Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Anti-cancer Activity of Human Colon Cancer (HT-29) Cell Line from Different Fraction of Zanthoxylum schnifolium Fruits

산초 (Zanthoxylum schnifolium)열매 분획물의 대장암세포(HT-29)에 대한 항암 효과

  • Han, Woong (Department of Medical Biotechnology, College of Biomedical Science, Kangwon National University) ;
  • Hu, Wei-Cheng (Department of Medical Biotechnology, College of Biomedical Science, Kangwon National University) ;
  • Lee, Young-Mee (Department of Nursing, Kangwon National University, Samcheok 2nd Campus)
  • 한웅 (강원대학교(춘천캠퍼스) 의생명과학대학 의생명공학과) ;
  • 호위성 (강원대학교(춘천캠퍼스) 의생명과학대학 의생명공학과) ;
  • 이영미 (강원대학교(삼척캠퍼스) 한방보건복지대학 간호학과)
  • Received : 2011.08.09
  • Accepted : 2011.09.23
  • Published : 2011.09.30
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Abstract

This study was presented to the anti-cancer activity from different fraction of Zanthoxylum schnifolium fruits. The values for human colon cancer cell(HT-29) survival rate of 0.3 mg/mL of 70% EtOH and 70% MeOH ethyl acetate fraction extracts were 7.62${\pm}$0.173%, 7.66${\pm}$0.037%, respectively. It was shown that human colon cancer cell(HT-29) survival rate was in a dose-dependent manner. The percentages of cells were increased in the sub-G0 and G0/G1 phase region, meaning that cell proliferation was decreased. The RT-PCR demonstrated that 70% EtOH and 70% MeOH ethyl acetate fraction extracts were down-regulated the expression of Bcl-2 and survivin genes in HT-29 cells. We examined that 70% EtOH and 70% MeOH ethyl acetate fraction extracts anti-cancer activities initiated through ROS generation suggesting that HT-29 cells treated with ethyl acetate fraction extracts induced ROS generation. Our results revealed that the Zanthoxylum schnifolium fruit may expect for anti-cancer activities in HT-29 cells.

Keywords

References

  1. 박춘근 (2002) 현대인을 위한 민간약초, 작물시험장.
  2. Lee, M. S. and Chung, M. S. (2000) Analysis of volatile flavor components from Zanthoxylum schinifolium and sensory evaluation as natural spice. Korean J. Soc. Food Sci. 16: 216- 220.
  3. Jang, M. J., Woo, M. H., Kim, Y. H., Jun, D. Y. and Rhee, S. J. (2005) Effects of antioxidative, DPPH radical scavenging activity and antithrombogenic by the extract of Sancho(Zanthoxylum schinifolium). Korea J. Nutrition. 38: 386-394.
  4. Kim, J. H. (2003) Variation of the leaf monoterpenes concentration of Zanthoxylum schinifolium at Mt. Muhak. J. Basic Sci. 18: 137-149.
  5. Lim, S. J., Han, H. K. and Ko, J. H. (2003) Effects of edible and medicinal plants intake on blood glucose, glycogen and protein levels in streptozotocin induced diabetic rats. Korean J. Nutrition. 36: 981-989.
  6. Kim, J. S., Jun, D. Y., Woo, M. H., Rhee, I. K. and Kim, Y. H. (2006) Chemical composition and antitumor apoptogenic activity of methylene chloride extracts from the leaves of Zanthoxylum schinifolium. J. life sci. 16: 546-554. https://doi.org/10.5352/JLS.2006.16.3.546
  7. Jang, M., Lee, J. H., Seo, J. and Kim, G. H. (2010) Antibacterial Activities of Essential Oil from Zanthoxylum schinifolium Against Food-Borne Pathogens. Korean J. Food Cookery Sci. 26: 206-213.
  8. Jang, H. S., Rhee, S. J. Woo, M. H. and Cho, S. H. (2007) Anti-thrombogenic and anti-inflammention effect of solvent fraction from leaves of Zanthoxylum schinifolium (Sancho Namu) in rat fed high fat diet. Korean J. Nutrition 40: 606-615.
  9. Chen, I. S., Lin, Y. Tsai, I. L., Teng, C. M., Ko, F. N., Ishikawa, T. and Ishii, H. (1995) Coumarins and anti-platelet aggregation constituents from Zanthoxylum schinifolium. Phytochemistry 39: 1091-1097. https://doi.org/10.1016/0031-9422(95)00054-B
  10. Kim, S. I. and Han, Y. S. (1997) Isolation and identification of antimicrobial compound from sancho (Zanthoxylum schinifolium). Korean J. Food Sci. Technol. 13: 56-63.
  11. Kim, J. S., Koo, K. M. and Jung, Y. H. (2004) Antimicrobial activities of Zanthoxylum schinifolium extract against Vibrio parahaemolyticus. Korean J. Food Sci. Nutr. 33: 500-504. https://doi.org/10.3746/jkfn.2004.33.3.500
  12. Yoon, D. H. and Choi, Y. S. (2008) Influence of red pepper (Capsicum annuum L.) seed oil and sancho (Zanthoxylum schinifolium) seed oil on serum and liver lipids profiles in rats. Korean J. Food Sci. Technol. 40: 96-100.
  13. Oh S. M., Han, W. and Wang, M. H. (2010) Antioxidant and $\alpha$-glucosidase Inhibition Activity from Different Extracts of Zanthoxylum schnifolium Fruits. Kor. J. Pharmacogn. 41 : 130-135.
  14. Han W. and Wang, M. H. (2010) Radical Scavenging and Anti-inflammation Activities from Different Extracts of Zanthoxylum schnifolium Fruits. Kor. J. Pharmacogn. 41: 250- 254.
  15. Sauer, H., Wartenberg, M. and Hescheler, J. (2001) Reactive oxygen species as intracellular messengers during cell growth and differentiation. Cell. Physiol. Biochem. 11: 173-186. https://doi.org/10.1159/000047804
  16. Kuo, P. L., Chen, C. Y. and Hsu, Y. L. (2007) Isoobtusilactone a induces cell cycle arrest and apoptosis through reactive oxygen species/apoptosis signal-regulating kinase 1 signaling pathway in human breast cancer cells. Cancer Res. 67: 7406- 7420. https://doi.org/10.1158/0008-5472.CAN-07-1089
  17. Korsmeyer, S. J. (1995) Regulators of cell death. Trends Genet. 11: 101-105. https://doi.org/10.1016/S0168-9525(00)89010-1
  18. Debatin, K. M. (2004) Apoptosis pathways in cancer and cancer therapy. Cancer Immunol. Immunother. 53: 153-159. https://doi.org/10.1007/s00262-003-0474-8
  19. Paoluzzi, L. and O'Connor, O. A. (2010) Targeting survival pathways in lymphoma. Adv. Exp. Med. Biol. 687: 79-96.
  20. Desai, A., Vyas, T. and Amiji, M. (2008) Cytotoxicity and apoptosis enhancement in brain tumor cells upon coadministration of paclitaxel and ceramide in nanoemulsion formulations. J. Pharm. Sci. 97: 2745-275. https://doi.org/10.1002/jps.21182
  21. Yin, Y., Heo, S. I., Roh, K. S. and Wang, M. H. (2009) Biological activities of fractions from methanolic extract of Picrasma quassioides. J. Plant Biol. 52: 325-331. https://doi.org/10.1007/s12374-009-9042-x
  22. Wang, L., Xu, M. L., Hu, J. H., Rasmussen, S. K. and Wang, M. H. (2011) Codonopsis lanceolata extract induces G0/G1 arrest and apoptosis in human colon tumor HT-29 cells - Involvement of ROS generation and polyamine depletion. Food and Chemical Toxicology 49: 149-154. https://doi.org/10.1016/j.fct.2010.10.010
  23. Ling, Y. H., Liebes, L., Zou, Y. Y. and Perez-Soler, R. (2003) Reactive oxygen species generation and mitochondrial dysfunction in the apoptotic response to Bortezomib, a novel proteasome inhibitor, in human H460 non-small cell lung cancer cells. J. Biol. Chem. 278: 33714-33723. https://doi.org/10.1074/jbc.M302559200
  24. Zhang, Y. D. and Chen, F. (2004) Reactive oxygen species (ROS), troublemakers between nuclear factor-jB (NF-jB) and c-Jun NH2-terminal kinase (JNK). Cancer Res. 64: 1902- 1905. https://doi.org/10.1158/0008-5472.CAN-03-3361
  25. Moungjaroen, J., Nimmannit, U., Callery, P. S., Wang, L. Y., Azad, N., Lipipun, V., Chanvorachote, P. and Rojanasakul, Y. (2006) Reactive oxygen species mediate caspase activation and apoptosis induced by lipoic acid in human lung epithelial cancer cells through Bcl-2 down-regulation. J. Pharmacol. Exp. Ther. 319: 1062-1069. https://doi.org/10.1124/jpet.106.110965