동의생리병리학회지 (Journal of Physiology & Pathology in Korean Medicine)

  • 제25권4호
  • /
  • Pages.608-613
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  • 2011
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  • 1738-7698(pISSN)
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  • 2288-2529(eISSN)
대한동의생리학회·한의병리학회 ( The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine)
권호 표지

녹차추출물이 인체 피지선세포주에서 지질 생성에 미치는 효과

Effect of Green Tea Extract on Lipid Synthesis in Human Sebocyte Cell Line

  • 박시준 (코리아나화장품 송파기술연구소) ;
  • 전병국 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 김대성 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 이강태 (코리아나화장품 송파기술연구소) ;
  • 문연자 (원광대학교 한의과대학) ;
  • 이건국 (코리아나화장품 송파기술연구소) ;
  • 우원홍 (원광대학교 한의학전문대학원 한약자원개발학과)
  • Park, Si-Jun (Coreana Cosmetics Ltd, SongPa R&D Center) ;
  • Jeon, Byoung-Kook (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Kim, Dae-Sung (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Lee, Ghang-Tai (Coreana Cosmetics Ltd, SongPa R&D Center) ;
  • Mun, Yeun-Ja (College of Oriental Medicine, Wonkwang University) ;
  • Lee, Kun-Kuk (Coreana Cosmetics Ltd, SongPa R&D Center) ;
  • Woo, Won-Hong (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University)
  • 투고 : 2011.07.07
  • 심사 : 2011.07.26
  • 발행 : 2011.08.25
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초록

The aqueous Green tea comes to be used with the Oriental medicine plant, it has the numerous health benefits, including antioxidant, anti-inflammatory, and anti- carcinogenic properties. Epidermal progenitor cells give rise to multiple skin lineages: hair follicle, sebaceous gland and the overlying interfollicular epidermis. Sebocytes are the cells of the sebaceous gland, which synthesize and accumulate lipid dropolets. In order to determine the effect of Green tea on lipid production, several experiments were performed in SZ95 cells (sebocytes). We found that Green tea increased lipid droplets compared with control in a dose-dependent manner. Human sebaceous glands produce sebum, a lipid mixture of squalene, wax esters, triglycerides, cholesterol esters, and free fatty acids that is secreted onto the skin. Therefore, to investigate the effects of Green tea on intracellular lipid levels, we treated SZ95 cells with Green tea, and then examined cholesterol and triglyceride levels. After treatment of the cells with Green tea, the cholesterol and triglyceride levels of SZ95 cells were increased significantly in a dose-dependent manner.

키워드

참고문헌

  1. Proksch, E., Fölster-Holst, R., Jensen, J.M. Skin barrier function, epidermal proliferation and differentiation in eczema. J Dermatol Sci. 43(3):159-169, 2006. https://doi.org/10.1016/j.jdermsci.2006.06.003
  2. Pelle, E., McCarthy, J., Seltmann, H., Huang, X., Mammone, T., Zouboulis, C.C., Maes, D. Identification of histamine receptors and reduction of squalene levels by an antihistamine in sebocytes. J Invest Dermatol. 128(5):1280-1285, 2008. https://doi.org/10.1038/sj.jid.5701160
  3. Zouboulis, C.C., Boschnakow, A. Chronological ageing and photoageing of the human sebaceous gland. Clin Exp Dermatol. 26(7):600-607, 2001. https://doi.org/10.1046/j.1365-2230.2001.00894.x
  4. Sato, T., Takahashi, A., Kojima, M., Akimoto, N., Yano, M., Ito, A. A citrus polymethoxy flavonoid, nobiletin inhibits sebum production and sebocyte proliferation, and augments sebum excretion in hamsters. J Invest Dermatol. 127(12):2740-2748, 2007. https://doi.org/10.1038/sj.jid.5700927
  5. Robinson, M., Visscher, M., Laruffa, A., Wickett, R. Natural moisturizing factors (NMF) in the stratum corneum (SC). I. Effects of lipid extraction and soaking. J Cosmet Sci. 61(1):13-22, 2010.
  6. Elias, P.M., Hatano, Y., Williams, M.L. Basis for the barrier abnormality in atopic dermatitis: outside-inside-outside pathogenic mechanisms. J Allergy Clin Immunol. 121(6):1337-1343, 2008. https://doi.org/10.1016/j.jaci.2008.01.022
  7. Eisinger, M., Li, W.H., Anthonavage, M., Pappas, A., Zhang, L., Rossetti, D., Huang, Q., Seiberg, M. A melanocortin receptor 1 and 5 antagonist inhibits sebaceous gland differentiation and the production of sebum-specific lipids. J Dermatol Sci. 63(1):23-32, 2011. https://doi.org/10.1016/j.jdermsci.2011.04.001
  8. Hong, I., Lee, M.H., Na, T.Y., Zouboulis, C.C., Lee, M.O. LXRalpha enhances lipid synthesis in SZ95 sebocytes. J Invest Dermatol. 128(5):1266-1272, 2008. https://doi.org/10.1038/sj.jid.5701134
  9. Komine, M. Analysis of the mechanism for the development of allergic skin inflammation and the application for its treatment:keratinocytes in atopic dermatitis - their pathogenic involvement. J Pharmacol Sci. 110(3):260-264, 2009. https://doi.org/10.1254/jphs.09R06FM
  10. Schmidt, A., Kimmel, D.B., Bai, C., Scafonas, A., Rutledge, S., Vogel, R.L., McElwee-Witmer, S., Chen, F., Nantermet, P.V., Kasparcova, V., Leu, C.T., Zhang, H.Z., Duggan, M.E., Gentile, M.A., Hodor, P., Pennypacker, B., Masarachia, P., Opas, E.E., Adamski, S.A., Cusick, T.E., Wang, J., Mitchell, H.J., Kim, Y., Prueksaritanont, T., Perkins, J.J., Meissner, R.S., Hartman, G.D., Freedman, L.P., Harada, S., Ray, W.J. Discovery of the selective androgen receptor modulator MK-0773 using a rational development strategy based on differential transcriptional requirements for androgenic anabolism versus reproductive physiology. J Biol Chem. 285(22):17054-17064, 2010. https://doi.org/10.1074/jbc.M109.099002
  11. Makrantonaki, E., Ganceviciene, R., Zouboulis, C. An update on the role of the sebaceous gland in the pathogenesis of acne. Dermatoendocrinol. 3(1):41-49, 2011. https://doi.org/10.4161/derm.3.1.13900
  12. Ziaedini, A., Jafari, A., Zakeri, A. Extraction of antioxidants and caffeine from green tea (Camelia sinensis) leaves: kinetics and modeling. Food Sci Technol Int. 16(6):505-510, 2010. https://doi.org/10.1177/1082013210367567
  13. Jagdeo, J., Brody, N. Complementary antioxidant function of caffeine and green tea polyphenols in normal human skin fibroblasts. J Drugs Dermatol. 10(7):753-761, 2011.
  14. Saito, S.T., Gosmann, G., Pungartnik, C., Brendel, M. Green tea extract-patents and diversity of uses. Recent Pat Food Nutr Agric. 1(3):203-215, 2009. https://doi.org/10.2174/2212798410901030203
  15. 中藥大辭典, 김창민, 신민교, 안덕균, 이경순 외. 정담. 2: 839-840.
  16. Mosmann, T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 65(1-2):55-63, 1983. https://doi.org/10.1016/0022-1759(83)90303-4
  17. 조규형, Aruna, J., Tomohiko, T., 김종철, 김루미, 윤호성, 김경태. STS-RFLP법을 이용한 국내지역 재배녹차의 비교분석. 생명과학회지 20(9):1287-1425, 2010.
  18. Khan, N., Mukhtar, H. Cancer and metastasis: prevention and treatment by green tea. Cancer Metastasis Rev. 29(3):435-445, 2010. https://doi.org/10.1007/s10555-010-9236-1
  19. Heinrich, U., Moore, C.E., De Spirt, S., Tronnier, H., Stahl, W. Green tea polyphenols provide photoprotection, increase microcirculation, and modulate skin properties of women. J Nutr. 141(6):1202-1208, 2011. https://doi.org/10.3945/jn.110.136465
  20. Castro, J., Pregibon, T., Chumanov, K., Marcus, R.K. Determination of catechins and caffeine in proposed green tea standard reference materials by liquid chromatography-particle beam/electron ionization mass spectrometry (LC-PB/EIMS). Talanta. 82(5):1687-1695, 2010. https://doi.org/10.1016/j.talanta.2010.07.054
  21. Zouboulis, C.C., Baron, J.M., Böhm, M., Kippenberger, S., Kurzen, H., Reichrath, J., Thielitz, A. Frontiers in sebaceous gland biology and pathology. Exp Dermatol. 17(6):542-551, 2008. https://doi.org/10.1111/j.1600-0625.2008.00725.x
  22. El-Mowafy, A.M., Salem, H.A., Al-Gayyar, M.M., El-Mesery, M.E., El-Azab, M.F. Evaluation of renal protective effects of the green-tea (EGCG) and red grape resveratrol: role of oxidative stress and inflammatory cytokines. Nat Prod Res. 25(8):850-856, 2011. https://doi.org/10.1080/14786419.2010.533669
  23. Tansirikongkol, A., Wickett, R.R., Visscher, M.O., Hoath, S.B. Effect of vernix caseosa on the penetration of chymotryptic enzyme: potential role in epidermal barrier development. Pediatr Res. 62(1):49-53, 2007. https://doi.org/10.1203/PDR.0b013e318067b442
  24. Oudshoorn, M.H., Rissmann, R., van der Coelen, D., Hennink, W.E., Ponec, M., Bouwstra, J.A. Effect of synthetic vernix biofilms on barrier recovery of damaged mouse skin. Exp Dermatol. 18(8):695-703, 2009. https://doi.org/10.1111/j.1600-0625.2009.00858.x
  25. Sansone, G., Reisner, R.M. Differential rates of conversion of testosterone to dihydrotestosterone in acne and in normal human skin--a possible pathogenic factor in acne. J Invest Dermatol. 56(5):366-372, 1971. https://doi.org/10.1111/1523-1747.ep12261252
  26. Downing, D.T., Strauss, J.S., Pochi, P.E. Variability in the chemical composition of human skin surface lipids. J Invest Dermatol. 53(5):322-327, 1969. https://doi.org/10.1038/jid.1969.157
  27. Webster, G.F. Inflammation in acne vulgaris. J Am Acad Dermatol. 33: 247-253, 1995. https://doi.org/10.1016/0190-9622(95)90243-0
  28. Ramasastry, P., Downing, D.T., Pochi, P.E., Strauss, J.S. Chemical composition of human skin surface lipids from birth to puberty. J Invest Dermatol. 54(2):139-144, 1970. https://doi.org/10.1111/1523-1747.ep12257164
  29. Pappas, A., Anthonavage, M., Gordon, J.S. Metabolic fate and selective utilization of major fatty acids in human sebaceous gland. J Invest Dermatol. 118(1):164-171, 2002. https://doi.org/10.1046/j.0022-202x.2001.01612.x
  30. Ge, L., Gordon, J.S., Hsuan, C., Stenn, K., Prouty, S.M. Identification of the delta-6 desaturase of human sebaceous glands: expression and enzyme activity. J Invest Dermatol. 120(5):707-714, 2003. https://doi.org/10.1046/j.1523-1747.2003.12123.x
  31. Thiboutot, D. Regulation of human sebaceous glands. J Invest Dermatol. 123(1):1-12, 2004. https://doi.org/10.1111/j.1523-1747.2004.t01-2-.x
  32. Darley, C.R., Moore, J.W., Besser, G.M., Munro, D.D., Edwards, C.R., Rees, L.H., Kirby, J.D. Androgen status in women with late onset or persistent acne vulgaris. Clin Exp Dermatol. 9(1):28-35, 1984. https://doi.org/10.1111/j.1365-2230.1984.tb00751.x
  33. De Raeve, L., De Schepper, J., Smitz, J. Prepubertal acne: a cutaneous marker of androgen excess?. J Am Acad Dermatol. 32(2 Pt 1):181-184, 1995. https://doi.org/10.1016/0190-9622(95)90122-1
  34. Sakai, S., Kikuchi, K., Satoh, J., Tagami, H., Inoue, S. Functional properties of the stratum corneum in patients with diabetes mellitus: similarities to senile xerosis. Br J Dermatol. 153(2):319-323, 2005. https://doi.org/10.1111/j.1365-2133.2005.06756.x