary supplementation of plum and red ginseng marc improved coping ability to heat stress by preventing Ca/Mg ratio increment and by inhibiting inflammatory response in broiler chicks. However, it is necessary to determine optimal dietary level of red ginseng marc for improving growth performances in broiler chickens. These results suggest the possibility that plum and red ginseng marc could be used as the stress inhibitor under heat stress and inflammatory response in broiler chicks.

키워드

참고문헌

  1. Altura BM, Altura Bt 1981 Magnesium ions and contraction of vascular smooth muscles: Relationship to some cardio vascular dioseases. Fed Proc 40:2672-2679.
  2. AOAC 1995 Official Method of Analysis. 16th ed. Association of Official Analytical Chemists, Washington DC USA.
  3. Bhattachary SK, Mirata SK 1991 Anxiolytic activity of Panax ginseng roots: man experimental study. J Ethnopharmacology 34:87-92. https://doi.org/10.1016/0378-8741(91)90193-H
  4. Chang EJ, Park TK, Han YN, Hwang KH 2007 Conditioning of the extraction of acidie polysaccharide from red ginseng marc. Korean J Pharmacogn 38(1):56-61.
  5. Chen HL, Li DF, Chang BY, Gong LM, Dai JG, Yi GF 2003 Effect of Chinese herbal polysaccharides on the immunity and growth performance of young broilers. Poultry Sci 82:364-370. https://doi.org/10.1093/ps/82.3.364
  6. Chomczynski P, Sacchi N 1987 Single-step method of RNA isolation by guanidium thiocyartate-phenol-chloroform extraction. Anal Biochem 162:156-159.
  7. Corica F, Allegra A, Di Benedetto A, Giacobbe MS, Romano G, Cucinotta D, Buemi M, Ceruso D 1994 Effects of oral magnesium supplementation on plasma lipid concentrations in patients with non insulin dependent diabetes mellitus. Magnesium Res 7(1):43-47.
  8. Diaz GJ, Roldan LP, Cortes A 2003 Intoxication of Crotalaria pallid seeds to growing broiler chicks. Vet Hum Toxicol 45:187-189.
  9. Duncan DB 1955 Multiple range and multiple F test. Biometrics 11:1-42. https://doi.org/10.2307/3001478
  10. Givalois L, Dormand J, Mekaouche M, Solier MD, Bristow AF, Ixart G, Siaud P, Assenmacher I, Barbanel G 1994 Temporal cascade of plasma level surges in ACTH, corticosterone, and cytokines in endotoxin-challenged rats. Am J Physiol 267(R):164-170.
  11. Gu YH, Takage Y, Nakamura T, Hasegawa T, Suzuki I, Oshima M, Tawaraya H, Niwano Y 2005 Enhancement of radioprotection and anti-tumor immunity by yeast-derived betaglucan in mice. J Med Food 8:154-158. https://doi.org/10.1089/jmf.2005.8.154
  12. Gums JG 1987 Clinical significance of magnesium : A review. Drug Intell Clin Pharm 7(21):240-246.
  13. Hernandez F, Madrid J, Garcia V, Orengo J, Megias MD 2004 Influence of two plant extracts on broilers performance, digestibility, and organ size. Poultry Sci 83:169-174. https://doi.org/10.1093/ps/83.2.169
  14. Ikeda I 2008 Multifunctional effects of green tea catechins on prevention of the metabolic syndrome. Asia Pac J Clin Nutr 17:273-274.
  15. Iseri LT, Frency JH 1984 Magnesium, nature's physiologic calcium blocker. Am Heart J 108:188-193. https://doi.org/10.1016/0002-8703(84)90572-6
  16. Iseri LT, Frency JH 1984 Magnesium, nature's physiologic calcium blocker. Am Heart J 108:188-193. https://doi.org/10.1016/0002-8703(84)90572-6
  17. Kim KJ, Bae JH 1999 Effects of sports drink including the extract from Prunus mume on the changes of respirotory variables, heart rate, and blood lactate concentration in submaximal exercise. J East Asian Dietary Life 9:177-187.
  18. Klasing KC 1998 Avian macrophage: Regulators of local and systemic immune response. Poultry Sci 77:983-989. https://doi.org/10.1093/ps/77.7.983
  19. Ko YH, Yang HY, Kang SY, Kim ES, Jang IS 2007 Effects of a blend of Prunus mume extract as an alternative to antibiotics on growth performance, activity of digestive enzymes and microflora population in broiler chickens. J Anim Sci & Technol (Kor) 49(5):611-620. https://doi.org/10.5187/JAST.2007.49.5.611
  20. Koenen ME, Boonstra-Blom AG, Jeurissen SHM 2002 Immunological differences between layer- and broiler-type chickens. Vet Immunol Immunopathol 89:47-56. https://doi.org/10.1016/S0165-2427(02)00169-1
  21. Kwak YS, Park JD, Yang JW 2003 Present and its prospect of red ginseng efficacy research. Food Industry & Nutr 8(2):30-37.
  22. Leshchinsky TV, Klasing KC 2003 Profile of chicken cytokines induced by lipopolysaccharide is modulated by dietary $\alpha$-tocopheryl acetate. Poultry Sci 82:1266-1273. https://doi.org/10.1093/ps/82.8.1266
  23. Lipsett PA 2006 Surgical cricical care: Fungal infections in surgical patients. Crit Care Med 34:S215-224. https://doi.org/10.1097/01.CCM.0000231883.93001.E0
  24. Lumeiji JT 1997 Avian clinical biochemistry. In: Clinical Biochemistry of Domestic Animals. Keneko JJ, Harvey JW, Bruss ML (eds) Academic Press. pp, 857-883.
  25. Mantovani MS, Bellini MF, Angeli JP, Oliverira RJ, Silva AF, Ribeiro LR 2008 $\beta$-Glucan in promoting health: Prevention against mutaion and cancer. Mutat Res 658(3):154-161. https://doi.org/10.1016/j.mrrev.2007.07.002
  26. Muramatsu K, Fukuyo M, Hara Y 1986 Effect of green tea catechins on plasma cholesterol level in cholesterol fed rats. J Nutr Sci Vitaminol 32:613-622. https://doi.org/10.3177/jnsv.32.613
  27. Nakajima S, Fujita K, Inoue Y, Nishio M, Seto Y 2006 Effect of the folk remedy, Bainiku-ekisu, a concentrate of Prunus mume juice, on Helicobacter pylori infection in humans. Helicobacter 11(6):589-591. https://doi.org/10.1111/j.1523-5378.2006.00463.x
  28. Ottaway CA, Fong IW, da Silva B, Singer W, Karrass L 1998 Integrative aspects of a human model of endotoxemia. Can J Physiol Pharamacol 76:473-478. https://doi.org/10.1139/y98-041
  29. Park LY, Chae MH, Lee SH 2007 Antibacterial activity of fresh Prunus mume and Prunus mume liqueur byproduct. J Fd Hyg Safely 22(2):77-81.
  30. Queenan KM, Stewart ML, Smith KN, Thomas W, Fulcher RG, Slavin JL 2007 Concentrated oat $\beta$-glucan, a fermentable fiber, lowers serum cholesterol in hypercholesterolemic adults in a randomized controlled trial. Nutr J 6:1-8. https://doi.org/10.1186/1475-2891-6-1
  31. Ryu GH 2003 Present status of ginseng products and its manufacturing process. Food Industry & Nutr 8(2):38-42.
  32. Szenberg A, Lind P, Clarke K 1965 IgG and IgM antibodies in fowl serum. Aust Exp Biol Med Sck 43:451-454. https://doi.org/10.1038/icb.1965.79
  33. Swaminathan S, Mrudula Wilfred, Priya Gangadaran, Mitra Ghosh 2011 Association between lipid profile and macro metals calcium and magnesium. Journal of Experimental Sciences 2(6):7-17.
  34. SAS, 2008 SAS User's Guide, Statistical. Analysis System Inst. Inc. Cary. NC.
  35. Sen C, Packer L 1996 Antioxidant and redox regulation of gene transcription. FASEB J 10:709-720. https://doi.org/10.1096/fasebj.10.7.8635688
  36. Sheo HJ, Lee MY, Chung DL 1990 Effect of Prunus mume extract on gastric secretion in rats and carbon tetrachloride induced liver damage of rabbits. Korean Soc Food Nutr 19:21-26.
  37. Sibbald IR 1976 A bioassay for true metabolizable energy in feedingstuffs. Poultry Sci 48:41-43.
  38. Suzuki M, Takatsuki F, Maeda Y, Hamuro J, Chihara G 1994 Antitumor and immunological activity of lentinan in comparison with LPS. Int J Immunopharmacol 16:463-468. https://doi.org/10.1016/0192-0561(94)90037-X
  39. Szenberg A, Lind P, Clarke K 1965 IgG and IgM antibodies in fowl serum. Aust Exp Biol Med Sci 43:451-454. https://doi.org/10.1038/icb.1965.79
  40. Van de Broek, Ir G 2000 Organic acid: Natural link between drug growth promoter. Feed Mix. Special. 9-11.
  41. 식약청 2010 생산액 집계결과.
  42. 임재웅 장인석 고영현 강선영 문양수 1999 매실의 생리활성. 한국식품영양학회지 29:19-37.
  43. 한국사양표준 2007 농촌진흥청 축산과학원.

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