대한한방부인과학회지 (The Journal of Korean Obstetrics and Gynecology)

  • 제24권3호
  • /
  • Pages.48-72
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  • 2011
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  • 1229-4292(pISSN)
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  • 2508-3619(eISSN)
대한한방부인과학회 (The Society of Korean Medicine Obstetrics and Gynecology)
권호 표지
DOI QR코드

DOI QR Code

용담사간탕(龍膽瀉肝湯)이 Estradiol Valerate로 유발된 흰쥐의 다낭성 난소 발달과 수태에 미치는 영향

Effects of Yongdamsagan-tang on the Progression of the Estradiol Valerate-induced Polycystic Ovaries and on the Conception in Rats

  • 이인재 (세명대학교 한의과대학 한방부인과교실) ;
  • 이동녕 (세명대학교 한의과대학 한방부인과교실) ;
  • 서일복 (세명대학교 한의과대학 한방부인과교실) ;
  • 김형준 (세명대학교 한의과대학 한방부인과교실)
  • Lee, In-Jae (Dept. of Gynecology, Graduate School of Oriental Medicine, Se-Myung Univ.) ;
  • Lee, Dong-Nyung (Dept. of Gynecology, Graduate School of Oriental Medicine, Se-Myung Univ.) ;
  • Seo, Il-Bock (Dept. of Gynecology, Graduate School of Oriental Medicine, Se-Myung Univ.) ;
  • Kim, Hyung-Jun (Dept. of Gynecology, Graduate School of Oriental Medicine, Se-Myung Univ.)
  • 투고 : 2011.07.29
  • 심사 : 2011.08.08
  • 발행 : 2011.08.31
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초록

Objectives: This study was designed to investigate the effects of Yongdamsagantang on the polycystic ovary(PCO) induced by estradiol valerate(EV) in rats. Methods: After administrating Yongdamsagan-tang to PCO induced rats, we measured the weight of body, ovaries, adrenal glands, and uterus of rats. The observation through naked eye and histopathological observation of ovaries were evaluated. Also, the number of follicle and corpora lutea and content of androstenedione(ADD) and total estrogen were evaluated. The expressions of nerve growth factor(NGF) and corticotropin releasing factor(CRF) were analyzed by immunohistochemistry. The breeding rate and number of implantation with normal male rats were evaluated. Results: - The weight(mg) of ovaries in YST treated group($73.8{\pm}7.6$) was significantly increased(p<0.001) compared with control group($54.3{\pm}4.5$). - The number of mature follicles in YST treated group($7.3{\pm}2.4$) was significantly increased(p<0.01) compared with control group($3.5{\pm}1.2$). - The number of atretic follicles in YST treated group($9.0{\pm}1.5$) was significantly decreased(p<0.01) compared with control group($13.4{\pm}3.8$). - The number of cystic follicles in YST treated group($3.1{\pm}1.1$) was significantly decreased(p<0.01) compared with control group($6.0{\pm}2.0$). - The number of corpora lutea in YST treated group($3.8{\pm}2.1$) was significantly increased(p<0.001) compared with control group($0.3{\pm}0.7$). - The expression of NGF-immunoreactive cells in the ovarian granulosa cells in YST treated group was lesser observed than control group. - The expression of NGF-immunoreactive cells in the adrenal cortex in YST treated group was lesser observed than control group. - The breeding rate in YST treated group(100 %) was significantly increased (p<0.05) compared with control group(50 %). - The number of implantation in YST treated group($6.4{\pm}4.7$) was significantly increased(p<0.05) compared with control group($1.4{\pm}2.6$). Conclusions: We concluded that Yongdamsagan-tang activates the maturation of follicles, normal ovulation, breeding rate and number of implantation. Therefore, this may be effective for the treatment of anovulation, amenorrhea and sterility of PCOS patients.

키워드

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