Natural Product Sciences

  • 제17권3호
  • /
  • Pages.221-224
  • /
  • 2011
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  • 1226-3907(pISSN)
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  • 2288-9027(eISSN)
한국생약학회 (The Korean Society of Pharmacognosy)
권호 표지

Neuroprotective Compounds Isolated from the Methanolic Extract of Lonicera japonica

  • Weon, Jin-Bae (Department of Biomaterials Engineering, School of Bioscience and Biotechnology, Kangwon National University) ;
  • Yang, Hye-Jin (Department of Biomaterials Engineering, School of Bioscience and Biotechnology, Kangwon National University) ;
  • Lee, Bo-Hyoung (Department of Biomaterials Engineering, School of Bioscience and Biotechnology, Kangwon National University) ;
  • Yun, Bo-Ra (Department of Biomaterials Engineering, School of Bioscience and Biotechnology, Kangwon National University) ;
  • Choong, Je-Ma (Department of Biomaterials Engineering, School of Bioscience and Biotechnology, Kangwon National University)
  • 투고 : 2011.04.18
  • 심사 : 2011.06.24
  • 발행 : 2011.09.30
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초록

A $CH_3Cl$ extract from the flower of Lonicera japonica (Lauraceae) significantly protected primary cultures of rat cortical cells injured by the excitotoxic amino acid, L-glutamate. Loganin (1), secoxyloganin (2), caffeic acid (3) rutin (4), hyperoside (5), quercetin-3-O-glucoside (6), lonicerin (7), kaempferol-3-O-rutinoside (8), luteolin-7-O-b-D-glucopyranoside (9), quercetin (10) and luteolin (11) were isolated by bioactivity-guided fractionation from the $CH_3Cl$ fraction and further separated using chromatographic techniques. Caffeic acid, lonicerin, kaempferol-3-O-rutinoside, quercetin and luteolin had significant neuroprotective activities against glutamate-induced neurotoxicity in primary cultures of rat cortical cells at concentrations ranging from $0.1{\mu}M$ to $10.0\;{\mu}M$.

키워드

참고문헌

  1. Cacabelos, R., Rodriguez, B., Carrera, C., Caamano, J., Beyer, K., Lao, J.I., and Sellers, M.A., APOE-related frequency of cognitive and noncognitive symptoms in dementia. Meth. Find Exp. Clin. Pharmacol., 18, 693-706 (1996).
  2. Choi, C.W., Jung, H.A., Kang, S.S., and Choi, J.S., Antioxidant constituents and a new triterpenoid glycoside from Flos Lonicerae. Arch. Pharm. Res., 30, 1-7 (2007). https://doi.org/10.1007/BF02977770
  3. Foster, A.C., Gill, R., Kemp, J.A., and Woodruff, G.N., Systemic administration of MK-801 prevents N-methyl-D-aspartate-induced neuronal degeneration in rat brain. Neurosci. Lett., 76, 307-311 (1987). https://doi.org/10.1016/0304-3940(87)90420-4
  4. Heath, P.R. and Shaw, P.J., Update on the glutamatergic neurotransmitter system and the role of excitotoxicity in amyotrophic lateral sclerosis. Muscle Nerve, 26, 438-458 (2002). https://doi.org/10.1002/mus.10186
  5. Jeong, J.J., Ha, Y.M., Jin, Y.C., Lee, E.J., Kim, J.S., Kim, H,J., Seo, H.G., Lee, J.H., K.S., and Chang, K.C., Rutin from Lonicera japonica inhibits myocardial ischemia/reperfusion-induced apoptosis in vivo and protects H9c2 cells against hydrogen peroxide-mediated injury via ERK1/2 and PI3K/Akt signals in vitro. Food Chem. Toxicol., 47, 1569-1576 (2009). https://doi.org/10.1016/j.fct.2009.03.044
  6. Kubo, M. and Ito, E., Structural dynamics of an ionotropic glutamate receptor. Proteins, 56, 411-419 (2004). https://doi.org/10.1002/prot.20154
  7. Kumar, N., Singh, B., Bhandari, P., Gupta, A.P., Uniyal, S.K., and Kaul, V.K., Biflavonoids from Lonicera japonica. Phytochem., 66, 2740-2744 (2005). https://doi.org/10.1016/j.phytochem.2005.10.002
  8. Lee, J.M., Zipfel, G.J., and Choi D.W., The changing landscape of ischaemic brain injury mechanisms. Nature, 399, 7-14 (1999).
  9. Lin, L.M., Zhang, X.G., Zhu, J.J., Gao, H.M., Wang, Z.M., and Wang, W.H., Two new triterpenoid saponins from the flowers and buds of Lonicera japonica. J. Asian. Nat. Prod. Res., 10, 925-929 (2008). https://doi.org/10.1080/10286020802217366
  10. Qi, L-W., Chen, C-Y., and Li, P., Structural characterization and identification of iridoid glycosides, saponins, phenolic acids and flavonoids in Flos Lonicerae Japonicae by a fast liquid chromatography method with diode-array detection and time-of-flight mass spectrometry. Rapid Comm. Mass Spectrom., 23,3227-3242 (2009). https://doi.org/10.1002/rcm.4245
  11. Sheardown, M.J., A new and specific non-NMDA receptor antagonist, FG 9065, blocks L-AP4-evoked depolarization in rat cerebral cortex. Eur. J. Pharmacol., 148, 471-474 (1988). https://doi.org/10.1016/0014-2999(88)90131-8
  12. Tang, D., Li, H.J., Chen, J., Guo, C.W., and Li, P., Rapid and simple method for screening of natural antioxidants from Chinese herb Flos Lonicerae Japonicae by DPPH-HPLC-DAD-TOF/MS. J. Sep. Sci., 31, 3519-3526 (2008). https://doi.org/10.1002/jssc.200800173
  13. Won, J.B. and Ma, C.J., Neuroprotective activities of some medicinal plants against glutamate-induced neurotoxicity in primary cultures of rat cortical cells. Nat. Prod. Sci., 15, 125-129 (2009).
  14. Yu, D.Q., Chen, R.Y., Huang, L.J., Xie, F.Z., Ming, D.S., Zhou, K., Li, H.Y., and Tong, K.M., The structure and absolute configuration of Shuangkangsu: a novel natural cyclic peroxide from Lonicera japonica (Thunb.). J. Asian Nat. Prod. Res., 10, 851-856 (2008). https://doi.org/10.1080/10286020802144677