Food Science of Animal Resources (한국축산식품학회지)

Korean Society for Food Science of Animal Resources (한국축산식품학회)
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A Challenging Study to Identify Target Proteins by a Proteomics Approach and Their Validation by Raising Polyclonal Antibody

  • Jeong, Da-Woon (Department of Animal Science and Institute of Rare Earth for Biological Application, Chonbuk National University) ;
  • Park, Beom-Young (Quality Control and Utilization of Animal Products Division, National Institute of Animal Science, RDA) ;
  • Kim, Jin-Hyoung (Quality Control and Utilization of Animal Products Division, National Institute of Animal Science, RDA) ;
  • Hwang, In-Ho (Department of Animal Science and Institute of Rare Earth for Biological Application, Chonbuk National University)
  • Received : 2011.04.29
  • Accepted : 2011.07.11
  • Published : 2011.08.31
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Abstract

This study was conducted to validate the theoretical feasibility of a technique to identify biomarkers in Korean native black pig (KNP) and a commercial Landrace breed. Using two-dimensional electrophoresis, we found six proteins (NADH dehydrogenase Fe-S protein 1, an unnamed protein product, similar to T-complex protein 1, annexin V = CaBP33 isoform, fatty acid-binding protein, and catechol O-methyltransferase), which appeared in KNP alone. We raised polyclonal antibodies (used as the primary antibody) for Western blotting to confirm the characteristics of the six KNP proteins. As a result, catechol O-methyltransferase, annexin V = CaBP33 isoform, and the unnamed protein product presented thicker bands in KNP than those in Landrace. Moreover, catechol O-methyltransferase was shown to be more feasible as a biomarker for KNP. However, cross-reactivity was observed with the polyclonal antibodies for KNP and the other three proteins (NADH dehydrogenase, a protein similar to T-complex protein 1, and fatty acid-binding protein). This study only showed limited results from a limited number of animals; however, our research suggests possibilities for future studies.

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References

  1. Annerbrink, K., Westberg, L. Nilwwon, S., Rosmond, R, Holm, G., and Eriksson, E. (2008) Catechol O-methyltransferase val158-met polymorphism is associated with abdominal obesity and blood pressure in men. Metabolism 7, 708-711.
  2. Bendixen, E. (2005) The use of proteomics in meat science. Meat sci. 71, 138-149. https://doi.org/10.1016/j.meatsci.2005.03.013
  3. Bowker, B. C., Botrel, C., Swartz, D. R., Grant, A. L., and Gerrard, D. E. (2004) Influence of myosin heavy chain isoform expression and postmortem metabolism on the ATPase activity of muscle fibers. Meat Sci. 68, 587-594. https://doi.org/10.1016/j.meatsci.2004.05.010
  4. Gariepy, C., Farmer, C., Lamontagne, N., Manzalini, L., and Faucitano, L. (2001) A comparative study on muscle fibers and meat quality from Large White and Upton-Meishan pigs. Proceed. 47th Int. Cong. Meat Sci. Technol., Krakow, Poland, pp. 262-263.
  5. Gerbens, F., van Erp, A. J., Harders, F. L., Verburg, F. J., Meuwissen, T. H., Veerkamp, J. H., and te Pas, M. F. (1999) Effect of genetic variats of the heart fatty acid-binding protein gene on interamuscular fat and performance traits in pig. J. Anim. Sci. 77, 846-852.
  6. Giammbanco, I., Sorci. G., Antonioli, S., Rambotti, M. G., Spreca, A., Bocchini, V., and Donato, R. (1993) Immunocy-tochemmical analysis of annexin V(CaBP33) in a human-derived giloma cell line. FEBS 323, 45-50. https://doi.org/10.1016/0014-5793(93)81445-6
  7. Goo, Y. A. and Goodlett, D. R. (2010) Advances in proteomics prostate cancer biomarker discovery. Proteomics 73, 1839-1850. https://doi.org/10.1016/j.jprot.2010.04.002
  8. Grigorieff, N. (1999) Structure of the respiratory NADH: ubiquinone oxidoreductase (complex I). Curr. Opin. Struct. Biol. 9, 476-484. https://doi.org/10.1016/S0959-440X(99)80067-0
  9. Hocquette, J. F., Isabell, C. M., Carine B. C., and Brigitte P. (2009) Functional genomics and now markers for beef production- minireview. Anim. Sci. Pap. Rep. 27, 273-280.
  10. Hwang, I. H., Park, B.Y., Cho, S. H., Kim, J.H., and Lee, J. M. (2004) Identification of muscle proteins related to objective meat quality in Korean native black pig. Asian Austral. J. Anim.17, 1599-1607. https://doi.org/10.5713/ajas.2004.1599
  11. Hwang, I. H., B. Y. Park, J. H. Kim, S. H. Cho and J. M. Lee. (2005) Assessment of postmortem proteolysis by gel-based proteome analysis and its relation to meat quality traits in pig longissimus. Meat Sci. 69, 79-91. https://doi.org/10.1016/j.meatsci.2004.06.019
  12. Jia, X. H., Hildrum, K. I., Westad, F., Kummen, E., Aass, L., and Hollung K. (2006) Change in enzyme associated with energy metablism during the early post mortem period in longissimus thoracis bovine muscle analyzed by proteomics. J. Proteome Res. 5, 1763-1769. https://doi.org/10.1021/pr060119s
  13. Jang, K.Y. and Kim, H. (2009) Purchasing situations of beef and the level of consumer's satisfaction on Australian beef. Korean Soci. Intl. Agri. 21, 85-90.
  14. Jia, X. H., Ekman, M., Grove, H., Faergestad, E. M., Aass, L., Hildrum, K. I. and Hollung, K. (2007) Proteomics change in bovine longissimus thoracis muscle during the early postmortem storage period. J. Proteome Res. 6, 2720-2731. https://doi.org/10.1021/pr070173o
  15. Kim, N. K., Lim, J. H., Song, M. J., Kim, O. H., Park. B. Y., Kim, M. J., Hwang, I. H., and Lee, C. S. (2008a) Comparisons of longissimus muscle metabolic enzymes and muscle fiber types in Korean and western pig breeds. Meat Sci. 78, 455-460. https://doi.org/10.1016/j.meatsci.2007.07.014
  16. Kim, N. K., Cho, S. H., Lee, S. H., Park, H. R., Lee, C. S., Cho, Y. M. Choy, Y. H., Yoon, D. Im, S. K., and Park, E. W. (2008b) Protein in longissimus muscle of Korean native cattle and their relationship to meat quality. Meat Sci. 80, 1068-1073. https://doi.org/10.1016/j.meatsci.2008.04.027
  17. Kubota, H., Hynes, G., and Willison, K. (1995) The chaperonin containing t-complex polypeptide 1 (TCP-1). Multisubunit machinery assisting in protein folding and assembly in the eukaryotic cytosol. Eur. J. Biochem. 230, 3-16. https://doi.org/10.1111/j.1432-1033.1995.tb20527.x
  18. Lametsch, R. and Bendixen, E. (2001) Proteome analysis applied to meat science: Characterizing postmortem changes in porcine muscle. J. Agr. Food Chem. 49, 4531-4537. https://doi.org/10.1021/jf010103g
  19. Lee, K. Y. and Kim, H. (2009) Purchasing situation of beef and the level of consumer's satisfaction on Australian beef. Korean J. Intl. Agri. 21, 85-90.
  20. Lee, S. M. and Jin, Y. H. (2009a) Research on consumer awareness due to origin declaration of beef in general restaurant. Korean J. Cul. Res. 15, 112-127.
  21. Lee, S. M., Lee, W. W., Lee, G. R., and Lee, D. S. (2009b) Differentiation of Hanwoo and other breeds of cattle using PCR-PFLP and Allele-specific PCR. Ann. Rep. Busan Metro. City Inst. Health Environ. 19, 88-93.
  22. Lim, D., Cho, Y. M., Lee, S. H., Sung, S., Nan, J., Yoon, D., Shin, Y., Park, H., and Kim, H. (2010) An integrated genomic resource based on Korean Cattle (Hanwoo) transcripts. Asian-Aust. J. Anim. Sci. 23, 1399-1404. https://doi.org/10.5713/ajas.2010.90250
  23. Martin, I., Garcia, T., Fajardo, V., pez-Calleja, I. L., Rojas, M., Hemandez, P. E., Gonza, I., and Martin, R. (2007) Mitochondrial markers for the detection of four duck species and the specific identification of Muscovy duck in meat mixtures using the polymerase chain reaction. Meat Sci. 76, 721-729. https://doi.org/10.1016/j.meatsci.2007.02.013
  24. Mastellone, V., Massimini, G., Pero, M. E., Cortese, L., Piantedosi, D., Lombardi, P., Britti, D., and Avallone, L. (2011) Effect of passive transfer status on growth performance in Buffalo calves. Asian-Aust. J. Anim. Sci. 24, 952-956. https://doi.org/10.5713/ajas.2011.10348
  25. Mullen, A. A. M., Stapleton, P. C., Corcoran, D., Hamill, R. M., and White, A. (2006) Understanding meat quality through the application of genomic and proteomics approaches. Meat Sci. 74, 3-16. https://doi.org/10.1016/j.meatsci.2006.04.015
  26. Niehaus, D. J. H., Kinnear, C. J., Corfield, V. A., Toit, P. L., Kradenburg, J. Moolman-Smook, J. C., Weyer, J. B., Potgieter, A., Seedat, S. Emsley, R. A., Knowles, J. A., Brink, P. A., and Stein, D. J. (2001) Association between a catechol-omethyltransferase polymorphism and obsessive-compulsive disorder in the Afrikaner population. J. Affect. Disorders 65, 61-65. https://doi.org/10.1016/S0165-0327(00)00246-9
  27. Oliveira, G. C., Moreira, I., Souza, A. L. P., Murakami, A. E., Parra, A. R. P., Carvalho, P. L. O., and Borile, M. D. (2011) Corns with different nutritional profiles on growing and finishing pig feeding (30 to 90 kg). Asian-Aust. J. Anim. Sci. 24, 982-992. https://doi.org/10.5713/ajas.2011.90587
  28. Park, B. Y., Kim, N. K., Lee, C. S., and Hwang, I. H. (2007) Effect of fiber type on postmortem proteolysis in longissimus muscle of Landrace and Korean native black pigs. Meat Sci. 77, 482-491. https://doi.org/10.1016/j.meatsci.2007.04.022
  29. Park, J. K., Lee, J. Y., Chea, B. H. J., and Ohh, S. J. (2009) Effect of different sources of dietary chromium on growth, blood profiles and carcass traits in growing-finishing pig. Asian-Aust. J. Anim. Sci. 22, 1547-1554. https://doi.org/10.5713/ajas.2009.80633
  30. Pennington, S. R. and Dunn, M. J. (2001) Proteomics from protein sequence to function. Springer-Verlag, New York, USA.
  31. Shi, M., MCaudle, M. W., and Zhang, J. (2009) Biomarker discovery in neurodegenerative diseases: A proteomic approach. Neurobiol. Dis. 35, 157-164. https://doi.org/10.1016/j.nbd.2008.09.004
  32. Waritthitham, A., Lambertz, C., Langholz, H. J., Wicke, M., and Gauly, M. (2010) Muscle fiber characteristics and their relationship to water holding capacity of Longissimus dorsi muscle in Brahman and Charolais crossbred bulls. Asian-Aust. J. Anim. Sci. 23, 665-671. https://doi.org/10.5713/ajas.2010.90482
  33. Westermeier, R. and Naven, T. (2002) Proteomics in practical-A laboratory manual of proteomics analysis. Wiley-Vch, Freibury, Germany.
  34. Yan, J. X., Harry, R. A., Wait, R., Welson, S. Y., Emery, P. W., Preedy, V. R., and Dunn, M. J. (2001) Separation and identification of rate skeletal muscle proteins using two-dimensional gel electrophoresis and mass spectrometry. Proteomics 1, 424-434. https://doi.org/10.1002/1615-9861(200103)1:3<424::AID-PROT424>3.0.CO;2-Y
  35. Yeo, J. S., Lee, J. S., Lee, C. H., Jung, Y. J., and Nam, D. H. (2000) Identification of genetic markers for Korean native cattle (Hanwoo) by RAPD analysis. Biotechnol. Bioprocess Eng. 5, 23-26. https://doi.org/10.1007/BF02932348
  36. Yokota, S. I., Yanagi, H. Yura, T., and Kubota, H. (2000) Upregulation of cytosolic chaperonin CCT subunits during recovery from chemical stress that causes accumulation of unfolded proteins. Eur. J. Biochem. 267, 1658-1664. https://doi.org/10.1046/j.1432-1327.2000.01157.x