Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Dietary Vitamin E Influences the Levels of Nitric Oxide and Cytokines in Broiler Chickens

  • Xu, Jian-Xiong (School of Agriculture and Biology, Shanghai Jiao Tong University) ;
  • Chen, Xiao-Lian (School of Agriculture and Biology, Shanghai Jiao Tong University) ;
  • Wang, Jing (School of Agriculture and Biology, Shanghai Jiao Tong University) ;
  • Wang, Tian (College of Animal Science and Technology, Nanjing Agricultural University)
  • Received : 2011.02.20
  • Accepted : 2011.04.27
  • Published : 2011.10.01
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Abstract

The study investigated the effects of dietary Vitamin E (VE) on nitric oxide (NO) metabolism, immune function and analyzed the correlation between NO free radical and cytokines (IL-2 and IL-6) in broilers. One hundred and fifty 2-week-old broilers were randomly divided into three groups. Control group and lower VE ($VE^-$) group were provided with a basic diet supplemented with 12.55 mg/kg VE and 2.55 mg/kg VE for 30 days, respectively. Higher VE ($VE^{-}-VE^+$) group was supplemented with 2.55 mg/kg VE in the first 15 days and then 32.55 mg/kg VE in the next 15 days. Five broilers in each group were then sacrificed on the 5th, 10th, 15th, 20th, 25th and 30th days, respectively, and the content of NO free radical, superoxide dismutase (SOD), glutathione peroxidase (GSHPx), malondialdehyde (MDA) and cytokines, IL-2 and IL-6, were measured. The results showed that lower VE could decrease growth performance of broilers while higher VE could increase growth performance and eliminate differences resulted from feeding lower VE dietary in early stages (p<0.05). Compared with the control group, lower VE could increase significantly NO and MDA concentration, and increase IL-2 concentration in serum (p<0.05). Higher VE could significantly increase activities of SOD and glutathione GSH-Px (p<0.05). IL-2 is positively correlated with NO in heart (p<0.05) and IL-6 is negatively correlated with NO in liver (p<0.05) and heart (p<0.01). These results indicate that dietary VE could regulate antioxidant capacity and NO metabolism of broilers and higher VE-supplemented diet could directly decrease production of IL-2.

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References

  1. Brigelius-Flohe, R., F. J. Kelly, J. T. Salonen, J. Neuzil, J. M. Zingg and A. Azzi. 2002. The European perspective on vitamin E: current knowledge and future research. Am. J. Clin. Nutr. 76:703-716.
  2. Fang, Y. Z. and W. J. Li. 1989. Free radicals and enzymes - the theoretical basis and its application in biology and medicine. Science Press, Beijing, China.
  3. Fang, Y. Z. and R. L. Zheng. 2002. The theories and applications of free radical biology. Science press, Beijing, China.
  4. Geng, Y. J., K. Hellstrand, A. Wennmalm and G. K. Hansson. 1996. Apoptotic death of human leukemic cells induced by vascular cells expressing nitric oxide synthase in response to gamma-interferon and tumor necrosis factor-alpha. Cancer Res. 56:866-874.
  5. Guo, Q., G. Rimbach, H. Moinic, S. Weber and L. Packer. 2002. ESR and cell culture studies on free radical-scavenging and antioxidant activities of isoflavonoids. Toxicology 179:171-180. https://doi.org/10.1016/S0300-483X(02)00241-X
  6. Hafeman, D. G., R. A.Sunde and W. G. Hoekstra. 1974. Effect of dietary selenium on erythrocyte and liver glutathione peroxidase in the rats. J. Nutr. 104:580-587.
  7. Han, B. and X. L. Wang. 2002. Effects of low temperature on free radical metabolism in broilers with ascites syndrome. Chin. J. Anim. Sci. Vet. Med. 33:327-331.
  8. Huang, N. and B. Y. Wang. 2000. Advance in gene transcriptional factor $NF-{\kappa}B$ and its significance in medicine. Sichuan J. Physiol. Sci. 22:2-5.
  9. Jin, J. and G. T. Liu. 2000. Research progress in $NF-{\kappa}B$ studies. Foreign Med. Sci. (Pharm). 27:133-137.
  10. Koshland, D. E. J. 1992. The molecular of the year. Science 258:1861. https://doi.org/10.1126/science.1470903
  11. Lander, H. M., J. S. Ogiste, S. F. A. Pearce, R. Levi and A. Novogrodsky. 1995. Nitric Oxide-stimulated Guanine Nucleotide Exchange on $p21^{ras}$. J. Biol. Chem. 270:7017-7020. https://doi.org/10.1074/jbc.270.13.7017
  12. Leshchinsky, T. V. and K. C. Klasing. 2001. Relationship between the level of dietary vitamin E and the immune response of broiler chickens. Poult. Sci. 80:1590-1599. https://doi.org/10.1093/ps/80.11.1590
  13. Liu, J. C. and W. Y. Qu. 2001. Research progress in vitamin E. Chin. J. Hosp. Pharm. 21:116-117.
  14. Morrissey, P. A., S. Brandon, D. J. Buckley, P. J. A. Sheehy and M. Frigg. 1997. Tissue content of $\alpha$-tocopherol and oxidative stability of broilers receiving dietary $\alpha$-tocopheryl acetate supplement for various periods pre-slaughter. Br. Poult. Sci. 38:84-88. https://doi.org/10.1080/00071669708417945
  15. Peng, H. B., P. Libby and J. K. Liao. 1995. Induction and stabilization of I kappa B alpha by nitric oxide mediated inhibition of NF-kappa B. J. Biol. Chem. 270:14214-14219. https://doi.org/10.1074/jbc.270.23.14214
  16. Reiss, U. and D. Gershon. 1976. Comparison of cytoplasmic superoxide dismutase in liver, heart and brain of aging rats and mice. Biochem. Biophys. Res. Commun. 73:255-262. https://doi.org/10.1016/0006-291X(76)90701-4
  17. Placer, Z. A., L. L. Cushman and B. C. Johnson. 1996. Estimation of lipid peroxidation, malindialdehyde in biochemical system. Anal. Biochem. 16:359-367.
  18. Rossi, F. 1986. The $O_2^-$-forming NADPH oxidase of the phagocytes: nature, mechanisms of activation and function. Biochim. Biophys. Acta 853:65-89. https://doi.org/10.1016/0304-4173(86)90005-4
  19. Roy, R. S. and J. M. McCord. 1982. Ischemia-induced conversion of xanthine dehydrogenase to xanthine oxidase. Fed. Proc. 41:767-772.
  20. Stasi, R., P. L. Zinzani, P. Galieni, V. M. Lauta, E. Damasio, E. Dispensa, F. Dammacco, A. Venditti, G. Del Poeta, M. Cantonetti, A. Perrotti, G. Papa and S. Tura. 1995. Clinical implications of cytokine and soluble receptor measurements in patients with newly-diagnosed aggressive non-Hodgkin's lymphoma. Eur. J. Haematol. 54:9-17.
  21. Tian, Y., X. Y. Lu and X. J. He. 2005. Properties of natural plant antioxidant in scavenging oxygen free radicals. Food Sci. 26:123-126.
  22. Van Snick, J. 1990. Interleukin-6: an overview. Annu. Rev. Immunol. 8:253-278. https://doi.org/10.1146/annurev.iy.08.040190.001345
  23. Vercammen, C. and J. L. Ceuppens. 1987. Prostaglandin E2 inhibits human T-cell proliferation after crosslinking of the CD3-Ti complex by directly affecting T cells at an early step of the activation process. Cell Immunol. 104:24-36. https://doi.org/10.1016/0008-8749(87)90003-7
  24. Winterbourn, C. C., R. E. Hawkins, M. Brain and R. Carrell. 1975. The estimation of red cell superoxide dismutase activity. J. Lab. Clin. Med. 85:337-341.
  25. Xu, J. X., J. Wang and T. Wang. 2007. Effects of vitamin E and selenium on the metabolism of different free radicals in broilers. Chin. J. Appl. Ecol. 18:1789-1993.
  26. Zhang, S. J., X. Y. Wang, J. X. Li, Z. M. Qi, S. P. Li, J. R. Meng, Y. W. Wang and Y. Zhu. 1998. The roles of vitamin E in free radical metabolism in selenium-deficient animals. Chin. J. Vet. 18:15-17.
  27. Zhu, H., H. L. Luo, H. Meng, G. J. Zhang, L. Y. Yan and D. B. Yue. 2010. Effect of vitamin E supplement in diet on antioxidant ability of testis in Boer goat. Anim. Reprod. Sci. 117:90-94. https://doi.org/10.1016/j.anireprosci.2009.03.016

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