Journal of Sasang Constitutional Medicine (사상체질의학회지)

The Society Of Sasang Constitutional Medicine (사상체질의학회)
권호 표지
DOI QR코드

DOI QR Code

The Effect of Suhwagije-tang Distillate on the Immune Activity of Spleen Cells of Aged Rats

수화기제탕(水火旣濟湯) 증류액이 노화 흰쥐의 비장세포 면역활성에 미치는 영향

  • Choi, Jae-Song (Dept. of Sasang Constitutional Medicine, College of Oriental Medicine, Daejeon Univ.) ;
  • Bae, Na-Young (Dept. of Sasang Constitutional Medicine, College of Oriental Medicine, Daejeon Univ.) ;
  • Ahn, Teak-Won (Dept. of Sasang Constitutional Medicine, College of Oriental Medicine, Daejeon Univ.)
  • 최재송 (대전대학교 한의과대학 사상체질의학과) ;
  • 배나영 (대전대학교 한의과대학 사상체질의학과) ;
  • 안택원 (대전대학교 한의과대학 사상체질의학과)
  • Received : 2011.07.29
  • Accepted : 2011.08.20
  • Published : 2011.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

1. Objectives: The purpose of this study is to investigate the effect of Suhwagije-tang(SGT) distillate on the immune activity of spleen cells of aged SD rats. 2. Methods: We used 10, 50, 72 weeks old SD rats in this study. Spleen cells from SD rats were stimulated with ConA and treated with 1% Vitamin C(Vit.C) or Suhwagijetang distillate(SGT). After 24 hours, the concentrations of IL-2, IL-4, IL-10, IFN-${\gamma}$ in the cell culture supernatant were measured by ELISA. 3. Results and Conclusions 1) At all concentration of SGT distillate, survival rates of liver cells were higher than the control group. In addition, 50% SGT distillate group's cell survival rates were significantly higher than other groups. 2) In 10 weeks SD rats(SGT group), the concentration of IL-2 significantly decreased in comparison with ConA group, Vit.C group. In 52 weeks SD rats(SGT group), the concentration of IL-2 significantly decreased in comparison with ConA group. 3) In 10, 52 weeks SD rats(SGT group), the concentration of IL-4 significantly decreased in comparison with ConA group. 4) In 10 weeks SD rats(SGT group), the concentration of IL-10 significantly decreased in comparison with ConA group. And in 72 weeks SD rats(SGT group), the concentration of IL-10 significantly increased in comparison with Vit.C group. 5) In 52, 72 weeks SD rats(SGT group), the concentration of IFN-${\gamma}$ significantly decreased in comparison with 10 weeks SD rats(SGT group). These results suggest that Suhwagije-tang(SGT) distillate has the effect of increasing the immune activity of spleen cells of aged SD rats.

Keywords

References

  1. 통계청. 2010 고령자통계. 통계소식. 2010. Available from:URL:http://kostat.go.kr.
  2. 손장락. 활성산소와 항산화제. 서울:바이오메디컬. 2004:130.
  3. 장휘숙. 성인심리학-성인발달, 노화, 죽음. 서울:박영사. 2006:78.
  4. 전국한의과대학 사상의학교실. 개정증보사상의학. 서울:집문당. 2005:81-82, 200-201,312,390-394,410, 694.
  5. 杜鎬京. 東醫腎系學. 서울:東洋醫學硏究院. 1991:1093-1100,1325-1383.
  6. 이경우. 편주역해 황제내경소문 1권. 서울:여강출판사. 2000:31,201-202.
  7. 김숙희, 김화영. 노화. 서울:믿음사. 1995:77-88.
  8. 구병삼. 노화방지의학. 서울:칼빈서적. 2003:37-44.
  9. 강태숙 외 19명. 임상면역학. 서울:청구문화사. 2005:11-23.
  10. Bae JK, Ahn TW. Antioxidative activity of Suhwagijetang in aged rats. in press (Korean).
  11. Lee JH, Goo DM, Ahn TW. Effects of Suhwagije-tang distillate on serum and testosterone in aging rats. Journal of Sasang Constitutional Medicine. 2010;22(4): 85-97. (Korean)
  12. 보건복지부. 우리나라의 보건의료 실태 분석 결과. Available from: http://news.chosun.com/site/data/html _dir/
  13. Cheon JS. Concept of ageing. J Korean Soc Biol Ther Psychiatry. 2007;13(2):129-137. (Korean)
  14. 이정찬. 신 사상의학론. 2권. 서울:목과토. 2003: 191,205,207,228,229,238,239.
  15. 대한미생물학회. Kuby 면역학. 서울:이퍼블릭. 2008:43-54.
  16. Lim KH, Kim SY, Kim HM, Kim JS, Jo CJ, Yu YC, An HJ. Effect of Gypsum Fibrosum on Interleukin-4 Secretion of Mice Splenocytes. Journal of the Korea Association of Herbology. 2002;17(2):139-144. (Korean)
  17. Shim SM, Joo SG, Kim GW, Koo BS. Effects of Baegumhwan administration on immune-function in ICR mice stressed by electric footshock. Journal of Oriental Neuropsychiatry. 2002;13(1):1-15. (Korean)
  18. Choi BC, Ahn TW. Anti-Oxidant Effect of Hyangsayangyi- tang Decoction in Stomach, Spleen and Pancreas Cell of SD Rats. J Sasang Constitut Med. 2008;20(2): 72-84. (Korean)
  19. Lee HE, Ahn TW. Anti-Oxidant Effect of Hyeongbangjiwhang- tang Decoction in Kidney, Bladder and Spleen Cell of SD Rats. J Sasang Constitut Med. 2008;20(2): 85-97. (Korean)
  20. 나종득, 안희경. Concanavalin A의 자극에 의한 생쥐 오금림프절내 림프소절과 속겉질의 변화에 관한 형태계측학적 연구. 경희대학교 대학원 박사학위 논문. 2000:1-37.
  21. Yoon HG, Bang DW, Park SH, Ahn JH, Seo J, Yoon YJ, Hyon MS, Kim SK, Kwon YJ. The change of serum level of total antioxidant status and cytokine, and in-stent restenosis after supplementation of antioxidant. The Korean Journal of Internal Medicine. 2006;71(2): 158-165. (Korean)
  22. Kim YH, Kim JW, Kim CH, Cho MK, Kim SM, Kang WD, Cho HY, Yang SY, Ahn BW, Song TB. Effect of vitamin C and E on the Lipid peroxide, antioxidant ability, and antioxidant vitamin Levels in maternal and umbilical venous plasma. Korean Journal of Obstetrics & Gynecology. 2009;52(11):1117-1126. (Korean)
  23. Kwak YS. Studies of Exercise-Induced Allergy Anaphylaxis Mechanisms and the Effects of Vitamin C and Catalase Supplementation in Exercise-Induced Allergy Anaphylaxis Models. Journal of life science. 2010;20(4):511-518. (Korean) https://doi.org/10.5352/JLS.2010.20.4.511
  24. Lee SY, Ahn TW. Anti-aging Effect of Tae-Eumin's Nocyongdaebo-tang(NYD) in Aged Rats. J Sasang Constitut Med. 2008;20(2):58-71. (Korean)
  25. Sun TC, Ahn TW. Anti-aging Effect of Sipyimigwanjungtang in Aged Rats. J Sasang Constitut Med. 2008;20(2): 98-110. (Korean)
  26. Lee HS, Ahn TW. Anti-aging and Anti-oxidative Effect of Gongjinhugwon-dan in Early Stages of Aging Rats. J Sasang Constitut Med. 2007;19(3): 242-256. (Korean)
  27. Lim JP, Ahn TW. The Anti-oxidative and Immune-regulatory Effect of Chungsimyeonja-tang in Aged Rat. Journal of Sasang Constitutional Medicine. 2007;19(3): 227-241. (Korean)
  28. 장응찬. 흰쥐에서 연령이 골격근의 당대사에 미치는 영향. 영남의대학술지. 2001;18(1):94-100.
  29. Park SK, Lee HJ, Kim HT, Whang WW. An experimental study of oriental medicine on cure for dementia. Journal of Oriental Neuropsychiatry. 1998; 9(2):19-35. (Korean)
  30. Murphy MP, Rick J Th, Milgram NW, Ivy GO. A simple and rapid test of sensorimotor function in the aged rat. Neurobiology of Learning and Memory. 1995; 64:181-186. https://doi.org/10.1006/nlme.1995.1057
  31. 이귀녕, 권오헌. 임상병리 파일. 제3판. 서울:의학 문화사. 2003:716-732.
  32. 표석능, 손은화. 면역학개론. 서울:신일북스. 2008: 145-158.
  33. Mire-Sluis,A.R.,R.Thorpe. CYTOKINES. San Diego, California:Academic Press. 1998:19-400.
  34. 서설. 임상면역학 제2판. 서울:고려의학. 2009:121, 126-131.
  35. Chang Joon Bae, Min Sun Kim, Su Youn Yim, Dae Youn Hwang, Cheul Kyu Kim, Byoung Guk Kim, Sun Bo Shim, Seung Wan Jee, Su Hae Lee, Ji Soon Sin, Byoung Chun Lee, Jong Min Woo, Mee Kyung Jang, Kab Ryong Chae. Phthalic Anhydride Can Induce the Difference on Skin Inflammation and Interleukin-4 Expression in BALB/c, TG2576 and NOD Mice. Laboratory Animal Research. 2008;24(1):27-31.
  36. 식품의약품안정청 바이오생약국. 생약․한약제제의 효력시험 가이드라인(아토피피부염). 서울:바이오생약국 생약제제과. 2010:7.
  37. 강혜영, 김동수, 이영진, 김규언, 정병주, 이기영. 소아 천식 환아에서 혈청 Interleukin - 10치의 변동에 관한 연구. 대한 천식 및 알레르기학회 추계학술대회 초록집. 1995:485.